Preparation of near-infrared light responsive molybdenum disulfide/sodium alginate nanocomposite gel and its hemostatic and antibacterial properties
- Journal of Army Medical University Vol. 44, Issue 8, Pages: 749-756(2022)
- 作者机构：
1.400037 重庆，陆军军医大学(第三军医大学)第二附属医院肝胆外科
2.400044 重庆，重庆大学能源与动力工程学院，重庆大学-新加坡国立大学新能源材料与器件联合实验室
- 作者简介：
- 基金信息：the National Natural Science Foundation of China(52173235);the Key Clinical Technology Innovation Project of the Second Affiliated Hospital of Army Medical University(2018JSLC00025)
DOI：10.16016/j.2097-0927.202201016
CLC： R318.08;R944.15;R973.1Received：04 January 2022，
Revised：2022-02-24，
Published：30 April 2022
- 稿件说明：
移动端阅览
Chao RAN, Jiacheng WANG, Meng LI, et al. Preparation of near-infrared light responsive molybdenum disulfide/sodium alginate nanocomposite gel and its hemostatic and antibacterial properties[J]. Journal of Army Medical University, 2022, 44(8): 749-756.
Chao RAN, Jiacheng WANG, Meng LI, et al. Preparation of near-infrared light responsive molybdenum disulfide/sodium alginate nanocomposite gel and its hemostatic and antibacterial properties[J]. Journal of Army Medical University, 2022, 44(8): 749-756. DOI： 10.16016/j.2097-0927.202201016.
摘要
目的
2
制备一种新型二硫化钼/海藻酸钠纳米复合凝胶，评价其微观结构、理化特性和止血抗菌性能。
方法
2
采用两步法制备二硫化钼/海藻酸钠纳米复合凝胶。通过水热法合成二硫化钼(molybdenum disulfide，MoS
2
)纳米片。制备2%海藻酸钠(sodium alginate，SA)溶液
然后以此为模板，添加不同浓度MoS
2
分散液，混合均匀后通过钙离子交联得到SA/MoS
2
-X凝胶；扫描电子显微镜观察SA、SA/MoS
2
-X微观结构；傅里叶红外光谱测试(FTIR)通过图谱上各种振
动峰的标定分析物质表面官能团；紫外可见光吸收光谱(UV-Vis)测试物质对特定波长的吸收等；光热抗菌实验评价SA/MoS
2
-X凝胶对大肠杆菌和金黄色葡萄球菌繁殖的抑制作用；大鼠肝脏出血模型评价SA/MoS
2
-X凝胶的体内止血效果。
结果
2
扫描电子显微镜结果显示，两步法合成的SA/MoS
2
-X凝胶的纤维网络呈三维立体多孔结构，其孔隙大小和密度与纯SA凝胶有区别；MoS
2
附着于SA凝胶表面，均匀分布；紫外可见光吸收光谱显示SA/MoS
2
-X比SA在808 nm处吸收峰值显著增加。SA/MoS
2
-X凝胶(分别含1 mg/mL和2 mg/mL MoS
2
)对大肠杆菌和金黄色葡萄球菌的光热抗菌效果显著高于SA组(
P
<
05)。SA/MoS
2
-X凝胶对SD大鼠肝脏出血模型的止血效果显著高于对照组(
P
<
05)。
结论
2
两步法制备的SA/MoS
2
-X凝胶具有良好的孔隙率和MoS
2
结合稳定性，其光热特性和理化性能得到进一步提高，且对大肠杆菌和金黄色葡萄球菌具有较好的抗菌活性，同时具有良好的止血效果。
Abstract
Objective
2
To prepare a new type of molybdenum disulfide (MoS
2
)/sodium alginate (SA) nanocomposite gel
and evaluate its microstructure
physical and chemical properties as well as its hemostatic and antibacterial performances.
Methods
2
The nanocomposite gel was prepared with a two-step method. Firstly
MoS
2
nanosheets were synthesized by hydrothermal method; secondly
2% SA solution was prepared as a template
being added with different concentrations of MoS
2
dispersion; and then the SA/MoS
2
-X gel was obtained by crosslinking with calcium ions after being mixed evenly. The microstructure of SA and SA/MoS
2
-X were observed by scanning electron microscopy (SEM). Fourier transform infrared spectrometer (FTIR) analysis was used to analyze the surface functional groups of the substance through calibrating various vibration peaks on the spectrum. The absorption of the substance to specific wavelengths were measured by ultraviolet-visible (UV-Vis) light absorption spectroscopy. The inhibitory effects against
E. coli
and
S. aureus
and the hemostatic effect of SA/MoS
2
-X gel were evaluated by photothermal antibacterial experiment and rat
liver hemorrhage model
respectively.
Results
2
The SEM results showed that the fiber network of the SA/MoS
2
-X gel was a 3-D porous structure
with its pore size and density being different from those of pure SA gel
and MoS
2
was attached to the surface of SA gel and evenly distributed. The UV-Vis absorption spectrum indicated that the absorption peak of SA/MoS
2
-X was significantly higher than that of SA at 808 nm. The photothermal antibacterial effects against
E.coli
and
S.aureus
were remarkably enhanced in the SA/MoS
2
-X gel group (containing 1 and 2 mg/mL MoS
2
respectively) (
P
<
0.05). In addition
the hemostatic effect of SA/MoS
2
-X gel on liver hemorrhage model of SD rats was greatly improved as compared with the control group (
P
<
0.05).
Conclusion
2
The SA/MoS
2
-X gel prepared by the two-step method has good porosity and binding stability to MoS
2
with further improved photothermal and physicochemical properties
and presents high antibacterial performance against
E.coli
and
S.aureus
as well as good hemostatic effectiveness.
关键词
Keywords
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