Effect of RNA sensor IFIH1 on proliferation, migration and invasion of HPV positive cervical cancer cells

Lin YU; Ying LUO; Di WU; Shiyuan HONG

doi:10.16016/j.2097-0927.202203089

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Effect of RNA sensor IFIH1 on proliferation, migration and invasion of HPV positive cervical cancer cells

Basic Medicine | 更新时间：2026-01-09

- Effect of RNA sensor IFIH1 on proliferation, migration and invasion of HPV positive cervical cancer cells
- Journal of Army Medical University Vol. 44, Issue 16, Pages: 1651-1660(2022)
- 作者机构：
  
  400016 重庆，重庆医科大学生命科学研究院
- 作者简介：
- 基金信息：
- DOI：10.16016/j.2097-0927.202203089
  CLC： R341;R730.23;R737.33
- Received：10 March 2022，
  
  Revised：2022-05-03，
  
  Published：30 August 2022
- 稿件说明：
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Lin YU, Ying LUO, Di WU, et al. Effect of RNA sensor IFIH1 on proliferation, migration and invasion of HPV positive cervical cancer cells[J]. Journal of Army Medical University, 2022, 44(16): 1651-1660.
DOI：

Lin YU, Ying LUO, Di WU, et al. Effect of RNA sensor IFIH1 on proliferation, migration and invasion of HPV positive cervical cancer cells[J]. Journal of Army Medical University, 2022, 44(16): 1651-1660. DOI： 10.16016/j.2097-0927.202203089.

摘要

目的

探讨RNA传感器干扰素诱导的解旋酶C结构域蛋白1(interferon-induced helicase C domain protein 1，IFIH1)对人乳头瘤病毒18型(human papillomavirus 18，HPV18)阳性的人宫颈癌细胞HeLa增殖、迁移和侵袭的影响。

方法

利用GEPIA数据库分析IFIH1在正常宫颈组织和宫颈癌组织中的表达。Western blot检测IFIH1在C33A和HeLa细胞中的表达。将HPV18基因组转染到正常上皮细胞HaCaT中，并利用Western blot和实时定量PCR检测IFIH1的表达水平。利用实时无标记动态细胞分析技术和CCK8法检测细胞的增殖能力。划痕愈合实验、Transwell迁移实验和侵袭实验分别检测细胞的迁移能力和侵袭能力。Western blot和免疫荧光实验检测STAT3和磷酸化STAT3的蛋白表达和定位。

结果

GEPIA数据库分析显示IFIH1在宫颈癌组织中的表达量明显高于正常宫颈组织(

0.05)；相比于C33A细胞，HeLa细胞中IFIH1的表达水平明显增加(

0.01)；与正常HaCaT细胞相比，转染HPV18的HaCaT细胞中IFIH1的表达增加(

0.01)；与阴性对照相比，敲低IFIH1明显抑制HeLa细胞的增殖、迁移和侵袭能力，而过表达IFIH1则促进C33A细胞的增殖、迁移和侵袭(

0.01)；敲低IFIH1后，磷酸化STAT3蛋白的表达减少(

0.01)。

结论

IFIHI在被HPV感染的宫颈癌细胞中表达增加，这将促进细胞的增殖、迁移和侵袭能力，可能与激活STAT3有关。

Abstract

Objective

To investigate the effect of RNA sensor interferon-induced helicase C domain protein 1 (IFIH1) on the proliferation

migration and invasion of HPV18 positive human cervical cancer HeLa cell line.

Methods

The Gene Expression Profiling Interactive Analysis (GEPIA) database was used to analyze the expression of IFIH1 in normal cervical tissues and cervical cancer tissues. The expression of IFIH1 in C33A cells and HeLa cells was detected by Western blotting. After the HPV18 genome was transfected into normal epithelial HaCaT cells

the expression of IFIH1 was detected by Western blotting and real-time quantitative PCR. The ability of cell proliferation was detected by real time cell analysis (RTCA) system and CCK8 assay

and the ability of cell migration and invasion were examined by wound scratch healing assay and transwell assay. The expression and localization of STAT3 and phosphorylated STAT3 were analyzed by Western blotting and immunofluorescence assay.

Results

GEPIA database analysis showed that the expression of IFIH1 was significantly higher in cervical cancer tissues than normal cervical tissues (

0.05). Compared with the C33A cells

the expression of IFIH1 in HeLa cells was significantly increased (

0.01). And its expression was increased in the HaCaT cells with HPV18 genome transfection than the normal HaCaT cells (

0.01). IFIH1 knockdown resulted in obviously decreased proliferation

migration and invasion abilities in HeLa cells when compared with the negative control cells

while overexpression of IFIH1 promoted the proliferation

migration and invasion in the C33A cells (

0.01). Meanwhile

the expression of phosphorylated STAT3 protein was decreased in HeLa cells with IFIH1 knockdown (

0.01).

Conclusion

The expression of IFIH1 is increased in cervical cancer cells infected by HPV

which promotes cell proliferation

migration and invasion and may be related to the activation of STAT3.

关键词

Keywords

references

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