Expression and clinical significance of H3K27me3 in hemangioma and vascular malformation

Junlan WANG; Yupeng CHEN; Sheng ZHANG; Linying CHEN; Qian HUANG; Zhenzhen ZHANG

doi:10.16016/j.2097-0927.202111216

您当前的位置：

首页 >

文章列表页 >

Expression and clinical significance of H3K27me3 in hemangioma and vascular malformation

Clinical Medicine | 更新时间：2026-01-09

- Expression and clinical significance of H3K27me3 in hemangioma and vascular malformation
- Journal of Army Medical University Vol. 44, Issue 13, Pages: 1356-1361(2022)
- 作者机构：
  
  350004 福州，福建医科大学附属第一医院病理科
- 作者简介：
- 基金信息：
  
  the Middle-Aged and Young Backbone Talent Cultivation Program for Fujian Health Industries(2019-ZQN-55);the Natural Science Foundation of Fujian Province(2020J01988)
- DOI：10.16016/j.2097-0927.202111216
  CLC： R341;R730.43;R732.2
- Received：30 November 2021，
  
  Revised：2022-01-15，
  
  Published：15 July 2022
- 稿件说明：
移动端阅览
Junlan WANG, Yupeng CHEN, Sheng ZHANG, et al. Expression and clinical significance of H3K27me3 in hemangioma and vascular malformation[J]. Journal of Army Medical University, 2022, 44(13): 1356-1361.
DOI：

Junlan WANG, Yupeng CHEN, Sheng ZHANG, et al. Expression and clinical significance of H3K27me3 in hemangioma and vascular malformation[J]. Journal of Army Medical University, 2022, 44(13): 1356-1361. DOI： 10.16016/j.2097-0927.202111216.

摘要

目的

探讨内皮细胞H3K27me3在血管瘤和血管畸形中的表达及临床意义。

方法

收集2010年1月至2021年6月福建医科大学附属第一医院病理科244例血管瘤和血管畸形患者库存蜡块，采用免疫组化SP法检测H3K27me3表达水平，分析H3K27me3在血管瘤和血管畸形中的表达差异，通过生物信息学分析并结合血管周细胞SMA染色的结果探讨其在血管异常发育中的潜在作用，应用ROC曲线评价其在血管瘤和血管畸形鉴别中的诊断价值。

结果

H3K27me3主要在血管瘤的内皮细胞表达，其在血管瘤的平均表达率为50%~90%，但血管畸形内皮细胞H3K27me3表达率为30%~50%，血管周细胞几乎不表达。基于GSE21212数据集并结合文献获得内皮细胞限制性表达基因10个，通过ENCODE网页工具分析发现不同谱系内皮细胞SOX2启动子区有H3K27me3富集，H3K27me3与SOX2交互作用可能与血管的发育和分化有关。

结论

内皮细胞H3K27me3可以作为血管瘤与血管畸形鉴别的补充标记物，但其在婴儿的血管瘤中表达具有异质性。

Abstract

Objective

To investigate the expression and clinical significance of H3K27me3 in vascular hemangioma and malformation.

Methods

Paraffin-embedded tissue blocks from 244 patients with hemangioma and vascular malformation admitted in our hospital from January 2010 and June 2021 were collected. The expression levels of H3K27me3 and smooth muscle actin (SMA) in vascular endothelial and perivascular cells were detected by immunohistochemical SP assay

and its expression differences between hemangioma and vascular malformation was analyzed. Bioinformatics analysis was further applied to explore its potential role in anigodysplasia in combination of the results of SMA immunostaining. Receiver operating characteristic (ROC) curve was drawn to evaluate its diagnostic value in differentiation of hemangioma and vascular malformation.

Results

In vascular hemangioma

H3K27me3 expression was mainly restricted to vascular endothelial cells

with an expression rate of 50%~90%. However

in vascular malformations

it was expressed in vascular endothelial cells

with an expression rate of 30%~50%

and lost in perivascular cells. There were top 10 endothelium restricted genes derived from GSE21212 dataset based on concerning literatures. According to ChIP-seq data from ENCODE website

H3K27me3 was enriched in the SOX2 promoter of different endothelium lineages

and the molecular interaction of H3K27me3 and SOX2 may be associated with vessel development and differentiation.

Conclusion

The expression of H3K27me3 in vascular endothelial cells can be used as a candidate marker to distinguish hemangioma and vascular malformation. However

its expression in infantile hemangioma is immunologically heterogeneous.

关键词

Keywords

references

何毅辉, 黄海建, 陈志忠, 等. 肝先天性血管瘤3例临床病理分析[J]. 临床与实验病理学杂志, 2021, 37(2): 224-226. DOI: 10.13315/j.cnki.cjcep.2021.02.023.

