Heterogeneity of epithelial-mesenchymal transition and CD26 expression in hypertrophic scars and keloids

Wanru GONG; Mengchang LIU; Xingke LIU; Defu XIE; Hong YAN

doi:10.16016/j.2097-0927.202203103

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Heterogeneity of epithelial-mesenchymal transition and CD26 expression in hypertrophic scars and keloids

Clinical Medicine | 更新时间：2026-01-09

- Heterogeneity of epithelial-mesenchymal transition and CD26 expression in hypertrophic scars and keloids
- Journal of Army Medical University Vol. 44, Issue 21, Pages: 2174-2182(2022)
- 作者机构：
  
  646000 四川泸州，西南医科大学附属医院整形烧伤外科, 国家临床重点建设专科, 创面修复与再生实验室
- 作者简介：
- 基金信息：
  
  the Joint Project of Strategic Cooperation in Science and Technology of Luzhou People's Government-Southwest Medical University(2019LZXNYDZ08)
- DOI：10.16016/j.2097-0927.202203103
  CLC： R322.99;363.21;R619.6
- Received：11 March 2022，
  
  Revised：2022-04-10，
  
  Published：15 November 2022
- 稿件说明：
移动端阅览
Wanru GONG, Mengchang LIU, Xingke LIU, et al. Heterogeneity of epithelial-mesenchymal transition and CD26 expression in hypertrophic scars and keloids[J]. Journal of Army Medical University, 2022, 44(21): 2174-2182.
DOI：

Wanru GONG, Mengchang LIU, Xingke LIU, et al. Heterogeneity of epithelial-mesenchymal transition and CD26 expression in hypertrophic scars and keloids[J]. Journal of Army Medical University, 2022, 44(21): 2174-2182. DOI： 10.16016/j.2097-0927.202203103.

摘要

目的

通过对比分析增生性瘢痕、浅表扩张型瘢痕疙瘩的边缘和中心及正常皮肤E-cadherin、Vimentin、基质金属蛋白酶-9(matrix metalloprotein-9，MMP-9)、肿瘤坏死因子-α(tumor necrosis factor alpha

TNF-α)、二肽基肽酶-4 (dipeptidyl-peptidase 4，DPP4，又称CD26)的表达以及组织学上的差异。

方法

纳入2020年10月至2021年8月本院整形烧伤外科行瘢痕疙瘩及增生性瘢痕切除手术患者各10例，收集其切除的瘢痕疙瘩及增生性瘢痕组织，另取需要进行部分正常皮肤切除手术的8例患者正常皮肤组织。HE染色观察各组织形态，免疫组化和Western blot检测E-cadherin、Vimentin、MMP-9、TNF-α、CD26在组织中的表达。

结果

免疫组化及Western blot检测结果显示瘢痕疙瘩和增生性瘢痕表皮层的E-cadherin表达均较正常皮肤明显减弱(

0.05)，瘢痕疙瘩边缘较中心明显减弱(

0.05)，增生性瘢痕边缘与中心无明显差异；瘢痕疙瘩和增生性瘢痕表皮层中的Vimentin、MMP-9、TNF-α、CD26表达较正常皮肤明显增强(

0.05)，瘢痕疙瘩边缘较中心明显增强(

0.05)，增生性瘢痕边缘与中心无明显差异。

结论

上皮间质转化(epithelium-to-mesenchymal transition，EMT)程度及在个体瘢痕内部的差异可能是增生性瘢痕与瘢痕疙瘩临床表现差异的原因之一，TNF-α及CD26可能是其中的关键因子。

Abstract

Objective

To investigate the histological differences and expression of E-cadherin

Vimentin

matrix metalloprotein-9 (MMP-9)

TNF-α

dipeptidyl-peptidase 4 (DPP4

also called CD26) in the margin and center of hypertrophic scar

superficial dilated keloid and normal skin.

Methods

Ten patients with keloid and another 10 with hypertrophic scar undergoing excision surgery in our hospital from October 2020 to August 2021 were included

and 8 cases requiring partial normal skin resection were also recruited. The resected tissues were collected

the tissue morphology was observed with HE staining

and the expression levels of E-cadherin

Vimentin

MMP-9

TNF-α and CD26 were detected by immunohistochemical staining and Western blotting.

Results

The E-cadherin expression in the epidermis of both keloid and hypertrophic scar was significantly lower than that of normal skin (

0.05)

and the level in the edge of keloid was obviously weakened as compared with that of the center (

0.05)

but there was no significant difference between the edge of hypertrophic scar and the center (

0.05). By contrast

the expression levels of Vimentin

MMP-9

TNF-α and CD26 in the epidermis of both keloid and hypertrophic scar were remarkably enhanced when compared with those of normal skin (

0.05)

and the keloids' edge higher than the center (

0.05)

while no significance between the hypertrophic scars' edge and center (

0.05).

Conclusion

The differences in epithelial-to-mesenchymal transition (EMT) and expression of relevant biomarkers within individual scars may be one of the reasons for the diversity of clinical features between hypertrophic scar and keloid

in which TNF-α and CD26 may be the key factors.

关键词

Keywords

references

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