Dihydromyricetin induces mitophagy by regulating Prohibitin 2 pathway in vascular endothelial cells

Qin HU; Hui SHEN; Ting ZHANG; Yafei LI

doi:10.16016/j.2097-0927.202107159

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Dihydromyricetin induces mitophagy by regulating Prohibitin 2 pathway in vascular endothelial cells

Basic Medicine | 更新时间：2026-01-09

- Dihydromyricetin induces mitophagy by regulating Prohibitin 2 pathway in vascular endothelial cells
- Journal of Army Medical University Vol. 44, Issue 4, Pages: 356-362(2022)
- 作者机构：
  
  1.400038 重庆，陆军军医大学(第三军医大学)军事预防医学系: 军队流行病学教研室
  2.400038 重庆，陆军军医大学(第三军医大学)军事预防医学系: 营养与食品卫生学教研室
- 作者简介：
- 基金信息：
  
  the National Natural Science Foundation for Young Scholars of China(81803229);the General Program of Natural Science Foundation of Chongqing(cstc2020jcyj-msxmX0105)
- DOI：10.16016/j.2097-0927.202107159
  CLC： R285.5;R322.12;R329.25
- Received：26 July 2021，
  
  Revised：2021-11-04，
  
  Published：28 February 2022
- 稿件说明：
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Qin HU, Hui SHEN, Ting ZHANG, et al. Dihydromyricetin induces mitophagy by regulating Prohibitin 2 pathway in vascular endothelial cells[J]. Journal of Army Medical University, 2022, 44(4): 356-362.
DOI：

Qin HU, Hui SHEN, Ting ZHANG, et al. Dihydromyricetin induces mitophagy by regulating Prohibitin 2 pathway in vascular endothelial cells[J]. Journal of Army Medical University, 2022, 44(4): 356-362. DOI： 10.16016/j.2097-0927.202107159.

摘要

目的

观察二氢杨梅素对血管内皮细胞线粒体自噬的影响，并探讨Prohibitin 2在其中的作用。

方法

用不同浓度(0、0.1、1、10、100 μmol/L)二氢杨梅素处理人脐静脉血管内皮细胞(human umbilical vein endothelial cells，HUVECs)12 h后，流式细胞术和荧光显微镜观测MitoTracker

Deep Red (MTDR)荧光强度，RT-PCR和Western blot分别检测Prohibitin 2 mRNA及其蛋白表达。用氯喹预处理HUVECs 1 h后，再用不同浓度二氢杨梅素处理12 h或10 μmol/L羰基氰化物间氯苯腙(线粒体自噬激动剂)处理6 h，流式细胞术检测线粒体自噬。另用control siRNA或Prohibitin 2 siRNA转染HUVECs后，再用二氢杨梅素处理12 h，流式细胞术检测细胞线粒体自噬。

结果

与无二氢杨梅素(0 μmol/L)处理相比，≥0.1 μmol/L二氢杨梅素处理显著降低血管内皮细胞MTDR荧光强度；同时，二氢杨梅素处理可明显增加Prohibitin 2 mRNA和蛋白表达水平(

＜0.05)；当以氯喹预处理抑制溶酶体功能后，与无二氢杨梅素处理相比，≥10 μmol/L二氢杨梅素处理可以显著增加血管内皮细胞线粒体自噬，与羰基氰化物间氯苯腙效应相一致(

＜0.05)。与control siRNA处理组相比，以siRNA转染沉默Prohibitin 2后二氢杨梅素对线粒体自噬的诱导作用被明显抑制(

＜0.05)。

结论

二氢杨梅素可通过调控Prohibitin 2增强血管内皮细胞线粒体自噬。

Abstract

Objective

To observe the effects of dihydromyricetin on mitophagy in vascular endothelial cells

and to clarify the underlying role of Prohibitin 2 in the process.

Methods

After human umbilical vein endothelial cells (HUVECs) were treated with different concentrations of dihydromyricetin (0

0.1

100 μmol/L) for 12 h

intensity of MitoTracker

Deep Red (MTDR

a widely used mitochondria-selective probe) was determined for mitochondrial staining by fluorescence microscopy and flow cytometry

and the expression of Prohibitin 2 at mRNA and protein levels as determined by qRT-PCR

and Western blotting

respectively. Furthermore

after treatment with of without control/Prohibitin 2 siRNA

HUVECs were incubated with dihydromyricetin for 12 h or carbonyl cyanide 3-chlorophenyl hydrazone (CCCP) for 6 h in the presence of the lysosomal blocker chloroquine

and then subjected to flow cytometry analysis to determine mitophagy.

Results

As compared with the non-dihydromyricetin group

dihydromyricetin (≥ 0.1μmol/L) treatment markedly decreased MTDR fluorescence levels (

0.05)

and meanwhile

the treatment significantly increased the mRNA and protein levels of Prohibitin 2 (

0.05). After pretreated with chloroquine

dihydromyricetin (≥10 μmol/L) significantly enhanced mitophagy in HUVECs

which was consistent with the effect of CCCP (

0.05). After Prohibitin 2 siRNA transfection

mitophagy induced by dihydromyricetin was significantly suppressed when compared with the control siRNA treatment group (

0.05).

Conclusion

Dihydromyricetin enhances mitophagy in vascular endothelial cells by regulating Prohibitin 2.

关键词

Keywords

references

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