Effects of <italic style="font-style: italic">Fscb</italic>/<italic style="font-style: italic">Cabyr</italic> double gene knockout on reproductive capacity in male mice

Zhongtai LI; Yanfeng LI; Jinyi LIU; Yong ZHANG; Jun ZHANG; Gang BI

doi:10.16016/j.1000-5404.202102131

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Effects of Fscb/Cabyr double gene knockout on reproductive capacity in male mice

Basic Medicine | 更新时间：2026-02-13

- Effects of Fscb/Cabyr double gene knockout on reproductive capacity in male mice
- Journal of Third Military Medical University Vol. 43, Issue 16, Pages: 1521-1526(2021)
- 作者机构：
  
  1.400042 重庆，陆军军医大学(第三军医大学)大坪医院泌尿外科
  2.400038 重庆，陆军军医大学(第三军医大学)预防医学院毒理学研究所
  3.463000 河南驻马店，联勤保障部队第 990 医院泌尿外科
- 作者简介：
- 基金信息：
  
  the General Program of National Natural Science Foundation of China(81571491)
- DOI：10.16016/j.1000-5404.202102131
  CLC： R321.1;R329.2;R698.2
- Received：24 February 2021，
  
  Revised：2021-05-06，
  
  Published：30 August 2021
- 稿件说明：
移动端阅览
Zhongtai LI, Yanfeng LI, Jinyi LIU, et al. Effects of Fscb/Cabyr double gene knockout on reproductive capacity in male mice[J]. Journal of Third Military Medical University, 2021, 43(16): 1521-1526.
DOI：

Zhongtai LI, Yanfeng LI, Jinyi LIU, et al. Effects of Fscb/Cabyr double gene knockout on reproductive capacity in male mice[J]. Journal of Third Military Medical University, 2021, 43(16): 1521-1526. DOI： 10.16016/j.1000-5404.202102131.

摘要

目的

观察

Fscb

Cabyr

双基因和单基因敲除雄鼠繁殖能力，探讨CABYR蛋白及与FSCB的复合物对于维持精子授精能力

的价值及其发挥生理功能的可能机制。

方法

取

Fscb

Cabyr

(--/--)双基因敲除纯合子雄鼠、

Fscb

Cabyr

(++/--)单基因敲除雄鼠及野生型雄鼠分别与同代野生型雌鼠交配，观察各组雌鼠怀孕、产仔情况；应用Fluo-4 AM钙离子荧光探针对不同基因型雄鼠精子进行标记，荧光显微镜下观察并应用酶标仪检测各基因型小鼠精子中钙离子水平；Western blot检测各基因型小鼠精子中各种CATSPER通道蛋白的表达情况。

结果

所有

Fscb

Cabyr

(--/--)双基因敲除与

Cabyr

单基因敲除纯合子雄鼠繁殖能力完全丧失，均未能使任何雌鼠怀孕和产仔。与野生型小鼠相比，

Fscb

Cabyr

(--/--)及

Fscb

Cabyr

(++/--)基因型雄鼠精子内钙离子浓度显著降低(

＜0.000 1)；

Fscb

Cabyr

(--/--)型小鼠精子中的CATSPER-1蛋白表达显著减少。

Fscb

Cabyr

(--/--)、

Fscb

Cabyr

(++/--)型小鼠精子中的CATSPER-4蛋白表达显著减低。

结论

雄鼠精子CABYR及FSCB/CABYR蛋白复合物的缺失导致雄鼠繁殖功能的丧失，可能是通过影响精子内的钙通道蛋白CATSPER-1和CATSPER-4的表达和钙离子浓度水平，从而影响精子的运动功能而实现的。

Abstract

Objective

To evaluate the value of CABYR protein and its complex with FSCB in maintaining sperm fertilization ability and investigate the possible mechanism of physiological function of the coruplex by observing the natural fertility of

Fscb

Cabyr

double gene knockout and

Cabyr

single gene knockout male mice.

Methods

Fscb

Cabyr

(--/--) double gene knockout male mice

Fscb

Cabyr

(++/--) single gene knockout male mice and wild background male mice were mated with wild-type female mice respectively

and the pregnancy and litter production of female mice in each group were observed. The sperm of male mice with different genotypes were labeled with Fluo-4 AM calcium fluorescent probe

in vitro

and observed under fluorescence microscope

and the level of calcium ion in the sperm were detected by ELISA;

The expression of CATSPER channel proteins in sperm of mice with different genotypes was measured by Western blotting.

Results

All the male mice with

Fscb

Cabyr

(--/--) double gene knockout and

Fscb

Cabyr

(++/--) single gene knockout lost their reproductive capacity completely

and failed to make any female mice pregnant and give birth. Compared with the wild-type male mice

the sperm calcium ion concentration of

Fscb

Cabyr

(--/--) and

Fscb

Cabyr

(++/--) male mice was decreased significantly (

0.000 1); The expression of CATSPER-1 protein in the sperm of

Fscb

Cabyr

(--/--) mice was decreased obviously. The expression of CATSPER-4 in the sperm of

Fscb

Cabyr

(--/--) and

Fscb

Cabyr

(++/--) mice were notably decreased.

Conclusion

The deletion of either CABYR or FSCB/CABYR protein complex in male sperm leads to the loss of reproductive function in the male mice

which may be realized by affecting the expression of calcium channel proteins CATSPER-1 and CATSPER-4

concentration of calcium ions in sperm

and then motility function of sperm.

关键词

Keywords

references

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⁰

Effects of Fscb/Cabyr double gene knockout on reproductive capacity in male mice

DOI：10.16016/j.1000-5404.202102131

摘要

Abstract

关键词

Keywords

references