Recent advances in single-cell epigenetic sequencing technology

Bing NI; Weiwu GAO

doi:10.16016/j.1000-5404.202109020

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Recent advances in single-cell epigenetic sequencing technology

Basic Medicine | 更新时间：2026-01-09

- Recent advances in single-cell epigenetic sequencing technology
- Journal of Army Medical University Vol. 44, Issue 1, Pages: 74-78(2022)
- 作者机构：
  
  1.400038 重庆，陆军军医大学（第三军医大学）高原军事医学系病理生理学教研室
  2.2400038 重庆，陆军军医大学（第三军医大学）基础医学院全军免疫学研究所
- 作者简介：
- 基金信息：
  
  the Young Talent Promotion Project of China Association for Science and Technology(2019QNRC001);the Special Project for Scientific and Technological Innovation Ability Promotion of Army Medical University(2019XYY05);the Special Project for Scientific and Technological Innovation Ability Promotion of Army Medical University(2020XQN02)
- DOI：10.16016/j.1000-5404.202109020
  CLC： R394-33;R394.2
- Received：03 September 2021，
  
  Revised：2021-09-26，
  
  Published：15 January 2022
- 稿件说明：
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Bing NI, Weiwu GAO. Recent advances in single-cell epigenetic sequencing technology[J]. Journal of Army Medical University, 2022, 44(1): 74-78.
DOI：

Bing NI, Weiwu GAO. Recent advances in single-cell epigenetic sequencing technology[J]. Journal of Army Medical University, 2022, 44(1): 74-78. DOI： 10.16016/j.1000-5404.202109020.

摘要

单细胞测序技术是目前解码组织、器官、细胞、分子异质性的重要热门工具，其主要基于二代测序技术，根据其检测对象可分为单细胞基因组学测序技术、单细胞表观组学测序技术、单细胞转录组学测序技术，以及可同时解析多个组学的单细胞多组学测序技术。表观遗传学是连接基因组学和细胞表型的重要调控环节，其可通过染色质的可及性、DNA和组蛋白的修饰、核小体的排列、染色质的三维构象等发挥转录调控等作用。目前，已开发了多种检测不同表观层面的单细胞测序新技术，绘制了多种组织、器官、细胞的多个表观层面异质性的图谱，初步阐述了细胞转录异质性的表观决定机制。未来的新技术和新算法，以及单细胞测序技术在生命科学和医学疾病中的应用，将有望深度解码组织、器官、细胞、分子的异质性以及异质性的调控机制。

Abstract

Single-cell sequencing technologies are important hot tools for decoding the heterogeneity of tissues

organs

cells and molecules

mainly based on the next-generation sequencing technology. It mainly includes single-cell genome sequencing

single-cell epigenome sequencing and single-cell transcriptome sequencing

along with the multi-omics single-cell sequencing technology. Epigenetics is an important regulatory link between genomics and cell phenotype. It plays a role in transcriptional regulation through chromatin accessibility

DNA and histone modification

nucleosome arrangement and three-dimensional conformation of chromatin. At present

researchers have developed a variety of single-cell sequencing techniques at epigenetic level

plotted multiple heterogeneity atlases at epigenetic level for several tissues

organs and cells

and have also primarily illuminated the determinant mechanisms for the heterogeneity of cell transcription at epigenetic level. In the future

with the applications of new single-cell sequencing technologies and new algorithms in life science and medical sicences

it is expected that the heterogeneity and the underlying molecular mechanisms of tissues

organs

cells and molecules will be decoded deeply.

关键词

Keywords

references

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