HE Y H, HUANG H J, CHEN Z Z, et al . Clinicopathological analysis of 3 cases of hepatic congenital hemangiomas[J ] . Chin J Clin Exp Pathol, 2021, 37(2): 224-226. DOI: 10.13315/j.cnki.cjcep.2021.02.023.

CHENG Z J, NAGA SRIKANTH GARIKIPATI V, TRUONGCAO M M, et al . Serum-derived small extracellular vesicles from diabetic mice impair angiogenic property of microvascular endothelial cells: role of EZH2[J ] . J Am Heart Assoc, 2021, 10(10): e019755. DOI: 10.1161/JAHA.120.019755.

CHENG X Y, WANG Y, DU L Z. Epigenetic modulation in the initiation and progression of pulmonary hypertension[J]. Hypertension, 2019, 74(4): 733-739. DOI: 10.1161/HYPERTENSIONAHA.119.13458.

中华医学会病理学分会儿科病理学组. 儿童脉管性病变诊断共识[J]. 中华病理学杂志, 2020, 49(3): 220-227.

Subspecialty Group of Paediatrics of Society of Pathology of Chinese Medical Association. Expert consensus on the diagnosis of vasculitides in children[J]. Chin J Pathol, 2020, 49(3): 220-227.

DI CROCE L, HELIN K. Transcriptional regulation by Polycomb group proteins[J]. Nat Struct Mol Biol, 2013, 20(10): 1147-1155. DOI: 10.1038/nsmb.2669.

PRICKAERTS P, ADRIAENS M E, BEUCKEN T V D, et al . Hypoxia increases genome-wide bivalent epigenetic marking by specific gain of H3K27me3[J ] . Epigenetics Chromatin, 2016, 9: 46. DOI: 10.1186/s13072-016-0086-0.

WIERDA R J, RIETVELD I M, VAN EGGERMOND M C, et al . Global histone H3 lysine 27 triple methylation levels are reduced in vessels with advanced atherosclerotic plaques[J ] . Life Sci, 2015, 129: 3-9. DOI: 10.1016/j.lfs.2014.10.010.

LINO CARDENAS C L, KESSINGER C W, MACDONALD C, et al . Inhibition of the methyltranferase EZH2 improves aortic performance in experimental thoracic aortic aneurysm[J ] . JCI Insight, 2018, 3(5): e97493. DOI: 10.1172/jci.insight.97493.

HULSHOFF M S, XU X B, KRENNING G, et al . Epigenetic regulation of endothelial-to-mesenchymal transition in chronic heart disease[J ] . Arterioscler Thromb Vasc Biol, 2018, 38(9): 1986-1996. DOI: 10.1161/ATVBAHA.118.311276.

VASTENHOUW N L, SCHIER A F. Bivalent histone modifications in early embryogenesis[J]. Curr Opin Cell Biol, 2012, 24(3): 374-386. DOI: 10.1016/j.ceb.2012.03.009.

FRAINEAU S, PALII C G, MCNEILL B, et al . Epigenetic activation of pro-angiogenic signaling pathways in human endothelial progenitors increases vasculogenesis[J ] . Stem Cell Rep, 2017, 9(5): 1573-1587. DOI: 10.1016/j.stemcr.2017.09.009.

CHAI M M, GU C, SHEN Q H, et al . Hypoxia alleviates dexamethasone-induced inhibition of angiogenesis in cocultures of HUVECs and rBMSCs via HIF-1α[J ] . Stem Cell Res Ther, 2020, 11: 343. DOI:10.1186/s13287-020-01853-x..

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Remodeling characteristics of H3K27me3-marked silencers in gastric signet-ring cell carcinoma and its transcriptional regulatory function

Loss of histone H3K27me3 up-regulates SLC7A11 in diffuse gastric cancer cells

Construction and analysis of lncRNA-related ceRNA network in mouse model of methotrexate-induced neural tube defect under folate deficiency

Dihydroartemisinin induces pyroptosis and regulates apoptosis-associated speck-like protein methylation in prostate cancer cells

Clinical significance of BCL2L10 expression and promoter methylation in myelodysplastic syndrome

Related Author

DU Aibei

REN Yuanfeng

CHU Zhaole

LIU Biying

LI Xianfeng

XIANG Junyu

CHEN Dongfeng

WANG Tao

Related Institution

Department of Cell Biology， College of Basic Medical Sciences， Army Medical University （Third Military Medical University）

Department of Gastroenterology， Chongqing Key Laboratory of Precise Prevention and Treatment of Digestive Malignancies， Army Medical Center of PLA/Daping Hospital of Third Military Medical University

Department of Oncology， Chongqing Hospital of Traditional Chinese Medicine

Department of Gastroenterology， Chongqing Key Laboratory of Precision Diagnosis and Treatment for Digestive Malignancies， Army Medical Center of PLA/Daping Hospital of Third Military Medical University

Institue of Life Sciences, Chongqing Medical University

⁰