Expression and clinical significance of SnoN, TGF-β1 and Smad4 in endometrium of patients with severe intrauterine adhesion

Boping YANG; Jianxin GUO; Hao WANG; Yiping YUAN; Yugang CHI; Li LI

doi:10.16016/j.1000-5404.202103150

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Expression and clinical significance of SnoN, TGF-β1 and Smad4 in endometrium of patients with severe intrauterine adhesion

更新时间：2026-01-09

- Expression and clinical significance of SnoN, TGF-β1 and Smad4 in endometrium of patients with severe intrauterine adhesion
- Journal of Third Military Medical University Vol. 43, Issue 17, Pages: 1667-1672(2021)
- 作者机构：
- 作者简介：
- 基金信息：
  
  the General Program of National Natural Science Foundation of Chongqing(cstc2020jcyj-msxmX0403);the General Program of Chongqing Health Center for Women and Children(2019YJMS03)
- DOI：10.16016/j.1000-5404.202103150
  CLC：
- Received：18 March 2021，
  
  Published：15 September 2021
- 稿件说明：
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Boping YANG, Jianxin GUO, Hao WANG, et al. Expression and clinical significance of SnoN, TGF-β1 and Smad4 in endometrium of patients with severe intrauterine adhesion[J]. Journal of Third Military Medical University, 2021, 43(17): 1667-1672.
DOI：

Boping YANG, Jianxin GUO, Hao WANG, et al. Expression and clinical significance of SnoN, TGF-β1 and Smad4 in endometrium of patients with severe intrauterine adhesion[J]. Journal of Third Military Medical University, 2021, 43(17): 1667-1672. DOI： 10.16016/j.1000-5404.202103150.

摘要

目的

探讨核转录共抑制因子(ski-related novel protein N，SnoN)在宫腔粘连(intrauterine adhesions，IUA)发病中的作用，及其与转化生长因子β1 (transforming growth factor-β1，TGF-β1)、Smad4关系。

方法

收集重度宫腔粘连(sIUA组)和宫腔无病变的子宫纵隔患者(对照组)宫内膜组织各20例，通过免疫组化、Western blot、RT-qPCR检测子宫内膜组织中SnoN、TGF-β1、Smad4的表达水平，并应用Spearman等级相关分析SnoN、TGF-β1、Smad4之间的相关性，探讨其与宫腔粘连发病的关系。

结果

sIUA组患者宫内膜组织中TGF-β1、Smad4表达水平明显高于对照组(

0.01)，而SnoN表达水平明显低于对照组(

0.01)。在重度宫腔粘连宫内膜组织中，TGF-β1与Smad4的蛋白表达水平呈正相关(

=0.468 9，

0.05)，而TGF-β1与SnoN的蛋白水平呈负相关(

=－0.483 5，

0.05)。

结论

SnoN可能通过TGF-β1/Smad4信号通路参与宫腔粘连的发病。

Abstract

Objective

To investigate the role of ski-related novel protein N (SnoN) in the pathogenesis of intrauterine adhesion (IUA) and its association with transforming growth factor-β1 (TGF-β1) and Smad4 in endometrial tissue in IUA patients.

Methods

Endometrial tissues of 20 severe IUA patients (sIUA group) and 20 healthy women with uterine septum (control group) were collected from our 2 departments during January 2019 and October 2020. Immunohistochemical assay

Western blotting and reverse transcription quantitative polymerase chain reaction (RT-qPCR) were used to detect the expression of SnoN

TGF-β1 and Smad4 in the endometrial tissue of the control and sIUA groups. The correlations of their expression levels in the endometrial tissues were analyzed with Spearman rank correlation analysis to investigate the association of the levels and IUA.

Results

The expression levels of TGF-β1 and Smad4 were significantly higher

while that of SnoN was obviously lower in the sIUA group than the control group (all

0.01). In the endometrial tissues from the sIUA group

the expression of Smad4 was positively correlated with that of TGF-β1 (

=0.468 9

0.05). However

the TGF-β1 expression was negatively correlated with that of SnoN (

=-0.483 5

0.05).

Conclusion

SnoN may be involved in the pathogenesis of IUA

via

the TGF-β1/Smad4 signal pathway.

