Stathmin mediates TNF-α-induced skin fibroblast migration by promoting formation of filopodia

Ruiyan CEN; Lingfei LI; Yumeng DENG; Yongqing HE; Liqun WANG; Chenda YUE; Xia LEI

doi:10.16016/j.2097-0927.202112129

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Stathmin mediates TNF-α-induced skin fibroblast migration by promoting formation of filopodia

Basic Medicine | 更新时间：2026-01-09

- Stathmin mediates TNF-α-induced skin fibroblast migration by promoting formation of filopodia
- Journal of Army Medical University Vol. 44, Issue 7, Pages: 630-638(2022)
- 作者机构：
  
  400042 重庆, 陆军特色医学中心皮肤科
- 作者简介：
- 基金信息：
  
  the General Program of National Natural Science Foundation of China(81773348);the National Natural Science Foundation for Young Scholars of China(82003323);the Project of Military Medical Frontier Innovation Program of Daping Hospital(2019CXJSC014)
- DOI：10.16016/j.2097-0927.202112129
  CLC： R322.99;R329.24;R363.22
- Received：15 December 2021，
  
  Revised：2022-01-24，
  
  Published：15 April 2022
- 稿件说明：
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Ruiyan CEN, Lingfei LI, Yumeng DENG, et al. Stathmin mediates TNF-α-induced skin fibroblast migration by promoting formation of filopodia[J]. Journal of Army Medical University, 2022, 44(7): 630-638.
DOI：

Ruiyan CEN, Lingfei LI, Yumeng DENG, et al. Stathmin mediates TNF-α-induced skin fibroblast migration by promoting formation of filopodia[J]. Journal of Army Medical University, 2022, 44(7): 630-638. DOI： 10.16016/j.2097-0927.202112129.

摘要

目的

探索stathmin在肿瘤坏死因子α(TNF-α)刺激模拟的创面愈合早期炎症反应中，对皮肤成纤维细胞迁移的作用及初步机制。

方法

TNF-α刺激人真皮成纤维细胞模拟体外创面愈合早期炎症反应，通过免疫荧光观察TNF-α对伪足生成的影响，细胞划痕实验观察迁移的改变，免疫荧光及Western blot观察TNF-α对stathmin表达的影响；细胞松弛素B刺激细胞和小干扰RNA(siSTMN)干扰stathmin表达后，通过免疫荧光观察伪足的改变，细胞划痕实验观察迁移的变化。

结果

TNF-α处理人真皮成纤维细胞后，伪足明显增加，并且细胞迁移显著增强(

0.05)，stathmin表达显著增加(

0.05

)；细胞松弛素B导致伪足生成减少，细胞迁移显著减弱(

0.05)；干扰stathmin表达后，细胞伪足减少，迁移能力显著减弱(

0.05)。

结论

在早期炎症中，stathmin可能通过促进伪足形成介导皮肤成纤维细胞的迁移。

Abstract

Objective

To explore the effect and preliminary mechanism of stathmin on skin fibroblast migration in the early inflammatory reaction of wound healing mimicked by TNF-α stimulation.

Methods

Human dermal fibroblast was stimulated by TNF-α to establish a cell model of inflammation at the early stage of wound healing. The effect of TNF-α on filopodium formation was observed by immunofluorescence assay

cell migration was detected by scratch wound healing

and stathmin expression level under TNF-α stimulation was measured by immunofluorescence assay and Western blotting. After cytochalasin B stimulation and small interfering RNA were used to interfere stathmin expression

respectively

immunofluorescence assay and scratch wound healing were performed again to observe the changes of filopodia and cell migration.

Results

TNF-α treatment promoted the formation of filopodia in human dermal fibroblasts

enhanced cell migration (

0.05) and increased stathmin expression (

0.05). Cytochalasin B treatment reduced the formation of filopodia and cell migration (

0.05); and interference of stathmin expression suppressed the formation of filopodia and reduced cell migration (

0.05).

Conclusion

In early inflammation

stathmin mediates skin fibroblast migration by promoting the formation of filopodia.

关键词

Keywords

references

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