Neuronal discharge activity in mouse medial prefrontal cortex during associative motor learning

Yubo HU; Libin ZHANG; Hao CHEN; Yan XIONG

doi:10.16016/j.1000-5404.202105119

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Neuronal discharge activity in mouse medial prefrontal cortex during associative motor learning

Neurosciences | 更新时间：2026-01-09

- Neuronal discharge activity in mouse medial prefrontal cortex during associative motor learning
- Journal of Third Military Medical University Vol. 43, Issue 24, Pages: 2618-2624(2021)
- 作者机构：
  
  1.400042 重庆，陆军军医大学(第三军医大学)大坪医院骨科
  2.833000 新疆维吾尔自治区乌苏，中国人民解放军第948医院普通外科
  3.400038 重庆，陆军军医大学(第三军医大学)基础医学院基础医学教学实验中心
- 作者简介：
- 基金信息：
  
  the General Program of National Natural Science Foundation of China(81871039)
- DOI：10.16016/j.1000-5404.202105119
  CLC： R338.25;R338.64;R338.8
- Received：11 May 2021，
  
  Revised：2021-10-13，
  
  Published：30 December 2021
- 稿件说明：
移动端阅览
Yubo HU, Libin ZHANG, Hao CHEN, et al. Neuronal discharge activity in mouse medial prefrontal cortex during associative motor learning[J]. Journal of Third Military Medical University, 2021, 43(24): 2618-2624.
DOI：

Yubo HU, Libin ZHANG, Hao CHEN, et al. Neuronal discharge activity in mouse medial prefrontal cortex during associative motor learning[J]. Journal of Third Military Medical University, 2021, 43(24): 2618-2624. DOI： 10.16016/j.1000-5404.202105119.

摘要

目的

研究联合型运动学习过程中小鼠内侧前额叶皮层(medial prefrontal cortex

mPFC)不同类型神经元放电活动变化的规律及其与学习行为的相关性。

方法

以痕迹性眨眼条件反射(trace eyeblink conditioning

tEBC)作为联合型运动学习的行为模型。以150 ms脉冲蓝色LED光作为条件刺激(conditioned stimulus

CS)，100 ms角膜吹气作为非条件刺激(unconditioned stimulus

US)，进行每日100次的CS-US配对训练。连续5 d，训练小鼠(

=4)建立tEBC。在此过程中，使用钨丝tetrode电极记录小鼠mPFC神经元放电活动。根据神经元放电的频率和峰电位谷-峰波宽，将mPFC神经元区分为假定的锥体细胞(

=174)和中间神经元(

=40)，并分析两类神经元活动在tEBC建立早期和后期与条件眨眼反应(conditioned eyeblink responses

CR)行为的相关性。

结果

通过5 d CS-US配对训练，小鼠CR发生率从训练第1天的(38.1±8.2)%上升到第5天的(66.7±10.0)% (

0.001)。统计学分析显示

相较于CR习得早期，CR习得后期CS所诱发的锥体细胞放电活动显著降低(

0.05)。与此相适应，CR习得后期CS诱发的中间神经元放电活动显著增高(

0.05)，表现为CS诱发增强型放电的中间神经元比例显著增高(早期：25.0%；后期：66.7%，

0.05)。此外，锥体细胞在CR出现和无CR出现时的CS诱发放电活动无显著区别(

0.05)，而中间神经元在CR出现时CS诱发放电活动则显著更高(

0.05)。

结论

在联合型运动学习过程中，小鼠mPFC锥体细胞和中间神经元的放电活动随学习会发生相反的变化，提示它们可能以不同方式参与联合型运动学习。中间神经元放电活动与CR出现更为相关。

Abstract

Objective

To investigate the discharge profiles of different types of neurons in the medial prefrontal cortex (mPFC) during associative motor learning and their correlation with learning behaviors.

Methods

Trace eyeblink conditioning (tEBC) was used as the behavioral model of associative motor learning. Freely-moving mice (

=4) were trained to acquire tEBC for 5 consecutive days. During daily tEBC training

the mice were received 100 paired presentations of the conditioned stimulus (CS

150-ms blue LED pulse) and unconditioned stimulus (US

100-ms corneal airpuff). Tungsten tetrodes were utilized to record the discharge activity of mPFC neurons. The mPFC neurons were categorized into putative pyramidal cells (

=174) and putative interneurons (

=40) according to the discharge rate and width of the mean spike waveform from trough to peak for each neuron. The correlation between the activity of distinct mPFC neurons and the performance of conditioned eyeblink responses (CR) was analyzed in either the early- or the late-learning stage.

Results

After 5 days' CS-US paired training

the mice exhibited increased CR incidence from (38.1±8.2)% on the 1st day to (66.7±10.0)% on the 5th day (

0.001). The statistical analysis revealed that the CS-evo

ked discharge activity of putative mPFC pyramidal cells at the late-leaning stage was significantly weaker than the early-learning stage (

0.05). In contrast

the CS-evoked discharge activity of putative interneurons at the late-learning stage was significantly greater than that at the early-learning stage (

0.05). Consistent with this

the proportion of CS-evoked increased firing interneurons was significantly greater at the late-learning stage (66.7%

14/21) than the early-learning stage (25.0%

4/16

0.05). Moreover

there was no significant difference in the CS-evoked activity of mPFC pyramidal cells between the CR and the non-CR states (

0.05)

whereas the CS-evoked activity of mPFC interneurons in the presence of CRs was stronger than in the lack of CRs (

0.05).

Conclusion

During the acquisition of tEBC

the change of CS-evoked firing activities in mPFC interneurons is opposite to that in mPFC pyramidal cells

suggesting that these 2 types of mPFC neurons might participate in associative motor learning in different ways. In particular

the CS-evoked firing activity of mPFC interneurons is closely correlated with the CR performance.

关键词

Keywords

references

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