Effect of long non-coding RNA DUXAP8 on proliferation, cycle and apoptosis of ovarian cancer cells by regulating miR-4677-3p

Cen CHEN; Dan SU; Ningwei LI; Qin JIANG; Yazhou WANG

doi:10.16016/j.1000-5404.202106001

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Effect of long non-coding RNA DUXAP8 on proliferation, cycle and apoptosis of ovarian cancer cells by regulating miR-4677-3p

Basic Medicine | 更新时间：2026-01-09

- Effect of long non-coding RNA DUXAP8 on proliferation, cycle and apoptosis of ovarian cancer cells by regulating miR-4677-3p
- Journal of Third Military Medical University Vol. 43, Issue 24, Pages: 2656-2663(2021)
- 作者机构：
  
  1.610072 成都，四川省人民医院妇产科
  2.611130 成都，成都市第五人民医院外科
- 作者简介：
- 基金信息：
  
  the Technology Innovation Research and Development Project of Chengdu Science and Technology Bureau(2019-YF05-00244-SN)
- DOI：10.16016/j.1000-5404.202106001
  CLC： R394.3;R730.23;R737.31
- Received：01 June 2021，
  
  Revised：2021-09-26，
  
  Published：30 December 2021
- 稿件说明：
移动端阅览
Cen CHEN, Dan SU, Ningwei LI, et al. Effect of long non-coding RNA DUXAP8 on proliferation, cycle and apoptosis of ovarian cancer cells by regulating miR-4677-3p[J]. Journal of Third Military Medical University, 2021, 43(24): 2656-2663.
DOI：

Cen CHEN, Dan SU, Ningwei LI, et al. Effect of long non-coding RNA DUXAP8 on proliferation, cycle and apoptosis of ovarian cancer cells by regulating miR-4677-3p[J]. Journal of Third Military Medical University, 2021, 43(24): 2656-2663. DOI： 10.16016/j.1000-5404.202106001.

摘要

目的

探讨长链非编码RNA DUXAP8通过调控miR-4677-3p对卵巢癌细胞的增殖、周期和凋亡的影响。

方法

通过RT-qPCR检测卵巢癌组织和细胞中的DUXAP8和miR-4677-3p的表达。然后将SKOV3细胞分为：si-NC组、si-DUXAP8组、miR-NC组、miR-4677-3p组、si-DUXAP8+anti-miR-NC组和si-DUXAP8+anti-miR-4677-3p组；CCK8、克隆形成实验检测各组细胞活力；流式细胞术检测各组细胞周期和凋亡情况；Western blot检测Cyclin D1、Bcl-2和Bax蛋白表达；双荧光素酶报告实验检测DUXAP8和miR-4677-3p的关系。

结果

与癌旁组织和正常卵巢上皮细胞比较，DUXAP8在卵巢癌组织(0.98±0.25)

(4.14±0.72)中表达明显增加，SKOV3、OV90、CaOV3表达明显增加(1.03±0.08

3.26±0.33、2.75±0.29、2.54±0.26)，差异具有统计学意义(

0.05)。干扰DUXAP8或过表达miR-4677-3p可以抑制卵巢癌细胞的增殖、降低凋亡率、阻滞细胞周期，差异具有统计学意义(

0.05)。DUXAP8调控miR-4677-3p的表达，抑制miR-4677-3p可以部分回复干扰DUXAP8对卵巢癌细胞增殖、周期和凋亡的作用，差异具有统计学意义(

0.05)。

结论

干扰DUXAP8能够抑制卵巢癌细胞增殖、诱导细胞凋亡、阻滞细胞周期，其作用机制可能与上调miR-4677-3p有关。

Abstract

Objective

To investigate the effects of long non-coding RNA DUXAP8 on the proliferation

cell cycle and apoptosis of ovarian cancer cells by regulating miR-4677-3p.

