Auditory brainstem responses during development of MeCP2 autistic mice

Sumei YAN; Shaowen QIAN; Xianli HU; Ruiqi PANG; Zhiyue SHI; Yi ZHOU

doi:10.16016/j.2097-0927.202107039

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Auditory brainstem responses during development of MeCP2 autistic mice

Neurosciences | 更新时间：2026-01-09

- Auditory brainstem responses during development of MeCP2 autistic mice
- Journal of Army Medical University Vol. 44, Issue 2, Pages: 103-109(2022)
- 作者机构：
  
  1.400038 重庆，陆军军医大学(第三军医大学)基础医学院神经生物学教研室
  2.530004 南宁，广西大学脑与智能研究中心
  3.857000 西藏日喀则，陆军第953医院外科
- 作者简介：
- 基金信息：
  
  by the General Program of National Natural Science Foundation of China(31970932);by the General Program of National Natural Science Foundation of China(31771152)
- DOI：10.16016/j.2097-0927.202107039
  CLC： R338.8;R339.16;R749.94
- Received：07 July 2021，
  
  Revised：2021-08-23，
  
  Published：30 January 2022
- 稿件说明：
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Sumei YAN, Shaowen QIAN, Xianli HU, et al. Auditory brainstem responses during development of MeCP2 autistic mice[J]. Journal of Army Medical University, 2022, 44(2): 103-109.
DOI：

Sumei YAN, Shaowen QIAN, Xianli HU, et al. Auditory brainstem responses during development of MeCP2 autistic mice[J]. Journal of Army Medical University, 2022, 44(2): 103-109. DOI： 10.16016/j.2097-0927.202107039.

摘要

目的

探索甲基-CpG结合蛋白2(methyl CpG binding protein 2

MeCP2)过表达小鼠(MeCP2-Tg1，自闭模型小鼠)发育过程中的听力变化。

方法

在8周和14周对MeCP2-Tg1小鼠(Tg1组)和WT小鼠(WT组)进行三箱社交行为测试，检测MeCP2-Tg1小鼠的社交功能。在社交行为异常发生前后的5个发育节点(4、8、12、16、20周)记录click及纯音刺激诱发的听觉脑干电位，评估不同发育阶段Tg1小鼠的听力敏感性(阈值)、神经传导速度(潜伏期)和反应强度(波幅)。

结果

①与WT组相比，Tg1组小鼠在4周和12周对24 kHz纯音刺激表现出更高的敏感性(

0.05)，20周时对8 kHz纯音刺激表现出更低的阈值(

0.05)，但阈值降低现象在发育进程中并不持续。②Tg1组小鼠在整个发育过程中的神经传导速度正常。③与WT组相比，Tg1组小鼠表征上橄榄核的Ⅲ波幅值在8周(含)之前正常，但12周(含)之后表现出显著的持续性升高(

0.05)；表征听觉下丘的Ⅴ波幅值在8周(含)之前显著偏低(

0.05)，但12周及之后则恢复正常。

结论

MeCP2-Tg1小鼠听觉脑干反应在发育过程中出现异常，主要表现为在不同发育时期对声刺激反应幅度的增高/降低，且听力异常的发生早于社交行为异常发生。

Abstract

Objective

To explore the hearing changes in an autism model mice [MeCP2-Tg1 mice

with overexpression of methyl-CpG binding protein 2 (MeCP2)] during development.

Methods

Three-chamber social test was used to assess the social ability of MeCP2-Tg1 mice and wild-type mice at 8 and 14 weeks after born. Auditory brainstem responses evoked by click and pure tone stimulus were recorded at 5 development nodes (4

16 and 20 weeks) before and after the onset of abnormal social behaviors to assess hearing sensitivity (threshold)

neural conduction velocity (latency) and response level (amplitude) in MeCP2-Tg1 mice at different developmental stages.

Results

①Tg1 mice showed higher sensitivity to 24 kHz stimulus at 4 and 12 weeks (

0.05)

and lower hearing threshold to 8 kHz stimulus at 20 weeks as compared with wild-type mice (

0.05)

but the threshold reduction did not persist over the course of development. ②Tg1 mice had normal neural conduction velocity throughout development. ③ Compared with the wild-type mice

the amplitude of wave Ⅲ

which characterizes the superior olivary complex

was normal up to 8 weeks (inclusive) but showed a significant an

d persistent increase after 12 weeks (inclusive) (

0.05); the amplitude of wave V

which characterizes the auditory inferior colliculus

was significantly lower up to 8 weeks (inclusive) (

0.05) but showed normal after 12 weeks (inclusive).

Conclusion

The auditory brainstem responses of MeCP2-Tg1 mice are abnormal during development

mainly in the form of increased/decreased amplitude of responses to acoustic stimuli at different developmental periods

and the onset of hearing abnormalities precedes the onset of social behavior abnormalities.

关键词

Keywords

references

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