Signal molecule autoinducer 2 promotes biofilm formation and virulence production in <italic style="font-style: italic">Pseudomonas aeruginosa</italic> by quorum sensing system

Hongdong LI; Jialin YU; Liping TAN; Qing AI; Xingyuan LI

doi:10.16016/j.2097-0927.202112073

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Signal molecule autoinducer 2 promotes biofilm formation and virulence production in Pseudomonas aeruginosa by quorum sensing system

Basic Medicine | 更新时间：2026-01-09

- Signal molecule autoinducer 2 promotes biofilm formation and virulence production in Pseudomonas aeruginosa by quorum sensing system
- Journal of Army Medical University Vol. 44, Issue 14, Pages: 1440-1444(2022)
- 作者机构：
  
  1.400014 重庆，重庆医科大学附属儿童医院：急诊科，国家儿童健康与疾病临床医学研究中心，儿童发育疾病研究教育部重点实验室，儿科学重庆市重点实验室
  2.400014 重庆，重庆医科大学附属儿童医院：新生儿研究室，国家儿童健康与疾病临床医学研究中心，儿童发育疾病研究教育部重点实验室，儿科学重庆市重点实验室
  3.400020 重庆，重庆市江北区人民医院药剂科
- 作者简介：
- 基金信息：
  
  the Natural Science Foundation of Chongqing(cstc2020jcyj-msxmX0253)
- DOI：10.16016/j.2097-0927.202112073
  CLC： R378.99;R394.2
- Received：09 December 2021，
  
  Revised：2022-04-01，
  
  Published：30 July 2022
- 稿件说明：
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Hongdong LI, Jialin YU, Liping TAN, et al. Signal molecule autoinducer 2 promotes biofilm formation and virulence production in Pseudomonas aeruginosa by quorum sensing system[J]. Journal of Army Medical University, 2022, 44(14): 1440-1444.
DOI：

Hongdong LI, Jialin YU, Liping TAN, et al. Signal molecule autoinducer 2 promotes biofilm formation and virulence production in Pseudomonas aeruginosa by quorum sensing system[J]. Journal of Army Medical University, 2022, 44(14): 1440-1444. DOI： 10.16016/j.2097-0927.202112073.

摘要

目的

研究信号分子AI-2对铜绿假单胞菌(

aeruginosa

)野生株(PAO1)及其密度感应(quorum sensing

QS)系统基因

lasR

rhlR

缺失株(

ΔlasRΔrhlR

)生物被膜(biofilm，BF)形成和毒力因子产生的影响。

方法

以PAO1和PAO1

LasR

rhlR

QS基因敲除菌株(

ΔLasR

rhlR

)建立生物被膜模型后分4组进行实验：单独PAO1组、AI-2+PAO1组、单独

ΔLasR

rhlR

组、AI-2+

ΔLasR

rhlR

组。结晶紫染色测量各组细菌生物被膜量；激光共聚焦显微镜观察BF形成；K-B纸片扩散法行药敏实验；氯仿萃取法、刚果红法分别测定不同组产生毒力因子绿脓菌素和弹性蛋白酶的量或者活性。

结果

AI-2的作用后，AI-2+PAO1组生物被膜形成、毒力因子的产生都显著高于单独PAO1组，抗生素抑菌圈直径显著小于单独PAO1组(

0.05)。

ΔLasR

rhlR

组生物被膜形成、毒力因子的产生都显著低于PAO1组，抗生素抑菌圈直径显著大于PAO1组(

0.05)。而单独

ΔLasR

rhlR

与AI-2+

ΔLasR

rhlR

两组相比，其生物被膜形成、毒力因子的产生和抗生素抑菌圈直径无明显差异(

0.05)。

结论

AI-2通过

QS系统表达促进其BF形成、毒力因子的产生。

Abstract

Objective

To investigate the effects of signal molecule autoinducer 2 (AI-2) on the biofilm formation and virulence production in the wild type strain

