Clinical significance of diagnosis and classification of ratio of hsa a_circ_0001708 to IKZF1 for pediatric B-ALI

Lifang FU; Xia HE; Jia LI; Hang ZHANG; Lin ZOU

doi:10.16016/j.1000-5404.202104159

您当前的位置：

首页 >

文章列表页 >

Clinical significance of diagnosis and classification of ratio of hsa a_circ_0001708 to IKZF1 for pediatric B-ALI

Basic Medicine | 更新时间：2026-01-09

- Clinical significance of diagnosis and classification of ratio of hsa a_circ_0001708 to IKZF1 for pediatric B-ALI
- Journal of Third Military Medical University Vol. 43, Issue 20, Pages: 2173-2179(2021)
- 作者机构：
  
  1.400014 重庆，重庆医科大学附属儿童医院临床分子医学中心
  2.625000 四川雅安，雅安市人民医院检验科
  3.610000 成都，成都市第三人民医院实验医学科临床检验部
  4.200062 上海，上海交通大学附属儿童医院临床研究中心
- 作者简介：
- 基金信息：
  
  the General Program of National Natural Science Foundation of China(81570142)
- DOI：10.16016/j.1000-5404.202104159
  CLC： R394.2;R730.43;R733.7
- Received：24 April 2021，
  
  Revised：2021-07-04，
  
  Published：30 October 2021
- 稿件说明：
移动端阅览
Lifang FU, Xia HE, Jia LI, et al. Clinical significance of diagnosis and classification of ratio of hsa a_circ_0001708 to IKZF1 for pediatric B-ALI[J]. Journal of Third Military Medical University, 2021, 43(20): 2173-2179.
DOI：

Lifang FU, Xia HE, Jia LI, et al. Clinical significance of diagnosis and classification of ratio of hsa a_circ_0001708 to IKZF1 for pediatric B-ALI[J]. Journal of Third Military Medical University, 2021, 43(20): 2173-2179. DOI： 10.16016/j.1000-5404.202104159.

摘要

目的

探讨hsa_circ_0001708与IKZF1在儿童急性B淋巴细胞白血病（B-cell acute lymphoblastic leukemia

B-ALL）骨髓单个核细胞（bone marrow mononuclear cells

BMNC）中的拷贝数比值及其在儿童B-ALL诊断及分型中的临床意义。

方法

利用T-A克隆构建包含hsa_circ_0001708反向剪接位点的重组质粒，梯度稀释重组质粒作为标准品建立检测hsa_circ_0001708的绝对RT-qPCR方法。收集2016年1月至2019年1月重庆医科大学附属儿童医院血液科初诊的159例B-ALL患儿骨髓标本为研究对象。对照组为同期就诊的17例非恶性血液病患儿的骨髓标本。采用绝对RT-qPCR方法检测B-ALL患儿和对照患儿BMNC中hsa_circ_0001708和IKZF1的绝对拷贝数，按患者年龄、性别、外周血白细胞数、分子生物学、遗传学、免疫分型、随访期是否复发及随访期末是否生存等临床特点进行分组。回顾性分析hsa_circ_0001708和IKZF1的比值在各组中的表达情况及与以上各因素的相关性。

结果

建立精确定量hsa_circ_0001708拷贝数的绝对RT-qPCR方法。与非血液肿瘤对照患儿相比，hsa_circ_0001708与IKZF1的比值在儿童B-ALL中升高（

0.01），ROC曲线下面积为0.793 6。相关性分析结果提示，hsa_circ_0001708与IKZF1的比值在外周血高白细胞数组、外周血高原始细胞数组、免疫分型pre-B组和细胞遗传学预后较差组中升高（

0.05）。

结论

hsa_circ_0001708与IKZF1的比值对儿童B-ALL有潜在诊断价值，且与B-ALL患儿的MIC分型密切相关。

Abstract

Objective

To investigate the clinical significance of the ratio of hsa_circ_0001708 to IKZF1 in bone marrow mononuclear cells (BMNC) in pediatric B-cell acute lymphoblastic leukemia (B-ALL).

