Mechanism of miR-409-3p negatively regulating epithelial mesenchymal transition of ovarian serous carcinoma HO-8910 cells by targeting ZEB1

Jing ZHU; Longhui ZHANG; Rongkai XIE

doi:10.16016/j.2097-0927.202109167

您当前的位置：

首页 >

文章列表页 >

Mechanism of miR-409-3p negatively regulating epithelial mesenchymal transition of ovarian serous carcinoma HO-8910 cells by targeting ZEB1

Basic Medicine | 更新时间：2026-01-09

- Mechanism of miR-409-3p negatively regulating epithelial mesenchymal transition of ovarian serous carcinoma HO-8910 cells by targeting ZEB1
- Journal of Army Medical University Vol. 44, Issue 5, Pages: 455-460(2022)
- 作者机构：
  
  400037 重庆，陆军军医大学(第三军医大学)第二附属医院妇产科
- 作者简介：
- 基金信息：
- DOI：10.16016/j.2097-0927.202109167
  CLC： R394-33;R73-362;R737.31
- Received：22 September 2021，
  
  Revised：2021-12-09，
  
  Published：15 March 2022
- 稿件说明：
移动端阅览
Jing ZHU, Longhui ZHANG, Rongkai XIE. Mechanism of miR-409-3p negatively regulating epithelial mesenchymal transition of ovarian serous carcinoma HO-8910 cells by targeting ZEB1[J]. Journal of Army Medical University, 2022, 44(5): 455-460.
DOI：

Jing ZHU, Longhui ZHANG, Rongkai XIE. Mechanism of miR-409-3p negatively regulating epithelial mesenchymal transition of ovarian serous carcinoma HO-8910 cells by targeting ZEB1[J]. Journal of Army Medical University, 2022, 44(5): 455-460. DOI： 10.16016/j.2097-0927.202109167.

摘要

目的

探讨miR-409-3p靶向ZEB1对卵巢浆液性癌HO-8910细胞上皮间质转化的影响及作用机制。

方法

利用脂质体分别将对照mimics和miR-409-3p mimics转染入HO-8910细胞中，qRT-PCR检测其转染效率，Western blot检测各组细胞中ZEB1、E-cadherin、N-cadherin和Vimentin蛋白表达水平，双荧光素酶报告基因实验检测miR-409-3p与ZEB1之间的靶向调控作用，Transwell侵袭和迁移实验分别各组细胞的侵袭和迁移能力变化。

结果

与mimics对照组相比，miR-409-3p过表达组中miR-409-3p表达显著升高(

0.05)，E-cadherin蛋白表达显著增加(

0.05)，ZEB1、N-cadherin和Vimentin蛋白表达明显减少(

0.05)，上皮间质转化进程被阻断。双荧光素酶报告基因实验结果显示ZEB1是miR-409-3p的直接作用靶点，过表达ZEB1可逆转转染miR-409-3p mimics介导的对细胞侵袭、迁移及上皮间质转化的抑制作用(

0.05)。

结论

过表达miR-409-3p通过抑制ZEB1蛋白表达，负性调控卵巢浆液性癌HO-8910细胞上皮间质转化，抑制肿瘤细胞的侵袭和转移。

Abstract

Objective

To investigate the effects of miR-409-3p on epithelial mesenchymal transition (EMT) of ovarian serous carcinoma HO-8910 cells by targeting ZEB1 and its underlying mechanism.

Methods

Control mimics and miR-409-3p mimics were transfected into HO-8910 cells by liposomes respectively

and qRT-PCR was adopted to detect the transfection efficiency. The expression of ZEB1

E-cadherin

N-cadherin

and Vimentin proteins in each cell group were examined by Western blotting

and the targeted regulation of ZEB1 by miR-409-3p was determined by dual luciferase reporter assay. Transwell invasion and migration assay was performed to observe the changes of invasion and migration abilities of the cells in each group.

Results

As compared with the control group

the expression of miR-409-3p was increased significantly in the miR-409-3p overexpression group (

0.05)

along with the elevation of E-cadherin expression (

0.05)

while the levels of ZEB1

N-cadherin and Vimentin were decreased remarkably (

0.05)

suggesting that the EMT process of HO-8910 cells was blocked. Furthermore

the results of dual luciferase reporter assay showed that ZEB1 was the direct target of miR-409-3p; overexpression of ZEB1 reversed the miR-409-3p mimics mediated repression of cell invasion

migration as well as EMT process (

0.05).

Conclusion

Overexpression of miR-409-3p negatively regulates the EMT process by inhibiting ZEB1 expression

and thus suppresses the invasion and metastasis of ovarian serous carcinoma HO-8910 cells.

关键词

Keywords

references

LISIO M A, FU L L, GOYENECHE A, et al . High-grade serous ovarian cancer: basic sciences, clinical and therapeutic standpoints[J ] . Int J Mol Sci, 2019, 20(4): 952. DOI: 10.3390/ijms20040952.

LU T X, ROTHENBERG M E. MicroRNA[J]. J Allergy Clin Immunol, 2018, 141(4): 1202-1207. DOI: 10.1016/j.jaci.2017.08.034.

