Cigarette smoke extract activates NLRP3 inflammasome to promote invasion of lung adenocarcinoma PC-9 cells

Jiukang LI; Hong CHEN

doi:10.16016/j.1000-5404.202104196

您当前的位置：

首页 >

文章列表页 >

Cigarette smoke extract activates NLRP3 inflammasome to promote invasion of lung adenocarcinoma PC-9 cells

Basic Medicine | 更新时间：2026-01-09

- Cigarette smoke extract activates NLRP3 inflammasome to promote invasion of lung adenocarcinoma PC-9 cells
- Journal of Third Military Medical University Vol. 43, Issue 20, Pages: 2214-2219(2021)
- 作者机构：
  
  400016 重庆，重庆医科大学附属第一医院呼吸与危重症医学科
- 作者简介：
- 基金信息：
- DOI：10.16016/j.1000-5404.202104196
  CLC： R363.13;R730.23;R734.2
- Received：29 April 2021，
  
  Revised：2021-07-05，
  
  Published：30 October 2021
- 稿件说明：
移动端阅览
Jiukang LI, Hong CHEN. Cigarette smoke extract activates NLRP3 inflammasome to promote invasion of lung adenocarcinoma PC-9 cells[J]. Journal of Third Military Medical University, 2021, 43(20): 2214-2219.
DOI：

Jiukang LI, Hong CHEN. Cigarette smoke extract activates NLRP3 inflammasome to promote invasion of lung adenocarcinoma PC-9 cells[J]. Journal of Third Military Medical University, 2021, 43(20): 2214-2219. DOI： 10.16016/j.1000-5404.202104196.

摘要

目的

探讨烟草烟雾(cigarette smoke extract

CSE)提取物对肺腺癌PC-9细胞侵袭能力的影响及机制。

方法

体外培养PC-9细胞，与0%、1%、2%、4%烟草烟雾提取物共培养96 h。Transwell实验检测细胞侵袭能力，Western blot检测NLRP3、Caspase-1、IL-1β及MMP-2蛋白表达。予以4%烟草烟雾提取物与MCC950 (NLRP3抑制剂)共同处理96 h后，细胞侵袭实验检测细胞侵袭能力，Western blot检测NLRP3、Caspase-1、IL-1β及MMP-2蛋白表达。

结果

与对照组相比，烟草提取物处理组中发生侵袭的PC-9细胞数量呈浓度(1%~4% CSE)依耐性增多(

0.05，

0.01)。NLRP3、Caspase-1、IL-1β及MMP-2的蛋白水平在烟草提取物处理组中呈浓度依耐性上调(

0.01)。MCC950干预后，可显著抑制烟草烟雾提取物诱导的PC-9侵袭细胞的增多(

0.05)以及NLRP3、Caspase-1、IL-1β、MMP-2表达的上调(

0.05)。

结论

烟草烟雾提取物可增强肺腺癌细胞侵袭能力，可能与激活NLRP3炎性小体有关。

Abstract

Objective

To investigate the effect and underlying mechanism of cigarette smoke extract (CSE) on invasion ability of lung adenocarcinoma PC-9 cells.

Methods

PC-9 cells were cultured in the medium containing 0%

2% and 4% tobacco smoke extract for 96 h

respectively. The invasive ability of the cells was detected by Transwell assay. The protein levels of NLRP3

Caspase-1

IL-1β and MMP-2 were measured with Western blotting. After co-treatment with 4% CSE and NLRP3 inhibitor

MCC950

for 96 h

Transwell assay was employed again for the invasive ability of the cells

and Western blotting for the expression of NLRP3

Caspase-1

IL-1β and MMP-2.

Results

CSE significantly increased the invasive ability of PC-9 cells (

0.05

0.01) and promoted the protein expression of NLRP3

Caspase-1

IL-1β and MMP-2(

0.01) in dose-dependent manners. The intervention of MCC950 obviously inhibited the enhanced invasive ability of PC-9 cells and the up-regulation of NLRP3

Caspase-1

IL-1β and MMP-2 induced by CSE.

Conclusion

CSE enhances the invasive ability of lung adenocarcinoma cells

which may be associated with the activation of NLRP3 inflammasome.

