Characteristics of ventromedial thalamic neuronal activity during sleep-wake cycle in mice

Bin WANG; Yubo HU; Liuxia TONG; Jie ZHANG; Bo HU

doi:10.16016/j.2097-0927.202202028

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Characteristics of ventromedial thalamic neuronal activity during sleep-wake cycle in mice

Neurosciences | 更新时间：2026-01-09

- Characteristics of ventromedial thalamic neuronal activity during sleep-wake cycle in mice
- Journal of Army Medical University Vol. 44, Issue 16, Pages: 1585-1591(2022)
- 作者机构：
  
  1.400038 重庆，陆军军医大学(第三军医大学)基础医学院：生理学教研室
  2.400038 重庆，陆军军医大学(第三军医大学)基础医学院：大队15队
  3.400042 重庆，陆军特色医学中心骨科
- 作者简介：
- 基金信息：
  
  the General Program of Natural Science Foundation of Chognqing(CSTC2019jcyj-msxmX0424);the Undergraduates Scientific Research and Cultivation Project of Army Medical University(2020XBK09);the Undergraduates Scientific Research and Cultivation Project of Army Medical University(2021XBK45)
- DOI：10.16016/j.2097-0927.202202028
  CLC： R338.24;R338.63;R338.8
- Received：11 February 2022，
  
  Revised：2022-05-03，
  
  Published：30 August 2022
- 稿件说明：
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Bin WANG, Yubo HU, Liuxia TONG, et al. Characteristics of ventromedial thalamic neuronal activity during sleep-wake cycle in mice[J]. Journal of Army Medical University, 2022, 44(16): 1585-1591.
DOI：

Bin WANG, Yubo HU, Liuxia TONG, et al. Characteristics of ventromedial thalamic neuronal activity during sleep-wake cycle in mice[J]. Journal of Army Medical University, 2022, 44(16): 1585-1591. DOI： 10.16016/j.2097-0927.202202028.

摘要

目的

研究腹内侧丘脑(ventromedial thalamus，VM)神经元在觉醒-睡眠周期以及状态转换过程中的放电活动特征。

方法

利用顶叶皮层脑电活动和头颈部三维加速度对雄性小鼠(

=4)脑功能状态进行评判，将其脑功能状态分为觉醒(wakefulness)、非快速动眼(non-rapid eye movement，NREM)睡眠和快速动眼(rapid eye movement，REM)睡眠3种。用可推进的微电极阵列记录小鼠VM和腹外侧丘脑(ventrolateral thalamus，VL)神经元在3种脑功能状态及相互转换过程中的放电活动。

结果

① 小鼠VM神经元(

=50)在觉醒状态下的放电频率[(4.13±0.47) Hz

]

显著高于NREM睡眠[(2.46±0.34) Hz

0.01

]

与REM睡眠[(2.35±0.32) Hz，

0.01

]

状态，但其在NREM与REM睡眠状态下的放电频率差异无统计学意义(

0.05)。②在NREM睡眠向觉醒转换期间，VM神经元放电频率显著增加(

0.01)。在觉醒向NREM睡眠转换过程中，VM神经元放电频率显著降低(

0.01)。但在NREM睡眠向REM睡眠转换过程中，VM神经元放电频率无显著变化

(

0.05)。③VL神经元(

=40)在NREM睡眠期间的放电频率[(3.68±0.62) Hz

]

低于觉醒期[(10.42±1.03) Hz，

0.01

]

和REM睡眠期[(8.95±0.89) Hz，

0.01

]

。VL神经元在NREM睡眠向觉醒转换(

0.01)以及NREM睡眠向REM睡眠转换(

0.01)期间放电频率显著增加。

结论

小鼠VM神经元的放电活动在觉醒状态下最强。其活动在觉醒向睡眠转换时减弱，并在睡眠向觉醒状态转换时增强。

Abstract

Objective

To investigate the characteristics of ventromedial thalamus (VM) neuronal activity during sleep-wake cycles.

Methods

Both the oscillation state of electrical activity in the parietal cortex and three-dimensional acceleration of the head and neck were employed to determine the brain states of male mouse (

=4). The brain states were classified into three types: wakefulness

non-rapid eye movement (NREM) sleep and rapid eye movement (REM) sleep.

In vivo

multi-electrode arrays were utilized to record the firing activity of VM and ventrolateral thalamus (VL) neurons in these three distinctive brain states and during their switching process.

Results

① The firing rates of VM neurons (

=50) during wakefulness (4.13± 0.47) Hz were significantly higher than in either NREM sleep (2.46±0.34 Hz

0.01) or REM sleep (2.35±0.32 Hz

0.01). There was no significant difference as to the firing rates between NREM and REM sleep state (

0.05). ②The firing rates of VM neurons (

=50) increased significantly from NREM sleep state to wakefulness (

0.001) and evidently reduced from wakefulness to NREM sleep state (

0.01). However

there was no obvious difference from NREM sleep state to REM sleep state (

0.05). ③The firing rates of VL neurons (

=40) during NREM sleep st

ate (3.68±0.62) Hz were lower than in either wakefulness (10.42±1.03 Hz

0.01) or REM sleep condition (8.95±0.89 Hz

0.01). In contrast

the firing rates of VL neurons significantly increased from NREM sleep stated to either wakefulness (

0.01) or REM sleep state (

0.01).