关键词

Keywords

references

The American Fertility Society Classifications of adnexal adhesions, distal tubal occlusion, tubal occlusion secondary to tubal ligation, tubal pregnancies, müllerian anomalies and intrauterine adhesions Fertil Steril 1988 49 6 944 955 10.1016/s0015-0282(16)59942-7 The American Fertility Society. Classifications of adnexal adhesions, distal tubal occlusion, tubal occlusion secondary to tubal ligation, tubal pregnancies, müllerian anomalies and intrauterine adhesions[J]. Fertil Steril, 1988, 49(6): 944-955. DOI: 10.1016/s0015-0282(16)59942-7.

ZHANG L XU W H FU X H Therapeutic role of granulocyte colony-stimulating factor (G-CSF) for infertile women under in vitro fertilization and embryo transfer (IVF-ET) treatment: a meta-analysis Arch Gynecol Obstet 2018 298 5 861 871 10.1007/s00404-018-4892-4 ZHANG L, XU W H, FU X H, et al . Therapeutic role of granulocyte colony-stimulating factor (G-CSF) for infertile women under in vitro fertilization and embryo transfer (IVF-ET) treatment: a meta-analysis[J ] . Arch Gynecol Obstet, 2018, 298(5): 861-871. DOI: 10.1007/s00404-018-4892-4.

PAN D ZHU Q LUO K SnoN functions as a tumour suppressor by inducing premature senescence EMBO J 2009 28 22 3500 3513 10.1038/emboj.2009.250 PAN D, ZHU Q, LUO K. SnoN functions as a tumour suppressor by inducing premature senescence[J]. EMBO J, 2009, 28(22): 3500-3513. DOI: 10.1038/emboj.2009.250.

罗丽兰不孕与不育北京人民卫生出版社 2009 262 263 罗丽兰. 不孕与不育[M]. 北京: 人民卫生出版社, 2009: 262-263.

陈坤菊史小荣代晓琴宫腔粘连的预防方法研究进展国际妇产科学杂志 2017 44 5 576 580 10.3969/j.issn.1674-1870.2017.05.022 陈坤菊, 史小荣, 代晓琴. 宫腔粘连的预防方法研究进展[J]. 国际妇产科学杂志, 2017, 44(5): 576-580. DOI: 10.3969/j.issn.1674-1870.2017.05.022.

HILL C S Transcriptional control by the SMADs Cold Spring Harb Perspect Biol 2016 8 10 a022079 10.1101/cshperspect.a022079 HILL C S. Transcriptional control by the SMADs[J]. Cold Spring Harb Perspect Biol, 2016, 8(10): a022079. DOI: 10.1101/cshperspect.a022079.

WALLDÉN K NYMAN T HÄLLBERG B M SnoN stabilizes the SMAD3/SMAD4 protein complex Sci Rep 2017 7 46370 10.1038/srep46370 WALLDÉN K, NYMAN T, HÄLLBERG B M. SnoN stabilizes the SMAD3/SMAD4 protein complex[J]. Sci Rep, 2017, 7: 46370. DOI: 10.1038/srep46370.

WALTON K L JOHNSON K E HARRISON C A Targeting TGF-β mediated SMAD signaling for the prevention of fibrosis Front Pharmacol 2017 8 461 10.3389/fphar.2017.00461 WALTON K L, JOHNSON K E, HARRISON C A. Targeting TGF-β mediated SMAD signaling for the prevention of fibrosis[J]. Front Pharmacol, 2017, 8: 461. DOI: 10.3389/fphar.2017.00461.

LI J X CEN B H CHEN S P MicroRNA-29b inhibits TGF-β1-induced fibrosis via regulation of the TGF-β1/Smad pathway in primary human endometrial stromal cells Mol Med Rep 2016 13 5 4229 4237 10.3892/mmr.2016.5062 LI J X, CEN B H, CHEN S P, et al . MicroRNA-29b inhibits TGF-β1-induced fibrosis via regulation of the TGF-β1/Smad pathway in primary human endometrial stromal cells[J ] . Mol Med Rep, 2016, 13(5): 4229-4237. DOI: 10.3892/mmr.2016.5062.