关键词

Keywords

references

LIAO H L, LIANG Y Q, KANG L, et al . miR-454-3p inhibits non-small cell lung cancer cell proliferation and metastasis by targeting TGFB 2 [J ] . Oncol Rep, 2021, 45(5): 67. DOI: 10.3892/or.2021.8018..

FENG R M, ZONG Y N, CAO S M, et al . Current cancer situation in China: good or bad news from the 2018 Global Cancer Statistics?[J ] . Cancer Commun, 2019, 39(1): 22. DOI: 10.1186/s40880-019-0368-6.

LIAO K, YONG C W, HUA K. SB431542 inhibitedcigarette smoke extract induced invasiveness of A549 cells via the TGF-β1/Smad2/MMP3 pathway[J ] . Oncol Lett, 2018, 15(6): 9681-9686. DOI: 10.3892/ol.2018.8556.

GOPALAKRISHNA R, CHEN Z H, GUNDIMEDA U. Tobacco smoke tumor promoters, catechol and hydroquinone, induce oxidative regulation of protein kinase C and influence invasion and metastasis of lung carcinoma cells[J]. Proc Natl Acad Sci USA, 1994, 91(25): 12233-12237. DOI: 10.1073/pnas.91.25.12233.

师萌, 王晓慧, 邓亚菲, 等. 烟草诱导的M2型巨噬细胞对肺癌细胞侵袭转移的影响[J]. 中国细胞生物学学报, 2019, 41(6): 1107-1113.

SHI M, WANG X H, DENG Y F, et al . The effects of CSE-induced M2 polarized macrophages on lung carcinoma invasion and migration[J ] . Chin J Cell Biol, 2019, 41(6): 1107-1113.

GOMEZ-LARRAURI A, OURO A, TRUEBA M, et al . Regulation of cell growth, survival and migration by ceramide 1-phosphate—implications in lung cancer progression and inflammation[J ] . Cell Signal, 2021, 83: 109980. DOI: 10.1016/j.cellsig.2021.109980.

SHAO B Z, WANG S L, PAN P, et al . Targeting NLRP3 inflammasome in inflammatory bowel disease: putting out the fire of inflammation[J ] . Inflammation, 2019, 42(4): 1147-1159. DOI: 10.1007/s10753-019-01008-y.

WANG X, SHAO Q H, ZHOU H, et al . Ginkgolide B inhibits lung cancer cells promotion via beclin-1-dependent autophagy[J ] . BMC Complement Med Ther, 2020, 20(1): 194. DOI: 10.1186/s12906-020-02980-x..

廖科, 陈虹, 赵绿翠, 等. 磷脂转运蛋白参与烟草提取物诱导的Ⅱ型肺泡上皮细胞凋亡[J]. 南方医科大学学报, 2015,35(7): 941-946. DOI: 10.3969/j.issn.1673-4254.2015.07.03.

LIAO K, CHEN H, ZHAO L C, et al . Role of phospholipid transfer protein in cigarette smoke-induced apoptosis of RLE-6TN cells[J ] . J South Med Univ, 2015, 35(7): 941-946. DOI: 10.3969/j.issn.1673-4254.2015.07.03.

LOPEZJ I, CAMENISCH T D, STEVENS M V, et al . CD44 attenuates metastatic invasion during breast cancer progression[J ] . Cancer Res, 2005, 65(15): 6755-6763. DOI: 10.1158/0008-5472.CAN-05-0863.

LI Y, LIU H, HUANGY Y, et al . Suppression of endoplasmic reticulum stress-induced invasion and migration of breast cancer cells through the downregulation of heparanase[J ] . Int J Mol Med, 2013, 31(5): 1234-1242. DOI: 10.3892/ijmm.2013.1292.

LI Y, LI Y, LIU J, et al . Expression levels of microRNA-145 and microRNA-10b are associated with metastasis in non-small cell lung cancer[J ] . Cancer Biol Ther, 2016, 17(3): 272-279. DOI: 10.1080/15384047.2016.1139242.

MOREIRAD M, ARONSON W J, TERRIS M K, et al . Cigarette smoking is associated with an increased risk of biochemical disease recurrence, metastasis, castration-resistant prostate cancer, and mortality after radical prostatectomy: results from the SEARCH database[J ] . Cancer, 2014, 120(2): 197-204. DOI: 10.1002/cncr.28423.