Methods

The expression of DUXAP8 and miR-4677-3p in ovarian cancer tissues and cell lines (SKOV3

OV90 and CaOV3) was detected by RT-qPCR. Then SKOV3 cells were divided into 6 groups

namely

si-NC group

si-DUXAP8 group

miR-NC group

miR-4677-3p group

si-DUXAP8+anti-miR-NC group and si-DUXAP8+anti-miR-4677-3p group. CCK-8 assay and clone formation assay were adopted to detect cell viability

flow cytometry to observe cell apoptosis and cycle

Western blotting to measure the expression levels of Cyclin D1

Bcl-2 and Bax

and dual luciferase reporter assay system to analyze the relationship of DUXAP8 and miR-4677-3p.

Results

When compared with adjacent tissues and normal ovarian epithelial cells

the expression level of DUXAP8 was significantly lower in ovarian cancer tissues (0.98±0.25

4.14±0.72) and that of SKOV3

OV90

CaOV3 was obviously higher (1.03±0.08

3.26±0.33、2.75±0.29、2.54±0.26

all

0.05). Interference with DUXAP8 or overexpression of miR-4677-3p inhibited the proliferation

reduced the rate of apoptosis

and arrested the cell cycle of ovarian cancer cells (

0.05). DUXAP8 regulated the expression of miR-4677-3p

and inhibiting miR-4677-3p partially restore and interfere with the effects of DUXAP8 on the proliferation

cycle and apoptosis of ovarian cancer cells (

0.05).

Conclusion

Interference with DUXAP8 can inhibit the proliferation of ovarian cancer cells

induce cell apoptosis

and block cell cycle

the mechanism may be related to the up-regulation of miR-4677-3p.

关键词

Keywords

references

LHEUREUX S, GOURLEY C, VERGOTE I, et al . Epithelial ovarian cancer[J ] . Lancet, 2019, 393(10177): 1240-1253. DOI: 10.1016/s0140-6736(18)32552-2.

HEITZ F, HARTER P, ÅVALL-LUNDQVIST E, et al . Early tumor regrowth is a contributor to impaired survival in patients with completely resected advanced ovarian cancer. An exploratory analysis of the Intergroup trial AGO-OVAR 12[J ] . Gynecol Oncol, 2019, 152(2): 235-242. DOI: 10.1016/j.ygyno.2018.11.008.

李小琳, 韩艳艳, 杨雪, 等. 卵巢癌的分子靶向治疗进展[J]. 实用癌症杂志, 2020, 35(4): 695-696. DOI: 10.3969/j.issn.1001-5930.2020.04.048.

LI X L, HAN Y Y, YANG X, et al . Progress in molecular targeted therapy of ovarian cancer[J ] . Pract J Cancer, 2020, 35(4): 695-696. DOI: 10.3969/j.issn.1001-5930.2020.04.048.

WANG J Y, LU A Q, CHEN L J. LncRNAs in ovarian cancer[J]. Clin Chim Acta, 2019, 490: 17-27. DOI: 10.1016/j.cca.2018.12.013.

MENG Q, LI Z, PAN J, et al . Long noncoding RNA DUXAP8 regulates proliferation and apoptosis of ovarian cancer cells via targeting miR-590-5p[J ] . Hum Cell, 2020, 33(4): 1240-1251. DOI: 10.1007/s13577-020-00398-8.

苏英杰, 高晨曦, 张颐. MicroRNA在卵巢癌诊疗中的作用[J]. 肿瘤学杂志, 2021, 26(1): 4-8. DOI: 10.11735/j.issn.1671-170X.2021.01.B002.

SU Y J, GAO C X, ZHANG Y. Research progress on microRNA in diagnosis and treatment of ovarian cancer[J]. J Chin Oncol, 2021, 26(1): 4-8. DOI: 10.11735/j.issn.1671-170X.2021.01.B002.

RESNICK K E, ALDER H, HAGAN J P, et al . The detection of differentially expressed microRNAs from the serum of ovarian cancer patients using a novel real-time PCR platform[J ] . Gynecol Oncol, 2009, 112(1): 55-59. DOI: 10.1016/j.ygyno.2008.08.036.

HAN B. LncRNA LINC02418 regulates proliferation and apoptosis of non-small cell lung cancer cells by regulating miR-4677-3p/SEC61G[J]. Eur Rev Med Pharmacol Sci, 2019, 23(23): 10354-10362. DOI: 10.26355/eurrev_201912_19673.

