Effects of uremic toxin p-cresol on oxidative stress in human coronary artery endothelial cells

Li LI; Jing LI; Fahuan YUAN; Xiaoli LI

doi:10.16016/j.1000-5404.202102059

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Effects of uremic toxin p-cresol on oxidative stress in human coronary artery endothelial cells

Basic Medicine | 更新时间：2026-01-09

- Effects of uremic toxin p-cresol on oxidative stress in human coronary artery endothelial cells
- Journal of Third Military Medical University Vol. 43, Issue 16, Pages: 1543-1549(2021)
- 作者机构：
  
  1.541001 广西桂林，桂林医学院附属医院肾内科
  2.541001 广西桂林，中国人民解放军联勤保障部队第九二四医院心内科
  3.400038 重庆，陆军军医大学(第三军医大学)第二附属医院肾内科
- 作者简介：
- 基金信息：
  
  the General Program of Natural Science Foundation of Guangxi Zhuang Autonomous Region(2018GXNSFAA138119)
- DOI：10.16016/j.1000-5404.202102059
  CLC： R322.121;R329.25;R914.4
- Received：28 February 2021，
  
  Revised：2021-03-30，
  
  Published：30 August 2021
- 稿件说明：
移动端阅览
Li LI, Jing LI, Fahuan YUAN, et al. Effects of uremic toxin p-cresol on oxidative stress in human coronary artery endothelial cells[J]. