Exosomes derived from bone marrow mesenchymal stem cells ameliorate peritoneal fibrosis induced by high glucose peritoneal dialysate solution in mice

Xiaoyue WANG; Qingli CAI; Fang YU; Jia LUO; Liming WANG; Jia CHEN; Kehong CHEN; Yani HE

doi:10.16016/j.2097-0927.202202132

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Exosomes derived from bone marrow mesenchymal stem cells ameliorate peritoneal fibrosis induced by high glucose peritoneal dialysate solution in mice

Basic Medicine | 更新时间：2026-01-09

- Exosomes derived from bone marrow mesenchymal stem cells ameliorate peritoneal fibrosis induced by high glucose peritoneal dialysate solution in mice
- Journal of Army Medical University Vol. 44, Issue 22, Pages: 2291-2299(2022)
- 作者机构：
  
  400010 重庆，陆军特色医学中心肾内科
- 作者简介：
- 基金信息：
- DOI：10.16016/j.2097-0927.202202132
  CLC： R329.25;R363.272;R572.2
- Received：23 February 2021，
  
  Revised：2022-05-25，
  
  Published：30 November 2022
- 稿件说明：
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Xiaoyue WANG, Qingli CAI, Fang YU, et al. Exosomes derived from bone marrow mesenchymal stem cells ameliorate peritoneal fibrosis induced by high glucose peritoneal dialysate solution in mice[J]. Journal of Army Medical University, 2022, 44(22): 2291-2299.
DOI：

Xiaoyue WANG, Qingli CAI, Fang YU, et al. Exosomes derived from bone marrow mesenchymal stem cells ameliorate peritoneal fibrosis induced by high glucose peritoneal dialysate solution in mice[J]. Journal of Army Medical University, 2022, 44(22): 2291-2299. DOI： 10.16016/j.2097-0927.202202132.

摘要

目的

探讨骨髓间充质干细胞(bone marrow mesenchyml stem cell

BMSCs)来源的外泌体在高糖腹透液诱导的小鼠腹膜纤维化中的作用。

方法

选取32只6~8周龄的C57bl/6雄鼠，随机分为为4组(

=8)：正常对照组、生理盐水组、4.25%腹膜透析液组、4.25%腹膜透析液+外泌体组。建立高糖腹透液诱导的腹膜纤维化小鼠模型，42 d后处死小鼠并收集标本。通过改良腹膜平衡实验(peritoneal balance test

PET)检测小鼠腹膜功能；免疫组化法检测小鼠腹膜纤维化标志物的表达；实时定量PCR法检测小鼠腹膜纤维化因子mRNA的表达；ELISA法检测小鼠促纤维化因子的表达。

结果

4.25%腹膜透析液组与对照组小鼠比较，腹膜间皮下区域厚度明显增加(

0.01)；4.25%腹膜透析液组腹膜纤维化标志物(α-SMA、Collagen-Ⅰ、Collagen-Ⅲ)表达显著增强，而4.25%腹膜透析液+外泌体组的腹膜纤维化标志物表达显著低于4.25%腹膜透析液组(

0.01)。4.25%腹膜透析液+外泌体组小鼠相较4.25%腹膜透析液组的超滤功能显著改善(

0.05)。qRT-PCR结果显示，与4.25%腹膜透析液组比较，4.25%腹膜透析液+外泌体组腹膜促纤维化因子TGF-β1(转化生长因子-β1)水平明显降低(

0.05)。

结论

BMSCs来源的外泌体可有效减轻高糖腹透液诱导的小鼠腹膜纤维化。BMSCs来源的外泌体治疗可能成为抑制腹膜纤维化的新方法。

Abstract

Objective

To investigate the role of exosomes derived from bone marrow mesenchymal stem cells (BMSCs) in peritoneal fibrosis induced by high glucose peritoneal dialysis in mice.

Methods

Thirty-two male C57bl/6 mice were randomly divided into 4 groups: the normal control

the saline

the 4.25% peritoneal dialysate and the 4.25% peritoneal dialysate+exosome group. A mouse model of peritoneal fibrosis induced by high glucose peritoneal dialysis was established. The mice were sacrificed after 42 d and the specimens were collected. Peritoneal function was detected by modified peritoneal balance test (PET); The expression of peritoneal fibrosis markers was detected by immunohistochemical method; The expression of peritoneal fibrosis factor mRNA was detected by real-time quantitative PCR

and the expression of pro-fibrotic factor was detected by ELISA.

Results

Compared with the normal control group

the 4.25% peritoneal dialysis group had obviously increased thickness of sub

cutaneous area of peritoneal mesothelium (

0.01). The expression of peritoneal fibrosis markers (α-SMA

collagen-Ⅰ and Collagen-Ⅲ) was significantly increased in the 4.25% peritoneal dialysate group

while the expression of peritoneal fibrosis markers in the 4.25% peritoneal dialysate+exosome group was significantly lower than that in the 4.25% peritoneal dialysate group

0.01). Compared with the 4.25% peritoneal dialysate+exosome group

the ultrafiltration function of mice in the 4.25% peritoneal dialysate+exosome group was significantly improved (

0.05). qRT-PCR results showed that the level of peritoneal fibrosis promoting factor TGF-β1 in the 4.25% peritoneal dialysate+exosome group was significantly lower than that in the 4.25% peritoneal dialysate+exosome group (

0.05).

Conclusion

Exosomes derived from BMSCs can effectively reduce peritoneal fibrosis induced by high glucose peritoneal dialysis solution in mice. Exosomes derived from BMSCs may be a new method to inhibit peritoneal fibrosis.

关键词

Keywords

references

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