Single cell sequencing in the study of hematopoietic system: application and perspects
- Journal of Third Military Medical University Vol. 43, Issue 17, Pages: 1603-1610(2021)
- 作者机构：
- 作者简介：
- 基金信息：the National Science Foundation for Distinguished Young Scholars of China(81725019);the Key Program of National Natural Science Foundation of China(81930090);and the Major Special Project of Military Logistics Scientific Research(ALJ19J002)
DOI：10.16016/j.1000-5404.202105013
CLC：Received：07 May 2021，
Published：15 September 2021
- 稿件说明：
移动端阅览
Junping WANG, Naicheng CHEN. Single cell sequencing in the study of hematopoietic system: application and perspects[J]. Journal of Third Military Medical University, 2021, 43(17): 1603-1610.
Junping WANG, Naicheng CHEN. Single cell sequencing in the study of hematopoietic system: application and perspects[J]. Journal of Third Military Medical University, 2021, 43(17): 1603-1610. DOI： 10.16016/j.1000-5404.202105013.
摘要
目的
2
单细胞测序(single cell sequencing，SCS)是一项在细胞个体层面进行精准化分析的技术。它提供了全新的角度去识别单个细胞的种类、功能、状态，以及与其他细胞的相互关联，极大深化了人们对细胞异质性、基因与分子功能多样性等方面的理解。随着各个技术环节的不断优化，SCS技术已广泛应用于多个研究领域，其中造血系统研究便是该技术运用的范例。SCS不仅在基础研究中，协助完成了生理和病理生理条件下的相关造血细胞图谱的构建，探索了血细胞发育分化的可能路径，而且在临床诊治过程中，有效推动疾病发生、进展和转归等机制的破解，促进个体疗效差异显著、易于复发等现象的潜在因素的挖掘。现就SCS的技术概况、发展趋势及其在造血系统的应用进行评述，以期为后续SCS技术的积极与合理应用提供参考。
Abstract
Single cell sequencing (SCS) is a technique for precise analysis of cells at an individual level
which provides a new perspective on the identification of individual cell types
functions
states and interactions with other cells
and greatly deepens the understanding of cellular heterogeneity and genetic and molecular functional diversity. With the continuous optimization of various technical aspects
SCS technology has been widely used in many research areas
and the study of the hematopoietic system is an example of its application. SCS has not only assisted in the construction of relevant hematopoietic cell profiles under physiological and pathophysiological conditions in basic research
but also explored possible pathways of hematopoietic cell development and differentiation in clinical practice. Moreover
in the process of clinical diagnosis and treatment
it can effectively promote the deciphering of mechanisms of disease occurrence
progression and prognosis
and facilitate the exploration of potential factors of phenomena such as significant differences in individual outcomes and ease of relapse. Therefore
this paper reviews the technical overview and development trend of SCS and its application in the hematopoietic system
with a view to providing references for subsequent positive and reasonable application of SCS.
关键词
Keywords
references
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