Methods

T-A clone was used to construct the recombinant plasmid containing the back-spliced junction of hsa_circ_0001708

regarding the gradient diluted recombinant plasmid as standard substance to establish the absolute fluorescence quantitative PCR (qPCR) for the detection of hsa_circ_0001708. Bone marrow samples from 159 newly diagnosed pediatric B-ALL patients and 17 patients with non-malignant hemotologic diseases in their matched control group admitted in the Children's Hospital of Chongqing Medical University from January 2016 to January 2019. The copy number of hsa_circ_0001708 and IKZF1 in BMNC of B-ALL children were determined by absolute fluorescence qPCR. The patients were divided into different groups according to their age

gender

peripheral blood leukocyte count

molecular biology

genetics

immune classification

and whether relapse and survive during the follow-up period. The ratio of hsa_circ_0001708 to IKZF1 in different groups and the correlation with these clinical characteristics were analyzed retrospectively.

Results

The absolute RT-qPCR was established that can accurately quantify the copy number of hsa_circ_0001708. The ratio of hsa_circ_0001708 to IKZF1 was significantly increased in pediatric B-ALL (

0.01) when compared with the controls

and the area under the ROC curve (AUC) was 0.793 6. The correlation analysis indicated that the ratio was significant increased in these groups of high leukocyte count

high blast cell count of peripheral blood

pre-B and poor prognosis in cytogenetics (

0.05).

Conclusion

The ratio of hsa_circ_0001708 to IKZF1 has potential diagnostic value and is related to the MIC classification for pediatric B-ALL.

关键词

Keywords

references

SUN W, MALVAR J, SPOSTO R, et al . Outcome of children with multiply relapsed B-cell acute lymphoblastic leukemia: a therapeutic advances in childhood leukemia & lymphoma study[J ] . Leukemia, 2018, 32(11): 2316-2325. DOI: 10.1038/s41375-018-0094-0.

KASTNER P, CHAN S. Role of Ikaros in T-cell acute lymphoblastic leukemia[J]. World J Biol Chem, 2011, 2(6): 108-114. DOI: 10.4331/wjbc.v2.i6.108.

REBOLLO A, SCHMITT C. Ikaros, Aiolos and Helios: transcription regulators and lymphoid malignancies[J]. Immunol Cell Biol, 2003, 81(3): 171-175. DOI: 10.1046/j.1440-1711.2003.01159.x.

MULLIGHAN C G, SU X, ZHANG J, et al . Deletion of IKZF1 and prognosis in acute lymphoblastic leukemia[J ] . N Engl J Med, 2009, 360(5): 470-480. DOI: 10.1056/nejmoa0808253.

付立芳, 邹琳. 环状RNAs在白血病诊治中的研究进展[J]. 临床检验杂志, 2018, 36(10): 767-770. DOI: 10.13602/j.cnki.jcls.2018.10.12.

FU L F, ZOU L. Research progress of circular RNAs in diagnosis and treatment of leukemia[J]. Chin J Clin Lab Sci, 2018, 36(10): 767-770. DOI: 10.13602/j.cnki.jcls.2018.10.12.

付立芳, 张航, 吕文琼, 等. hsa_circ_0004123绝对定量PCR方法的建立及验证[J]. 解放军医学杂志, 2019, 44(11): 906-911. DOI: 10.11855/j.issn.0577-7402.2019.11.02.

FU L F, ZHANG H, LYU W Q, et al . Establishment and validation of hsa_circ_0004123 absolute quantitative PCR method[J ] . Med J Chin PLA, 2019, 44(11): 906-911. DOI: 10.11855/j.issn.0577-7402.2019.11.02.

PUI C H, YANG J J, HUNGER S P, et al . Childhood acute lymphoblastic leukemia: progress through collaboration[J ] . J Clin Oncol, 2015, 33(27): 2938-2948. DOI: 10.1200/JCO.2014.59.1636.

IACOBUCCI I, MULLIGHAN C G. Genetic basis of acute lymphoblastic leukemia[J]. J Clin Oncol, 2017, 35(9): 975-983. DOI: 10.1200/jco.2016.70.7836.

