Effect of novel turtle shell decoction on cycle, apoptosis, <italic style="font-style: italic">CTGF</italic>/<italic style="font-style: italic">Itgα</italic> 5 pathway and expression of <italic style="font-style: italic">Col</italic>1<italic style="font-style: italic">a</italic>1 in rat hepatic stellate cells

Xiuhua CAI; Anying DONG; Ronghua ZHANG; Ganping BAI; Ping WAN; Yi JIANG; Qi ZHU

doi:10.16016/j.1000-5404.202106056

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Effect of novel turtle shell decoction on cycle, apoptosis, CTGF/Itgα 5 pathway and expression of Col1a1 in rat hepatic stellate cells

Basic Medicine | 更新时间：2026-02-13

- Effect of novel turtle shell decoction on cycle, apoptosis, CTGF/Itgα 5 pathway and expression of Col1a1 in rat hepatic stellate cells
- Journal of Third Military Medical University Vol. 43, Issue 24, Pages: 2664-2670(2021)
- 作者机构：
  
  1.400038 重庆，陆军军医大学(第三军医大学)第一附属医院中医与风湿免疫科
  2.841000 新疆维吾尔自治区库尔勒，解放军951医院心肾呼吸科
- 作者简介：
- 基金信息：
  
  the Basic Research Military-Civil Integration Project(SWH2017JCZD-5)
- DOI：10.16016/j.1000-5404.202106056
  CLC： R285.5;R289.5;R575.5
- Received：07 June 2021，
  
  Revised：2021-07-04，
  
  Published：30 December 2021
- 稿件说明：
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Xiuhua CAI, Anying DONG, Ronghua ZHANG, et al. Effect of novel turtle shell decoction on cycle, apoptosis, CTGF/Itgα 5 pathway and expression of Col1a1 in rat hepatic stellate cells[J]. Journal of Third Military Medical University, 2021, 43(24): 2664-2670.
DOI：

Xiuhua CAI, Anying DONG, Ronghua ZHANG, et al. Effect of novel turtle shell decoction on cycle, apoptosis, CTGF/Itgα 5 pathway and expression of Col1a1 in rat hepatic stellate cells[J]. Journal of Third Military Medical University, 2021, 43(24): 2664-2670. DOI： 10.16016/j.1000-5404.202106056.

摘要

目的

探讨鳖甲煎改良方(novel turtle shell decoction，NTSD)对肝星状细胞T6(hepatic stellate cell T6，HSCT6)的周期、凋亡、结缔组织生长因子(

connective tissue growth factor

，

CTGF

)/整合素

integrin alpha

5，

Itgα

5)通路及1型胶原(

Collagen type

1，

Col

1)表达的影响。

方法

以NTSD(含生药量为0.71 g/mL)处理的HSCT6为实验(NTSD)组，鳖甲煎丸(turtle shell pills，TSP，与NTSD相同剂量)处理的HSCT6作为阳性对照(TSP)组，未处理的HSCT6为正常对照组。采用流式仪(FCM)检测各组HSCT6细胞的周期、凋亡变化。定量PCR、Western blot及免疫荧光细胞化学技术分别检测各组HSCT6细胞的

CTGF

、

Itgα

5及

Col

1的mRNA及其蛋白的表达。

结果

FCM检测结果显示，NTSD可将HSCT6阻滞于S细胞周期，并诱导其凋亡。定量PCR、Western blotting及免疫荧光细胞化学技术检测结果显示，NTSD可在mRNA与蛋白水平显著下调HSCT6的

CTGF

、

Itgα

5及

Col

1的表达，差异均有统计学意义(

0.01)。

结论

NTSD可通过下调

CTGF

Itgα

5将HSCT6阻滞于S细胞周期，诱导其凋亡，并影响其

Col

1的表达，以抑制其生长。

Abstract

Objective

To investigate the effects of novel turtle shell decoction (NTSD) on the cycle

apoptosis

connective tissue growth factor

integrin alpha

5 (

CTGF

Itgα

5) pathway and the expression of

Collagen type I

(

Col

1) in rat hepatic stellate cell line T6 (HSCT6).

Methods

HSCT6 cells was treated with NTSD containing crude drug 0.71 g/mL as experimental group

treated with turtle shell pills (TSP) at the same

dose as positive control

and cultured routinely as normal control. Cell cycle and apoptosis of HSCT6 cells were tested by flow cytometry (FCM). Real time-qPCR

Western blotting and immunofluorescence assay were employed to detect the expression changes of

CTGF

Itgα

5 and

Col

1 genes and their coding proteins in each group of HSCT6

respectively.

Results

FCM showed that NTSD treatment blocked the cells at S stage

and induced cell apoptosis. Real time-qPCR

Western blotting and immunofluorescence assay revealed that NTSD can significantly down-regulate the expression of

CTGF

Itgα

5 and

Col

1 in mRNA and protein levels in HSC-T6 cells (

0.01).

Conclusion

NTSD treatment results in cell arrest at S phage through down-regulating

CTGF

Itgα

induction of cell apoptosis

decrease of

Col

1 expression

and then inhibition of cell growth in HSCT6 cells.

关键词

Keywords

references

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⁰

Effect of novel turtle shell decoction on cycle, apoptosis, CTGF/Itgα 5 pathway and expression of Col1a1 in rat hepatic stellate cells

DOI：10.16016/j.1000-5404.202106056

摘要

Abstract

关键词

Keywords

references