Effect of pinoresinol diglucoside on bone quality in growing rats

Gaoqian XIE; Yuhai GAO; Xin BAI; Yueying HE; Keming CHEN

doi:10.16016/j.2097-0927.202107081

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Effect of pinoresinol diglucoside on bone quality in growing rats

Basic Medicine | 更新时间：2026-01-09

- Effect of pinoresinol diglucoside on bone quality in growing rats
- Journal of Army Medical University Vol. 44, Issue 2, Pages: 132-137(2022)
- 作者机构：
  
  1.730070 兰州，甘肃农业大学生命科学技术学院
  2.730050 兰州，中国人民解放军联勤保障部队第九四○医院基础医学实验室
- 作者简介：
- 基金信息：
  
  by the General Program of National Natural Science Foundation of China(81770879);the Military Logistic Scientific Research Plan(CLB18J028);the Youth Cultivation Project of No. 940 Hospital of Joint Logistics Support Force(2021yxky053)
- DOI：10.16016/j.2097-0927.202107081
  CLC： R285.5;R322.71;R681.01
- Received：12 July 2021，
  
  Revised：2021-10-09，
  
  Published：30 January 2022
- 稿件说明：
移动端阅览
Gaoqian XIE, Yuhai GAO, Xin BAI, et al. Effect of pinoresinol diglucoside on bone quality in growing rats[J]. Journal of Army Medical University, 2022, 44(2): 132-137.
DOI：

Gaoqian XIE, Yuhai GAO, Xin BAI, et al. Effect of pinoresinol diglucoside on bone quality in growing rats[J]. Journal of Army Medical University, 2022, 44(2): 132-137. DOI： 10.16016/j.2097-0927.202107081.

摘要

目的

研究松脂醇二葡萄糖苷(pinoresinol diglucoside

PDG)对生长期大鼠骨质量的影响

评价其应用于抗骨质疏松药物的潜力。

方法

将30只1月龄雌性SD大鼠按随机数字表法分为3组: 对照组(Control)、25 mg/(kg·d)的PDG处理组(PDG-25)和50 mg/(kg·d)的PDG处理组(PDG-50)

体质量(107±7)g

每组10只。Control组每天灌服等体积蒸馏水

PDG-25组和PDG-50组分别每天灌服25 mg/kg和50 mg/kg的PDG。第2、4、6周后检测大鼠全身骨密度。之后处死所有大鼠

计算重要器官的器官系数

进行micro CT扫描、骨生物力学实验以及血清骨代谢生化指标检测等。

结果

各组大鼠体质量和主要器官系数差异无统计学意义; 6周时PDG-25组和PDG-50组的全身骨密度均显著高于Control组(

0.05);PDG-50组的相对骨体积、骨小梁数和体积骨密度显著高于Control组(

0.05)

骨小梁分离度显著低于Control组(

0.05)

PDG-25组和PDG-50组的骨微结构均明显优于Control组; PDG-50组的股骨弹性模量和椎骨最大载荷显著高于Control组(

0.05);PDG-50组血清骨钙素含量显著高于Control组(

0.01)

但血清抗酒石酸酸性磷酸酶5b含量显著低于Control组(

0.01)

PDG-25组血清骨钙素含量显著高于Control组(

0.05)。

结论

PDG能有效提高生长期大鼠的骨密度、优化骨微结构、增强骨强度、促进骨形成的同时抑制骨吸收

提高骨质量。

Abstract

Objective

To determine the effect of pinoresinol diglucoside (PDG) on bone quality in growing rats and evaluate its potential as an anti-osteoporosis drug.

Methods

A total of 30 1-month-old female SD rats (body weight 107±7 g) were randomly divided into 3 groups: control group (Control)

25 mg/(kg·d) PDG treatment group (PDG-25) and 50 mg/(kg·d) PDG treatment group (PDG-50)

with 10 rats in each group. The rats in the PDG-25 group and PDG-50 group were given corresponding doses of PDG intragastrically every day

and those of the control group was given equal volume distilled water. Their body mineral density (BMD) of the whole body was measured in 2

4 and 6 weeks after treatment. In the end of 6 weeks

all rats were sacrificed

and organ coefficients of important organs were calculated. Micro-CT scanning

bone biomechanical test and serum biochemical tests of bone metabolism were performed.

Results

There were no significant differences in body weight and main organ coefficients among the groups. At 6 weeks

the BMD value was significantly higher in the PDG-25 group and PDG-50 group than the Control group (

0.05). The PDG-50 group had significantly higher relative bone volume

trabecular number and volumetric bone mineral density (

0.05)

lower trabecular separation (

0.05) than the Control group

and the bone microstructure of PDG-25 group and PDG-50 group was significantly better than that of Control group. The PDG-50 group had elevated elastic modulus of femur (

0.05)

larger maximum load of vertebrae (

0.0

5). The PDG-50 group had increased content of serum osteocalcin (

0.01)

but obviously lower content of serum tartrate resistant acid phosphatase 5b (

0.01)

when compared with the Control group. The content of serum osteocalcin in the PDG-25 group was significantly higher than that in the Control group (

0.05).

Conclusion

PDG can effectively improve bone mineral density

optimize bone microstructure

enhance bone strength

promote bone formation

inhibit bone resorption and improve bone quality.

关键词

Keywords

references

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