Effect of equol intervention on high-fat diet-induced non-alcoholic fatty liver disease in ovariectomized rats

Guiming ZHANG; Xiangmin NI; Hanqiang CUI; Zhe XU; Shuo LI; Jian WANG

doi:10.16016/j.2097-0927.202204122

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Effect of equol intervention on high-fat diet-induced non-alcoholic fatty liver disease in ovariectomized rats

Basic Medicine | 更新时间：2026-01-09

- Effect of equol intervention on high-fat diet-induced non-alcoholic fatty liver disease in ovariectomized rats
- Journal of Army Medical University Vol. 44, Issue 21, Pages: 2129-2137(2022)
- 作者机构：
  
  400037 重庆，陆军军医大学(第三军医大学)第二附属医院营养科
- 作者简介：
- 基金信息：
  
  the General Program of National Nature Science Foundation of China(81973040)
- DOI：10.16016/j.2097-0927.202204122
  CLC： R285.5;R459.3;R575.5
- Received：16 April 2022，
  
  Revised：2022-06-17，
  
  Published：15 November 2022
- 稿件说明：
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Guiming ZHANG, Xiangmin NI, Hanqiang CUI, et al. Effect of equol intervention on high-fat diet-induced non-alcoholic fatty liver disease in ovariectomized rats[J]. Journal of Army Medical University, 2022, 44(21): 2129-2137.
DOI：

Guiming ZHANG, Xiangmin NI, Hanqiang CUI, et al. Effect of equol intervention on high-fat diet-induced non-alcoholic fatty liver disease in ovariectomized rats[J]. Journal of Army Medical University, 2022, 44(21): 2129-2137. DOI： 10.16016/j.2097-0927.202204122.

摘要

目的

观察雌马酚(equol

Eq)干预对高脂饮食诱导的去卵巢大鼠非酒精性脂肪肝的影响，并探讨其作用机制。

方法

5周龄雌性SD大鼠60只，采用随机数字表法分为6组：假手术组

卵巢切除(ovariectomized

OVX)+高脂饲料(high-fat diet，HFD)喂养组，OVX+HFD+低、中、高剂量[20、40、80 mg/(kg·d)]Eq干预组，OVX+HFD+雌二醇[estradiol，E2，0.25 mg/(kg·d)]干预组。干预12周后

处死大鼠并取材。计算肝指数

检测大鼠血清脂质(TG、TC、HDL-c、LDL-c、FFA)、肝功能(ALT、AST)、炎症因子(TNF-α、IL-6)、雌激素、空腹胰岛素(fasting insulin

FINS)、空腹血糖(fasting plasma glucose

FPG)及肝脏TG、TC、TNF-α、IL-6含量，HE染色观察肝脏组织形态

qRT-PCR检测肝脏TNF-α、IL-6 mRNA表达，Western blot检测肝脏NF-κB p-p65蛋白表达。

结果

与假手术组比较，OVX+HFD组大鼠血清雌激素水平显著下降(

0.05)，出现明显的肝脂肪变性、脂质代谢紊乱、肝功能异常和炎症水平升高；与OVX+HFD组比较，Eq或E2干预后，大鼠血清雌激素、HDL-c水平显著升高，血清TG、TC、HDL-c、LDL-c、FFA、ALT、AST、TNF-α、IL-6水平显著降低(

0.05)，肝组织NF-κB p-p65、TNF-α、IL-6表达水平显著降低(

0.05)，不同程度地改善了肝组织脂肪变性。

结论

Eq干预能有效改善高脂饮食诱导的去卵巢大鼠非酒精性脂肪肝

可能与Eq抑制炎症因子表达有关。

Abstract

Objective

To determine the effect of equol (Eq) intervention on high-fat diet-induced non-alcoholic fatty liver disease (NAFLD) in ovariectomized rats and investigate its mechanism of action.

Methods

Sixty 5-week-old female SD rats were randomly divided into 6 groups: sham group

ovariectomized+high-fat diet (OVX +HFD) group

OVX+HFD+low-

medium-

and high-dose Eq groups [20

40 and 80 mg/(kg·d) Eq]

and OVX+ HFD+estradiol group [E2

0.25 mg/(kg·d)]

with 8 rats in each group. After 12 weeks of intervention

the rats were sacrificed and sampled. The liver index was calculated and the serum levels of blood lipid TG

HDL-C

LDL-C

FFA

liver function in indicators ALT

AST

inflammatory factors TNF-α

IL-6

estrogen

fasting insulin (FINS)

fasting plasma glucose (FPG) and liver contents of TG

TNF-α and IL-6 were detected. HE staining was used to observe the morphology of liver tissue. qRT-PCR and Western blotting was employed to detect the mRNA expression of TNF-α and IL-6 and the protein levels of NF-κB p-p65 in the liver tissues

respectively.

Results

Compared with the sham group

the OVX+HFD group had significantly decreased serum estrogen level (

0.05) and obvious hepatic steatosis

dyslipidemia

abnormal liver function and increased levels of inflammatory factors. Eq or E2 intervention resulted in notably increased serum estrogen and HDL-C levels

and remarkably reduced serum TG

HDL-C

LDL-C

FFA

ALT

AST

TNF-α and IL-6 levels (

0.05)

down-regulated expression of NF-κB p-p65

TNF-α and IL-6 in the liver tissues (

0.05)

and certain improvements in steatosis of liver tissues when compared with the OVX+HFD group.

Conclusion

Eq intervention can effectively ameliorate high-fat diet-induced NAFLD in ovariectomized rats

and the mechanism may be partially associated with its inhibition on expression of inflammatory factors.

关键词

Keywords

references

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