Conclusion

The wakefulness state has the greatest firing activities of VM neurons. The firing activities of VM neurons reduce during the transition from wakefulness to sleep state

whereas the activities increase during the transition from sleep state to wakefulness state.

关键词

Keywords

references

LIU D Q, DAN Y. A motor theory of sleep-wake control: arousal-action circuit[J]. Annu Rev Neurosci, 2019, 42: 27-46. DOI: 10.1146/annurev-neuro-080317-061813.

GUOZ V, INAGAKI H K, DAIE K, et al . Maintenance of persistent activity in a frontal thalamocortical loop[J ] . Nature, 2017, 545(7653): 181-186. DOI: 10.1038/nature22324.

TAKAHASHI N, MOBERG S, ZOLNIKT A, et al . Thalamic input to motor cortex facilitates goal-directed action initiation[J ] . Curr Biol, 2021, 31(18): 4148-4155. e4. DOI: 10.1016/j.cub.2021.06.089.

KURAMOTO E, OHNO S, FURUTA T, et al . Ventral medial nucleus neu rons send thalamocortical afferents more widely and more preferentially to layer 1 than neurons of the ventral anterior-ventral lateral nuclear complex in the rat[J ] . Cereb Cortex, 2015, 25(1): 221-235. DOI: 10.1093/cercor/bht216.

GUO K H, YAMAWAKI N, SVOBODA K, et al . Anterolateral motor cortex connects with a medial subdivision of ventromedial thalamus through cell type-specific circuits, forming an excitatory thalamo-cortico-thalamic loop via layer 1 apical tuft dendrites of layer 5B pyramidal tract type neurons[J ] . J Neurosci, 2018, 38(41): 8787-8797. DOI: 10.1523/JNEUROSCI.1333-18.2018.

HONJOH S, SASAI S, SCHIERECKS S, et al . Regulation of cortical activity and arousal by the matrix cells of the ventromedial thalamic nucleus[J ] . Nat Commun, 2018, 9(1): 2100. DOI: 10.1038/s41467-018-04497-x.

KEBSCHULLJ M, RICHMAN E B, RINGACH N, et al . Cerebellar nuclei evolved by repeatedly duplicating a conserved cell-type set[J ] . Science, 2020, 370(6523): eabd5059. DOI: 10.1126/science.abd5059.

TANAKAY H, TANAKA Y R, KONDO M, et al . Thalamocortical axonal activity in motor cortex exhibits layer-specific dynamics during motor learning[J ] . Neuron, 2018, 100 (1): 244-258. e12. DOI: 10.1016/j.neuron.2018.08.016.

ZHANG J, CHEN H, ZHANGL B, et al . Ventromedial thalamus-projecting DCN neurons modulate associative sensorimotor responses in mice[J ] . Neurosci Bull, 2022: 2022Jan6. DOI: 10.1007/s12264-021-00810-9.

SCHÄFER C B, GAO Z Y, DE ZEEUW C I, et al . Temporal dynamics of the cerebello-cortical convergence in ventro-lateral motor thalamus[J ] . J Physiol, 2021, 599(7): 2055-2073. DOI: 10.1113/JP280455.

GAO Z Y, DAVIS C, THOMAS A M, et al . A cortico-cerebellar loop for motor planning[J ] . Nature, 2018, 563(7729): 113-116. DOI: 10.1038/s41586-018-0633-x.

GORNATIS V, SCHÄFER C B, EELKMAN ROODA O H J, et al . Differentiating cerebellar impact on thalamic nuclei[J ] . Cell Rep, 2018, 23(9): 2690-2704. DOI: 10.1016/j.celrep.2018.04.098.

DESSEILLES M, DANG-VU T, SCHABUS M, et al . Neuroimaging insights into the pathophysiology of sleep disorders[J ] . Sleep, 2008, 31(6): 777-794. DOI: 10.1093/sleep/31.6.777.

JOOE Y, NOH H J, KIM J S, et al . Brain gray matter deficits in patients with chronic primary insomnia[J ] . Sleep, 2013, 36(7): 999-1007. DOI: 10.5665/sleep.2796.

PEDROSOJ L, BRAGA-NETO P, ESCORCIO-BEZERRA M L, et al . Non-motor and extracerebellar features in spinocerebellar Ataxia type 2[J ] . Cerebellum, 2017, 16(1): 34-39. DOI: 10.1007/s12311-016-0761-5.

YUAN X Q, OU R W, HOU Y B, et al . Extra-cerebellar signs and non-motor features in Chinese patients with spinocerebellar Ataxia type 3[J ] . Front Neurol, 2019, 10: 110. DOI:10.3389/fneur.2019.00110.

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