Establishment and preliminary application of a rapid pathogen-specific phenotypic antibiotic susceptibility test for <italic style="font-style: italic">Acinetobacter baumannii</italic>

Dan LUO; Yuliang LI; Bangbi WENG; Qian WANG

doi:10.16016/j.1000-5404.202104143

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Establishment and preliminary application of a rapid pathogen-specific phenotypic antibiotic susceptibility test for Acinetobacter baumannii

Basic Medicine | 更新时间：2026-01-09

- Establishment and preliminary application of a rapid pathogen-specific phenotypic antibiotic susceptibility test for Acinetobacter baumannii
- Journal of Third Military Medical University Vol. 43, Issue 18, Pages: 1775-1782(2021)
- 作者机构：
  
  400038 重庆，陆军军医大学(第三军医大学)第一附属医院药剂科
- 作者简介：
- 基金信息：
  
  the National Natural Science Foundation for Young Scholars of China(81802117);the Project of Outstanding Youth Talent Pool of Army Medical University(XZ-2019-505-012)
- DOI：10.16016/j.1000-5404.202104143
  CLC： R378.7;R446.5;R915
- Received：22 April 2021，
  
  Revised：2021-06-30，
  
  Published：30 September 2021
- 稿件说明：
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Dan LUO, Yuliang LI, Bangbi WENG, et al. Establishment and preliminary application of a rapid pathogen-specific phenotypic antibiotic susceptibility test for Acinetobacter baumannii[J]. Journal of Third Military Medical University, 2021, 43(18): 1775-1782.
DOI：

Dan LUO, Yuliang LI, Bangbi WENG, et al. Establishment and preliminary application of a rapid pathogen-specific phenotypic antibiotic susceptibility test for Acinetobacter baumannii[J]. Journal of Third Military Medical University, 2021, 43(18): 1775-1782. DOI： 10.16016/j.1000-5404.202104143.

摘要

目的

利用表面活性剂-叠氮溴化丙锭(propidium monoazide

PMAxx)-qPCR反应体系对鲍曼不动杆菌进行快速表型药敏预测。

方法

依次对加入反应体系中表面活性剂的种类和最适浓度、叠氮溴化丙锭曝光时间和浓度进行优化；采用qPCR检测鲍曼不动杆菌特异性鉴别基因(blaOXA-51)拷贝数；采用抗菌药物-细菌共反应体系测定鲍曼不动杆菌对抗菌药物的敏感性。

结果

当表面活性剂月桂酰基甘氨酸钠浓度为0.2%，叠氮溴化丙锭最佳曝光时间和浓度为5 min和20 μmol/L时，能有效抑制死细胞DNA扩增，且不影响活细胞。基于此建立的表型药敏预测模型能快速(2 h内)和准确地预测鲍曼不动杆菌对多西环素及左氧氟沙星的敏感性(

0.01)，对头孢哌酮/舒巴坦的敏感性更可靠的预测需超过2 h。

结论

基于月桂酰基甘氨酸钠-PMAxx-qPCR体系的快速表型药敏预测新方法可在短时间内为鲍曼不动杆菌感染的治疗提供药敏依据，以指导其临床精准用药。

Abstract

Objective

To establish a rapid phenotypic antibiotic susceptibility testing of

Acinetobacter baumannii

based on surfactant-propidium monoazide (PMAxx)-qPCR reaction system.

Methods

The type and optimal concentration of surfactant

exposure time and concentration of PMAxx were optimized successively. The copies of genomic DNA of

Acinetobacter baumannii

specific discriminant gene (blaOXA-51) was detected by qPCR. The susceptibility of

Acinetobacter baumannii

to antibiotics was determined by the antibiotic-bacterial co-reaction system.

Results

The Results indicated that 0.2% sodium lauroylsarcosinate and 20 μmol/L PMAxx af

ter 5 min exposure effectively inhibited the DNA amplification of dead cells but had no effect on that of live cells. The phenotypic antibiotic susceptibility testing model based on this study could quickly (within 2 h) and accurately predict the susceptibility of

Acinetobacter baumannii

to doxycycline and levofloxacin (P

0.01)

and the reliable susceptibility of cefoperazone/sulbactam was predicted more than 2 h.

Conclusion

Our phenotypic antibiotic susceptibility test based on the sodium lauroylsarcosinate-PMAxx-qPCR system provides a basis for the treatment of

Acinetobacter baumannii

within a short time

and guides the clinical precision medication.

关键词

Keywords

references

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Establishment and preliminary application of a rapid pathogen-specific phenotypic antibiotic susceptibility test for Acinetobacter baumannii

DOI：10.16016/j.1000-5404.202104143

摘要

Abstract

关键词

Keywords

references