STX17 improves Aβ31-35 induced death of hippocampal neurons in mice

Ning WANG; Xiuya ZHOU; Xinghong HE; Lifei ZHANG; Pengjia LI; Shu SHI; Li WANG; Xiaohui WANG

doi:10.16016/j.2097-0927.202203123

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STX17 improves Aβ31-35 induced death of hippocampal neurons in mice

Basic Medicine | 更新时间：2026-01-09

- STX17 improves Aβ31-35 induced death of hippocampal neurons in mice
- Journal of Army Medical University Vol. 44, Issue 17, Pages: 1712-1719(2022)
- 作者机构：
  
  1.030001 太原，山西医科大学基础医学院：病理教研室
  2.030001 太原，山西医科大学基础医学院：基础医学研究中心
- 作者简介：
- 基金信息：
  
  the General Program of Natural Foundation for Applied Basic Research Plan of Shanxi Province(201901D111197);the Excellent Achievements (Science and Technology) Cultivation Project of Colleges and Universities in Shanxi Province(2020KJ012);the "1331 Project" for Key-Discipline Construction Plan for Advantageous and Characteristic Disciplines of Shanxi Province(1331KSC);the Scientific Research Project for Returning Overseas Students of Shanxi Province(2020-082)
- DOI：10.16016/j.2097-0927.202203123
  CLC： R338.26;R341;R394.2
- Received：14 March 2022，
  
  Revised：2022-05-06，
  
  Published：15 September 2022
- 稿件说明：
移动端阅览
Ning WANG, Xiuya ZHOU, Xinghong HE, et al. STX17 improves Aβ31-35 induced death of hippocampal neurons in mice[J]. Journal of Army Medical University, 2022, 44(17): 1712-1719.
DOI：

Ning WANG, Xiuya ZHOU, Xinghong HE, et al. STX17 improves Aβ31-35 induced death of hippocampal neurons in mice[J]. Journal of Army Medical University, 2022, 44(17): 1712-1719. DOI： 10.16016/j.2097-0927.202203123.

摘要

目的

探究突触融合蛋白17(syntaxin17，STX17)在Aβ诱导的小鼠海马神经细胞死亡中的作用。

方法

基于WGCNA方法选取与阿尔茨海默病显著相关的基因，与自噬基因库和SNARE家族基因取交集筛选关键基因；C57BL/6小鼠海马组织中注射浓度为1 g/L的Aβ31-35和使用终浓度为5 μmol/L Aβ31-35处理HT22海马神经细胞，采用Western Blot检测STX17、LC3Ⅱ、P62的蛋白表达情况；CCK-8法检测Aβ31-35处理的HT22海马神经细胞存活率；慢病毒介导HT22海马神经细胞STX17过表达后，Western blot检测Aβ31-35处理后LC3Ⅱ、P62的蛋白表达情况。

结果

与阿尔茨海默病显著相关的Salmon和Black模块分别与自噬基因库和SNARE亚家族取交集获取关键基因STX17。与对照组比较，经Aβ31-35处理后，HT22细胞细胞存活率[(81.81±11.65)%

]

明显下降(

0.05)，小鼠海马组织和HT22细胞中STX17蛋白表达均显著降低(

0.05)，LC3Ⅱ和P62蛋白表达均显著升高(

0.05)；慢病毒介导HT22海马神经细胞STX17过表达后，显著逆转Aβ31-35诱导的LC3Ⅱ、P62的蛋白表达上调(

0.05)，并改善Aβ31-35诱导的HT22海马神经细胞存活率下降[(101.91±13.81)%

(79.21±8.75)%，

0.05

]

。

结论

STX17通过促进自噬来改善Aβ诱导的小鼠海马神经细胞死亡。

Abstract

Objective

To explore the role of STX17 protein in Aβ-induced death of mouse hippocampal neuronal cells.

Methods

Based on Weighted correlation network analysis (WGCNA)

the modules significantly related to Alzheimer's disease (AD) were selected

and key genes were screened for cross-fertilization with autophagy gene bank and SNARE family genes. Aβ31-35 at a concentration of 1 g/L was used to inject the C57BL/6 mouse hippocampal tissue

and HT22 hippocampal neuronal cells were treated with 5 μmol/L Aβ31-35. Then the protein expression of STX17

LC3 Ⅱ and P62 was detected by Western blotting. The survival rate of the HT22 hippocampal neuronal cells treated by using Aβ31-35 was detected by CCK-8 assay. Protein expression of LC3Ⅱ and P62 were determined by Western blotting after overexpression STX17 in HT22 hippocampal neuronal cells.

Results

The Salmon and Black modules significantly associated with AD crossed with autophagic gene pool and SNARE subfamily intersection STX17

respectively. Under the action of Aβ31-35 mouse hippocampal tissue and mouse HT22 hippocampal neuronal cells

and the survival rate of HT22 cells [(81.81±11.65)%

(100.00±12.89)%

0.05

]

were decreased significantly

and the expression of STX17 was significantly reduced in both tissue and cells (

0.05). The expression of tissue LC3Ⅱ and P62 proteins were significantly elevated in the tissue (

0.05)

and in the cells (

0.05). After lentivirus-mediated overexpression of STX17 in HT22 hippocampal neuronal cells

the protein expression upregulation of LC3II and P62 induced by Aβ31-35 was significantly reversed

and the survival rate was improved in Aβ31-35-induced HT22 hippocampal neuronal cells [(101.91±13.81)%

(79.21±8.75)%

0.05

]

Conclusion

STX17 improves Aβ-induced death of mouse hippocampal neuronal cells by promoting autophagy.

关键词

Keywords

references

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