LEE Y S, DUTTA A. MicroRNAs in cancer[J]. Annu Rev Pathol, 2009, 4: 199-227. DOI: 10.1146/annurev.pathol.4.110807.092222.

MISHRA S, YADAV T, RANI V. Exploring miRNA based approaches in cancer diagnostics and therapeutics[J]. Crit Rev Oncol Hematol, 2016, 98: 12-23. DOI: 10.1016/j.critrevonc.2015.10.003.

LIAN J, LIN S H, YE Y, et al . Serum microRNAs as predictors of risk for non-muscle invasive bladder cancer[J ] . Oncotarget, 2018, 9(19): 14895-14908. DOI: 10.18632/oncotarget.24473.

VENKATADRI R, MUNI T, IYER A K, et al . Role of apoptosis-related miRNAs in resveratrol-induced breast cancer cell death[J ] . Cell Death Dis, 2016, 7: e2104. DOI: 10.1038/cddis.2016.6.

ZHOU X, WEN W, SHAN X, et al . A six-microRNA panel in plasma was identified as a potential biomarker for lung adenocarcinoma diagnosis[J ] . Oncotarget, 2017, 8(4): 6513-6525. DOI: 10.18632/oncotarget.14311.

CAO Y D, ZHANG L, WEI M H, et al . MicroRNA-409-3p represses glioma cell invasion and proliferation by targeting high-mobility group nucleosome-binding domain 5[J ] . Oncol Res, 2017, 25(7): 1097-1107. DOI: 10.3727/096504017X14836170586829.

朱静, 张龙辉, 谢荣凯. miR-409-3p在卵巢浆液性癌中的表达及对HO-8910细胞生物学特性的影响[J]. 第三军医大学学报, 2020, 42(22): 2244-2250. DOI: 10.16016/j.1000-5404.202007047.

ZHU J, ZHANG L H, XIE R K. Expression of miR-409-3p in ovarian serous carcinoma and its effect on biological behaviors of HO-8910 cells[J]. J Third Mil Med Univ, 2020, 42(22): 2244-2250. DOI: 10.16016/j.1000-5404.202007047.

BRAGA E A, FRIDMAN M V, KUSHLINSKⅡ N E. Molecular mechanisms of ovarian carcinoma metastasis: key genes and regulatory microRNAs[J]. Biochemistry (Mosc), 2017, 82(5): 529-541. DOI: 10.1134/s0006297917050017.

PASTUSHENKO I, BLANPAIN C. EMT transition states during tumor progression and metastasis[J]. Trends Cell Biol, 2019, 29(3): 212-226. DOI: 10.1016/j.tcb.2018.12.001.

WANG D, LIU Z, YAN Z , et al . MiRNA-155-5p inhibits epithelium-to-mesenchymal transition (EMT) by targeting GSK-3β during radiation-induced pulmonary fibrosis[J ] . Arch Biochem Biophys, 2021, 697: 108699. DOI: 10.1016/j.abb.2020.108699.

ZHANG L Y, CHEN Y, JIA J, et al . MiR-27a promotes EMT in ovarian cancer through active Wnt/β-catenin signalling by targeting FOXO1[J ] . Cancer Biomark,2019, 24(1): 31-42. DOI: 10.3233/cbm-181229.

LAMOUILLE S, XU J, DERYNCK R. Molecular mechanisms of epithelial-mesenchymal transition[J]. Nat Rev Mol Cell Biol, 2014, 15(3): 178-196. DOI: 10.1038/nrm3758.

CARAMEL J, LIGIER M, PUISIEUX A. Pleiotropic roles for ZEB1 in cancer[J]. Cancer Res, 2018, 78(1): 30-35. DOI: 10.1158/0008-5472.can-17-2476.

WEI L, HE Y, BI S, et al . miRNA-199b-3p suppresses growth and progression of ovarian cancer via the CHK 1 /E-cadherin/EMT signaling pathway by targeting ZEB1[J ] . Oncol Rep, 2021, 45(2): 569-581. DOI: 10.3892/or.2020.7895..

GUO F, COGDELL D, HU L, et al . MiR-101 suppresses the epithelial-to-mesenchymal transition by targeting ZEB1 and ZEB2 in ovarian carcinoma[J ] . Oncol Rep, 2014, 31(5): 2021-2028. DOI: 10.3892/or.2014.3106.

WANG Y, YAN S, LIU X, et al . miR-1236-3p represses the cell migration and invasion abilities by targeting ZEB1 in high-grade serous ovarian carcinoma[J ] . Oncol Rep, 2014, 31(4): 1905-1910. DOI: 10.3892/or.2014.3046.

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

miR-576-5p regulates the EMT process to inhibit migration and invasion of hepatocellular carcinoma cells

Respiratory syncytial virus promotes apoptosis and expression of inflammatory factors in bronchial epithelial cells by regulating miR-409-3p/SIRT1 pathway

Role and mechanism of Wnt5a regulating proliferation and epithelial mesenchymal transition in A549 spheres

Related Author

崔琦

王余彦

卢映君

曾爱中

梅娟娟

田曼

严莎莎

甘聪

Related Institution

No data

⁰