ZHENG M, HU Y, GOU R, et al . Identification three LncRNA prognostic signature of ovarian cancer based on genome-wide copy number variation[J ] . Biomed Pharmacother, 2020, 124: 109810. DOI: 10.1016/j.biopha.2019.109810.

杜晓琴. LncRNA KCNQ1OT1在卵巢癌组织中的表达及其临床意义[J]. 肿瘤防治研究, 2017, 44(5): 329-333. DOI: 10.3971/j.issn.1000-8578.2017.05.004.

DU X Q. Expression and clinical significance of LncRNA KCNQ1OT1 in ovarian cancer tissues[J]. Cancer Res Prev Treat, 2017, 44(5): 329-333. DOI: 10.3971/j.issn.1000-8578.2017.05.004.

CHEN M H, FAN M, YANG J L, et al . Identification of potential oncogeniclong non-coding RNA set as a biomarker associated with colon cancer prognosis[J ] . J Environ Pathol Toxicol Oncol, 2020, 39(1): 39-49. DOI: 10.1615/JEnvironPatholToxicolOncol.2020032351.

CHEN M, ZHENG Y, XIE J, et al . Integrative profiling analysis identifies the oncogenic long noncoding RNA DUXAP8 in oralcancer[J ] . Anticancer Drugs, 2020, 31(8): 792-798. DOI: 10.1097/cad.0000000000000936.

JIANG B, HAILONG S, YUAN J, et al . Identification of oncogenic long noncoding RNA SNHG12 and DUXAP8 in human bladder cancer through a comprehensive profiling analysis[J ] . Biomed Pharmacother, 2018, 108: 500-507. DOI: 10.1016/j.biopha.2018.09.025.

LIAN Y, YANG J, LIAN Y, et al . DUXAP8, a pseudogene derived lncRNA, promotes growth of pancreatic carcinoma cells by epigenetically silencing CDKN1A and KLF2[J ] . Cancer Commun (Lond), 2018, 38(1): 64. DOI: 10.1186/s40880-018-0333-9.

ZHANG H P, CHU K Q, ZHENG C X, et al . Pseudogene DUXAP8 promotes cell proliferation and migration of hepatocellular carcinoma by sponging miR-490-5p to induce BUB1 expression[J ] . Front Genet, 2020, 11: 666. DOI: 10.3389/fgene.2020.00666.

ZHAO X, HAO S, WANG M Q, et al . Knockdown of pseudogene DUXAP8 expression in glioma suppresses tumor cell proliferation[J ] . Oncol Lett, 2019, 17(3): 3511-3516. DOI: 10.3892/ol.2019.9994.

HE L Q, ZHU W, CHEN Q, et al . Ovarian cancer cell-secreted exosomal miR-205 promotes metastasis by inducing angiogenesis[J ] . Theranostics, 2019, 9(26): 8206-8220. DOI: 10.7150/thno.37455.

ZHAO W, HAN T, LI B, et al . MiR-552 promotes ovarian cancer progression by regulating PTEN pathway[J ] . J Ovarian Res, 2019, 12(1): 121. DOI: 10.1186/s13048-019-0589-y.

ZHANG Z, ZHANG L, WANG B, et al . MiR-337-3p suppresses proliferation of epithelial ovarian cancer by targeting PIK3CA andPIK3CB[J ] . Cancer Lett, 2020, 469: 54-67. DOI: 10.1016/j.canlet.2019.10.021.

XIA B, YANG S, LIU T, et al . MiR-211 suppresses epithelial ovarian cancer proliferation and cell-cycle progression by targeting Cyclin D1 and CDK6[J ] . Mol Cancer, 2015, 14: 57. DOI: 10.1186/s12943-015-0322-4.

ZHONG Y, WANG J, LV W, et al . LncRNA TTN-AS1 drives invasion and migration of lung adenocarcinoma cells via modulation of miR-4677-3p/ZEB1 axis[J ] . J Cell Biochem, 2019, 120(10): 17131-17141. DOI: 10.1002/jcb.28973.

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