DI CELLO F, FLOWERSV L, LI H L, et al . Cigarette smoke induces epithelial to mesenchymal transition and increases the metastatic ability of breast cancer cells[J ] . Mol Cancer, 2013, 12: 90. DOI: 10.1186/1476-4598-12-90.

GOPALAKRISHNA R, CHENZ H, GUNDIMEDA U. Tobacco smoke tumor promoters, catechol and hydroquinone, induce oxidative regulation of protein kinase C and influence invasion and metastasis of lung carcinoma cells[J]. Proc Natl Acad Sci USA, 1994, 91(25): 12233-12237. DOI: 10.1073/pnas.91.25.12233.

SONG X L, WEI C R, LI X Q. The potential role and status of IL-17 family cytokines in breast cancer[J]. Int Immunopharmacol, 2021, 95: 107544. DOI: 10.1016/j.intimp.2021.107544.

买新佳, 黄婧娟, 陈洪雷. 新兴免疫检查点VISTA在肿瘤与炎症中的作用[J]. 武汉大学学报(医学版), 2020, 41(2): 275-279. DOI: 10.14188/j.1671-8852.2019.0311.

MAI X J, HUANG J J, CHEN H L. Function of an emerging immune checkpoint-VISTA in the cancer and inflammation[J]. Med J Wuhan Univ, 2020, 41(2): 275-279. DOI: 10.14188/j.1671-8852.2019.0311.

ZHANG M Y, JIANG Y X, YANG Y C, et al . Cigarette smoke extract induces pyroptosis in human bronchial epithelial cells through the ROS/NLRP3/caspase-1 pathway[J ] . Life Sci, 2021, 269: 119090. DOI: 10.1016/j.lfs.2021.119090.

阮萍. 炎症小体NLRP3在肿瘤中的作用[J]. 医学理论与实践, 2021, 34(1): 23-25. DOI: 10.19381/j.issn.1001-7585.2021.01.008.

RUAN P. The role of NLRP3 inflammasome in tumor[J]. J Med Theory Pract, 2021, 34(1): 23-25. DOI: 10.19381/j.issn.1001-7585.2021.01.008.

DENG R, ZHANGH L, HUANG J H, et al . MAPK1/3 kinase-dependent ULK1 degradation attenuates mitophagy and promotes breast cancer bone metastasis[J ] . Autophagy, 2020: 1-19. DOI: 10.1080/15548627.2020.1850609.

LINY S, TSAI K L, CHEN J N, et al . Mangiferin inhibits lipopolysaccharide-induced epithelial-mesenchymal transition (EMT) and enhances the expression of tumor suppressor gene PER1 in non-small cell lung cancer cells[J ] . Environ Toxicol, 2020, 35(10): 1070-1081. DOI: 10.1002/tox.22943.

WANG M, NAIM H, HUANG R Y, et al . High-throughput functional profiling of single adherent cells via hydrogel drop-screen[J ] . Lab Chip, 2021, 21(4): 764-774. DOI: 10.1039/d0lc01294g..

MCALLISTERM J, CHEMALY M, EAKIN A J, et al . NLRP3 as a potentially novel biomarker for the management of osteoarthritis[J ] . Osteoarthr Cartilage, 2018, 26(5): 612-619. DOI: 10.1016/j.joca.2018.02.901.

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Effects of SOCS3 gene on migration and invasion of lung adenocarcinoma cell line A549

Effects of LncRNA FGD5-AS1 on migration and invasion of gastric cancer cells by regulating miR-302b-3p/E2F1 axis

Expression of miR-616 in osteosarcoma and its role in proliferation, apoptosis, migration and invasion of tumor cells

Expression of β-arrestin1 in oral squamous cell carcinoma and its effect on cell proliferation, migration and invasion

Effect of calumenin on metastasis and invasion of gastric cancer and prognosis of patients

Related Author

余祖滨

白莉

姚珂

张国强

闵家新

肖颖彬

LI Na

LI Hao

Related Institution

Department of Gastroenterology, Qingdao Central Hospital of Rehabilitation University

Endoscopy Center, Qingdao Central Hospital of Rehabilitation University

Department of Pathology， Second Affiliated Hospital， Army Medical University （Third Military Medical University）

School of Medicine， Shihezi University

Department of Stomatology， the First Affiliated Hospital of Shihezi University

⁰