PAO1 and the strain with deficient quorum sensing genes

lasR

and

rhlR

(ΔlasRΔ

rhlR

) of

Pseudomonas

aeruginosa

(

Methods

The wild strain PAO1 and the

ΔLasR

rhlR

strain were used to establish biofilm model. The following experiments were performed on 4 groups

including PAO1 group

AI-2+PAO1 group

ΔLasR

rhlR

group and AI-2+

ΔLasR

rhlR

group. The biofilm formation was measured by crystal violet assay and observed with confocal laser scanning microscopy (CLSM). Kirby-Bauer disk diffusion was used for drug sensitivity test. Virulence factor pyocyanin and elastase production were measured by chloroform extraction method and Congo red assay

respectively.

Results

Compared to the wild type PAO1

the biofilm formation and virulence production were significantly increased in AI-2+PAO1 group

as well as the diameter of inhibition zone was significantly decreased(

0.05)

the biofilm formation and virulence production were significantly reduced in AI-2+

ΔLasR

rhlR

group

and the diameter of inhibition zone was significantly enlarged (

0.05). However

there were no significant differences between the

ΔLasR

rhlR

group and AI-2+

ΔLasR

rhlR

group in biofilm formation

virulence production and the diameter of inhibition zone (

0.05).

Conclusion

AI-2 enhances the biofilm formation and virulence production of

by the quorum sensing system.

关键词

Keywords

references

PAPAZIAN L, KLOMPAS M, LUYT C E. Ventilator-associated pneumonia in adults: a narrative review[J]. Intensive Care Med, 2020, 46(5): 888-906. DOI: 10.1007/s00134-020-05980-0.

FERNÁNDEZ-BARAT L, TORRES A. Biofilms in ventilator-associatedpneumonia[J]. Future Microbiol, 2016, 11: 1599-1610. DOI: 10.2217/fmb-2016-0040.

DEXTER A M, SCOTT J B. Airway management and ventilator-associatedevents[J]. Respir Care, 2019, 64(8): 986-993. DOI: 10.4187/respcare.07107.

RABIN N, ZHENG Y, OPOKU-TEMENG C, et al . Biofilm formation mechanisms and targets for developing antibiofilm agents[J ] . Future Med Chem, 2015, 7(4): 493-512. DOI: 10.4155/fmc.15.6.

LI H D, SONG C, LIU D, et al . Molecular analysis of biofilms on the surface of neonatal endotracheal tubes based on 16SrRNA PCR-DGGE and species-specific PCR[J ] . Int J Clin Exp Med, 2015, 8(7): 11075-11084.

WANG Y, LIU BB, GRENIER D, et al . Regulatory mechanisms of the LuxS/AI-2 system and bacterial resistance[J ] . Antimicrob Agents Chemother, 2019, 63(10): e01186-e01119. DOI: 10.1128/AAC.01186-19.

LI H D, LI X Y, WANG Z L, et al . Autoinducer-2 regulates Pseudomonas aeruginosa PAO1 biofilm formation and virulence production in a dose-dependent manner[J ] . BMC Microbiol, 2015, 15: 192. DOI: 10.1186/s12866-015-0529-y.

WANG Z L, XIANG QQ, YANG T, et al . Autoinducer-2 of Streptococcus mitis as a target molecule to inhibit pathogenic multi-species biofilm formation in vitro and in an endotracheal intubation rat model[J ] . Front Microbiol, 2016, 7: 88. DOI: 10.3389/fmicb.2016.00088.

DUAN K M, DAMMEL C, STEIN J, et al . Modulation of Pseudomonas aeruginosa gene expression by host microflora through inter species communication[J ] . Mol Microbiol, 2003, 50(5): 1477-1491. DOI: 10.1046/j.1365-2958.2003.03803.x.

傅雅琨, 余加林, 芦起, 等. 铜绿假单胞菌与轻型链球菌混合生物膜体外模型的建立[J]. 中国抗生素杂志, 2015, 40(4): 278-284. DOI: 10.13461/j.cnki.cja.005528.

