Effect of genistein on proliferation and differentiation of osteoblasts isolated and cultured from ovariectomized rats

Yanfang XIE; Lijuan YOU; Julin ZHANG; Qinni HU; Tao ZHANG; Juan LI; Yuhai GAO

doi:10.16016/j.1000-5404.202106046

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Effect of genistein on proliferation and differentiation of osteoblasts isolated and cultured from ovariectomized rats

Basic Medicine | 更新时间：2026-01-09

- Effect of genistein on proliferation and differentiation of osteoblasts isolated and cultured from ovariectomized rats
- Journal of Third Military Medical University Vol. 43, Issue 24, Pages: 2671-2676(2021)
- 作者机构：
  
  1.730050 兰州，中国人民解放军联勤保障部队第940医院检验科
  2.730050 兰州，甘肃省妇幼保健院健康管理中心
  3.730050 兰州，中国人民解放军联勤保障部队第940医院基础医学实验室
- 作者简介：
- 基金信息：
  
  the Civilian Project of No. 940 Hospital of Joint Logistics Support Force of Chinese PLA(2021yxky050);the Project of Youth Science and Technology Foundation of Gansu Province(20JR5RA589)
- DOI：10.16016/j.1000-5404.202106046
  CLC： R282.71;R322.71;R329.28
- Received：06 June 2021，
  
  Revised：2021-08-30，
  
  Published：30 December 2021
- 稿件说明：
移动端阅览
Yanfang XIE, Lijuan YOU, Julin ZHANG, et al. Effect of genistein on proliferation and differentiation of osteoblasts isolated and cultured from ovariectomized rats[J]. Journal of Third Military Medical University, 2021, 43(24): 2671-2676.
DOI：

Yanfang XIE, Lijuan YOU, Julin ZHANG, et al. Effect of genistein on proliferation and differentiation of osteoblasts isolated and cultured from ovariectomized rats[J]. Journal of Third Military Medical University, 2021, 43(24): 2671-2676. DOI： 10.16016/j.1000-5404.202106046.

摘要

目的

研究金雀异黄酮(Genistein，GEN)对去势大鼠分离培养的成骨细胞增殖及分化的影响。

方法

选取3月龄SD雌性大鼠30只，分为假手术组(Sham)、切除卵巢模型组(OVX)和GEN治疗组(OVX+GEN)。双能X射线骨密度仪检测大鼠全身骨密度。各组选取3只最接近其骨密度均值的大鼠麻醉后处死，无菌分离培养成骨细胞。CCK-8检测细胞增殖能力，化学法检测碱性磷酸酶(ALP)活性，RT-PCR检测各组成骨细胞中骨形成相关基因

Runx

-2、

BMP

-2的表达情况；钙化结节染色观察钙化结节阳性克隆数并计算钙化面积。

结果

全身骨密度检测结果显示，OVX组全身骨密度显著低于Sham组，而OVX+GEN组全身骨密度显著高于OVX组，差异均有统计学意义(

0.01)。细胞增殖、ALP活性检测、RT-qPCR检测成骨性基因的表达以及钙化结节面积结果均表明，OVX组与Sham组相比均显著降低(

0.01)，而OVX+GEN组与OVX组相比均显著增加(

0.01)。

结论

GEN提高去卵巢大鼠骨密度可能与其促进成骨细胞增殖和成熟矿化能力有关。

Abstract

Objective

To determine the effects of genistein (GEN) on the proliferation and differentiation of osteoblasts isolated and cultured from ovariectomized rats.

Methods

Thirty 3-month-old female SD rats were divided into sham operation group (Sham)

ovariectomy model group (OVX) and Gen treatment group (OVX+GEN). Their bone mineral density of whole body of was measured by dual energy X-ray absorptiometry. Three rats out of each group with bone mineral density closest to the mean value were killed after anesthesia

and osteoblasts were isolated and cultured aseptically. Cell proliferation was detected by CCK-8 assay

alkaline phosphatase (ALP) activity was detected by chemical method

and the expression of bone formation related genes

Runx

-2 and

BMP

-2 in the obtained osteoblasts was detected by RT-PCR. The number of positive clones of calcified nodules was observed after

staining and the calcified area was calculated.

Results

The results of whole-body bone mineral density showed that the whole-body bone mineral density was significantly lower in the OVX group than the sham group

while that in the OVX+GEN group was significantly higher than that in the OVX group (

0.01). The OVX group had significantly lower cell proliferation

ALP activity

expression levels of Runx-2 and BMP-2 and calcified nodule area when compared with the sham group (

0.01)

while the OVX +GEN group had obviously higher results of above tests than the OVX group (

0.01).

Conclusion

Gen can improve the bone mineral density of osteoporosis in OVX rats

which may be related to its ability to promote osteoblast proliferation

maturation and mineralization.

关键词

Keywords

references

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