李灿宇党秋红刘欢欢 TGF-β1和smad4在中重度宫腔粘连组织中的表达及临床意义中国妇产科临床杂志 2018 19 4 347 349 10.13390/j.issn.1672-1861.2018.04.016 李灿宇, 党秋红, 刘欢欢, 等. TGF-β1和smad4在中重度宫腔粘连组织中的表达及临床意义[J]. 中国妇产科临床杂志, 2018, 19(4): 347-349. DOI: 10.13390/j.issn.1672-1861.2018.04.016.

付青卢秀琴王延明宫腔粘连分子机制的研究进展临床与实验病理学杂志 2019 35 9 1079 1081 10.13315/j.cnki.cjcep.2019.09.015 付青, 卢秀琴, 王延明, 等. 宫腔粘连分子机制的研究进展[J]. 临床与实验病理学杂志, 2019, 35(9): 1079-1081. DOI: 10.13315/j.cnki.cjcep.2019.09.015.

LIU L R SHI M J WANG Y Y SnoN upregulation ameliorates renal fibrosis in diabetic nephropathy PLoS ONE 2017 12 3 e0174471 10.1371/journal.pone.0174471 LIU L R, SHI M J, WANG Y Y, et al . SnoN upregulation ameliorates renal fibrosis in diabetic nephropathy[J ] . PLoS ONE, 2017, 12(3): e0174471. DOI: 10.1371/journal.pone.0174471.

ZEGLINSKI M R HNATOWICH M JASSAL D S SnoN as a novel negative regulator of TGF-β/Smad signaling: a target for tailoring organ fibrosis Am J Physiol Heart Circ Physiol 2015 308 2 H75 H82 10.1152/ajpheart.00453.2014 ZEGLINSKI M R, HNATOWICH M, JASSAL D S, et al . SnoN as a novel negative regulator of TGF-β/Smad signaling: a target for tailoring organ fibrosis[J ] . Am J Physiol Heart Circ Physiol, 2015, 308(2): H75-H82. DOI: 10.1152/ajpheart.00453.2014.

TECALCO-CRUZ A C RÍOS-L®PEZ D G VÁZQUEZ-VICTORIO G Transcriptional cofactors Ski and SnoN are major regulators of the TGF-β/Smad signaling pathway in health and disease Signal Transduct Target Ther 2018 3 15 10.1038/s41392-018-0015-8 TECALCO-CRUZ A C, RÍOS-L®PEZ D G, VÁZQUEZ-VICTORIO G, et al . Transcription al cofactors Ski and SnoN are major regulators of the TGF-β/Smad signaling pathway in health and disease[J ] . Signal Transduct Target Ther, 2018, 3: 15. DOI: 10.1038/s41392-018-0015-8.

AI K ZHU X KANG Y miR-130a-3p inhibition protects against renal fibrosis in vitro via the TGF-β1/Smad pathway by targeting SnoN Exp Mol Pathol 2020 112 104358 10.1016/j.yexmp.2019.104358 AI K, ZHU X, KANG Y, et al . miR-130a-3p inhibition protects against renal fibrosis in vitro via the TGF-β1/Smad pathway by targeting SnoN[J ] . Exp Mol Pathol, 2020, 112: 104358. DOI: 10.1016/j.yexmp.2019.104358.

张秀莉李万成 SnoN在肺纤维化TGF-β1/smad通路中的作用生命的化学 2018 38 2 236 240 10.13488/j.smhx.20180209 张秀莉, 李万成. SnoN在肺纤维化TGF-β1/smad通路中的作用[J]. 生命的化学, 2018, 38(2): 236-240. DOI: 10.13488/j.smhx.20180209.

LIU L WANG Y YAN R Oxymatrine inhibits renal tubular EMT induced by high glucose via upregulation of SnoN and inhibition of TGF-β1/smad signaling pathway PLoS ONE 2016 11 3 e0151986 10.1371/journal.pone.0151986 LIU L, WANG Y, YAN R, et al . Oxymatrine inhibits renal tubular EMT induced by high glucose via upregulation of SnoN and inhibition of TGF-β1/smad signaling pathway[J ] . PLoS ONE, 2016, 11(3): e0151986. DOI:10.1371/journal.pone.0151986.

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