FU Y K, YU J L, LU Q, et al . Establishment of in vitro mixed-biofilm model formed by Pseudomonas aeruginosa and Streptococcus mitis[J ] . Chin J Antibiot, 2015, 40(4): 278-284. DOI: 10.13461/j.cnki.cja.005528..

FAZLI M, ALMBLAD H, RYBTKE M L, et al . Regulation of biofilm formation in Pseudomonas and Burkholderia species[J ] . Environ Microbiol, 2014, 16(7): 1961-1981. DOI: 10.1111/1462-2920.12448.

HOLM A, VIKSTRÖM E. Quorum sensing communication between bacteria and human cells: signals, targets, andfunctions[J]. Front Plant Sci, 2014, 5: 309. DOI: 10.3389/fpls.2014.00309.

KIM C, KIM J, PARK H Y, et al . Structural understanding of quorum-sensing inhibitors by molecular modeling study in Pseudomonas aeruginosa[J ] . Appl Microbiol Biotechnol, 2009, 83(6): 1095-1103. DOI: 10.1007/s00253-009-1954-3.

PENA R T, BLASCO L, AMBROA A, et al . Relationship between quorum sensing and secretionsystems[J ] . Front Microbiol, 2019, 10: 1100. DOI: 10.3389/fmicb.2019.01100.

BACHTIAR E W, BACHTIAR B M, JAROSZ L M, et al . AI-2 of Aggregatibacter actinomycetemcomitans inhibits Candida albicans biofilm formation[J ] . Front Cell Infect Microbiol, 2014, 4: 94. DOI: 10.3389/fcimb.2014.00094.

LEE N R, KIM M D, LIM M C. Autoinducer-2 could affect biofilm formation by food-derived Bacilluscereus[J]. Indian J Microbiol, 2021, 61(1): 66-73. DOI: 10.1007/s12088-020-00918-y.

KARIM MM, HISAMOTO T, MATSUNAGA T, et al . LuxS affects biofilm maturation and detachment of the periodontopathogeni c bacterium Eikenella corrodens[J ] . J Biosci Bioeng, 2013, 116(3): 313-318. DOI: 10.1016/j.jbiosc.2013.03.013.

YADAV M K, VIDAL J E, GO YY, et al . The LuxS/AI-2 quorum-sensing system of Streptococcus pneumoniae is required to cause disease, and to regulate virulence- and metabolism-related genes in a rat model of middle ear infection[J ] . Front Cell Infect Microbiol, 2018, 8: 138. DOI: 10.3389/fcimb.2018.00138.

SUN Y C, LI Y, LUO Q, et al . LuxS/AI-2 quorum sensing system in Edwardsiella piscicida promotes biofilm formation and pathogenicity[J ] . Infect Immun, 2020, 88(5): e00907-e00919. DOI: 10.1128/IAI.00907-19.

罗哲, 王敏, 杜鸿, 等. LuxS/AI-2对伤寒沙门菌基因表达的调节[J]. 江苏大学学报(医学版), 2011, 21(1): 34-41. DOI: 10.13312/j.issn.1671-7783.2011.01.005.

LUO Z, WANG M, DU H, et al . Analysis of genes expression regulation controlled byluxS/AI-2 in Salmonella enterica serovar Typhi[J ] . J Jiangsu Univ Med Ed, 2011, 21(1): 34-41. DOI: 10.13312/j.issn.1671-7783.2011.01.005.

GEIER H, MOSTOWY S, CANGELOSI G A, et al . Autoinducer-2 triggers the oxidative stress response in Mycobacteriumavium, leading to biofilm formation[J ] . Appl Environ Microbiol, 2008, 74(6): 1798-1804. DOI: 10.1128/AEM.02066-07.

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⁰

Signal molecule autoinducer 2 promotes biofilm formation and virulence production in Pseudomonas aeruginosa by quorum sensing system

DOI：10.16016/j.2097-0927.202112073

摘要

Abstract

关键词

Keywords

references