STARY J, ZUNA J, ZALIOVA M. New biological and genetic classification and therapeutically relevant categories in childhood B-cell precursor acute lymphoblastic leukemia[J]. F1000Res, 2018, 7: F1000 Faculty Rev-1569. DOI: 10.12688/f1000research.16074.1.

VROOMAN L M, SILVERMAN L B. Treatment of childhood acute lymphoblastic leukemia: prognostic factors and clinical advances[J]. Curr Hematol Malig Rep, 2016, 11(5): 385-394. DOI: 10.1007/s11899-016-0337-y.

HUNGERS P, MULLIGHAN C G. Redefining ALL classification: toward detecting high-risk ALL and implementing precision medicine[J]. Blood, 2015, 125(26): 3977-3987. DOI: 10.1182/blood-2015-02-580043.

CAI J Y, YU J, ZHU X F, et al . Treatment abandonment in childhood acute lymphoblasticleukaemia in China: a retrospective cohort study of the Chinese Children's Cancer Group[J ] . Arch Dis Child, 2019, 104(6): 522-529. DOI: 10.1136/archdischild-2018-316181.

MULLIGHANC G, GOORHA S, RADTKE I, et al . Genome-wide analysis of genetic alterations in acute lymphoblastic leukaemia[J ] . Nature, 2007, 446(7137): 758-764. DOI: 10.1038/nature05690.

VAIRY S, TRAN T H. IKZF1 alterations in acute lymphoblastic leukemia: The good, the bad and the ugly[J]. Blood Rev, 2020, 44: 100677. DOI: 10.1016/j.blre.2020.100677.

WU Z J, SUN H D, LIU W J, et al . Circ-RPL15: a plasma circular RNA as novel oncogenic driver to promote progression of chronic lymphocytic leukemia[J ] . Leukemia, 2020, 34(3): 919-923. DOI: 10.1038/s41375-019-0594-6.

GUARNERIO J, BEZZI M, JEONG J C, et al . Oncogenic role of fusion-circRNAs derived from cancer-associated chromosomal translocations[J ] . Cell, 2016, 165(2): 289-302. DOI: 10.1016/j.cell.2016.03.020.

LI W, ZHONG C Q, JIAO J, et al . Characterization of hsa_circ_0004277 as a new biomarker for acute myeloid leukemia via circular RNA profile and bioinformatics analysis[J ] . Int J Mol Sci, 2017, 18(3): E597. DOI: 10.3390/ijms18030597..

SUN Y M, WANG W T, ZENG Z C, et al . circMYBL2, acircRNA from MYBL2, regulates FLT3 translation by recruiting PTBP1 to promote FLT3-ITD AML progression[J ] . Blood, 2019, 134(18): 1533-1546. DOI: 10.1182/blood.2019000802.

GAO Y, WANG J F, ZHAO F Q. CIRI: an efficient and unbiased algorithm for de novo circular RNA identification[J]. Genome Biol, 2015, 16: 4. DOI: 10.1186/s13059-014-0571-3.

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Diagnostic value of endoscopic LCPL sign for high-risk intestinal metaplasia in gastric mucosa

Construction of a diagnostic model for chronic mountain sickness among young male migrants to high-altitude areas

Congenital inner ear deformity with spontaneous cerebrospinal fluid leakage: a case series report of 9 children

Application of Hess automatic screen recording system in diagnosis of acquired paralytic strabismus

Rab18 is a potential molecular biomarker for diagnosis and assessing the progression of human gliomas

Related Author

ZHENG Xipeng

PAN Huilin

WU Linyu

HOU Yongying

LIU Qin

MA Qiang

QIN Xiuyuan

LIU Kaijun

Related Institution

Department of Gastroenterology， Chongqing Key Laboratory of Precise Prevention and Treatment of Digestive Malignancies

Department of Pathology， Army Medical Center of PLA/Daping Hospital of Third Military Medical University， Chongqing

First Clinical College of Chongqing Medical University

Department of High Altitude Physiology and Pathology, Faculty of High Altitude Military Medicine， Army Medical University （Third Military Medical University）

Department of Medicine and Hygienic Equipment for High Altitude Region, Faculty of High Altitude Military Medicine， Army Medical University （Third Military Medical University）

⁰