Association of ADAP2 gene with prognosis and immune infiltration in tumor microenvironment in patients with lung squamous cell carcinoma

Zheng CHEN; Ying XIANG; Na WU; Tingting XIA; Yifan SHAN; Weijia XIE; Bin XU; Zhiquan YUAN; Qin HU; Xiaoyue JIA; Long WU; Yafei LI

doi:10.16016/j.2097-0927.202110066

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Association of ADAP2 gene with prognosis and immune infiltration in tumor microenvironment in patients with lung squamous cell carcinoma

Basic Medicine | 更新时间：2026-01-09

- Association of ADAP2 gene with prognosis and immune infiltration in tumor microenvironment in patients with lung squamous cell carcinoma
- Journal of Army Medical University Vol. 44, Issue 4, Pages: 337-345(2022)
- 作者机构：
  
  400038 重庆，陆军军医大学(第三军医大学)军事预防医学系军队流行病学教研室
- 作者简介：
- 基金信息：
  
  the General Program of National Natural Science Foundation of China(81871896)
- DOI：10.16016/j.2097-0927.202110066
  CLC： R394.3;R730.7;R734.2
- Received：13 October 2021，
  
  Revised：2021-12-01，
  
  Published：28 February 2022
- 稿件说明：
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Zheng CHEN, Ying XIANG, Na WU, et al. Association of ADAP2 gene with prognosis and immune infiltration in tumor microenvironment in patients with lung squamous cell carcinoma[J]. Journal of Army Medical University, 2022, 44(4): 337-345.
DOI：

Zheng CHEN, Ying XIANG, Na WU, et al. Association of ADAP2 gene with prognosis and immune infiltration in tumor microenvironment in patients with lung squamous cell carcinoma[J]. Journal of Army Medical University, 2022, 44(4): 337-345. DOI： 10.16016/j.2097-0927.202110066.

摘要

目的

分析ADAP2基因与肺鳞癌患者预后及其与肿瘤微环境免疫细胞浸润的关联。

方法

下载TCGA(The Cancer Genome Atlas)数据库肺鳞癌患者临床信息和癌组织ADAP2基因转录水平，运用KM生存曲线和Cox比例风险模型分析ADAP2基因和患者预后的关联；采用xCell数据库分析ADAP2基因表达和肿瘤微环境免疫浸润的关系；利用免疫荧光双染实验观察ADAP2蛋白与M2型巨噬细胞标志物CD163的空间共定位。

结果

多因素Cox分析结果显示，ADAP2表达是肺鳞癌患者独立的预后因素，其高表达与肺鳞癌患者不良预后密切相关(

=1.533，95%

: 1.139~2.064，

=0.005)。ADAP2基因与肿瘤微环境评分呈正相关(

=0.609，95%

: 0.550~0.661，

＜0.001)，与免疫评分也同样呈正相关(

=0.596，95%

: 0.536~0.650，

＜0.001)。ADAP2基因与肺鳞癌单核吞噬细胞系统(巨噬细胞、巨噬细胞M1、巨噬细胞M2、单核细胞)的浸润呈高度正相关(

=0.737、0.718、0.603、0.631，

＜0.001)。ADAP2与CD163存在共定位，主要表达于细胞膜。

结论

ADAP2有望成为预测肺鳞癌患者预后和免疫治疗效果的潜在生物标志物。

Abstract

Objective

To investigate the correlation between ADAP2 gene and immune infiltration in tumor microenvironment in lung squamous cell carcinoma (LUSC)

and to explore the prognostic values of ADAP2 in patients with LUSC.

Methods

The clinical information of patients with LUSC as well as the data of ADAP2 mRNA levels in cancer tissues were downloaded from The Cancer Genome Atlas (TCGA) database. The association between ADAP2 gene and prognosis of patients was subsequently analyzed using Kaplan-Meier survival curve and Cox risk model analyses. xCell database was adopted to analyze the correlation of ADAP2 expression with immune infiltration in tumor microenvironment. Immunofluorescence double-staining test was employed to observe the co-localization of ADAP2 protein and CD163 (a specific biomarker of M2 macrophage).

Results

Multivariate Cox analysis showed that the expression of ADAP2 was an independent prognostic factor for LUSC patients

and its high expression was closely associated with the poor prognosis of patients (

=1.533

95%

: 1.139~2.064

=0.005). xCell analysis indicated that ADAP2 gene was positively correlated with both the tumor microenvironment score (

=0.609

95%

: 0.550~0.661

0.001)

and the immune infiltration score (

=0.596

95%

: 0.536~0.650

0.001)

and the expression of ADAP2 also showed a high positive association with mononuclear phagocyte system (macrophage

macrophage M1

macrophage M2 and monocyte) in LUSC (

=0.737

0.718

0.603 and 0.631

respectively

0.001). Moreover

ADAP2 presented co-localization with CD163 and was mainly expressed in the cell membrane.

Conclusion

ADAP2 can act as a potential biomarker for predicting prognosis and evaluating the efficacy of immunotherapy in LUSC.

关键词

Keywords

references

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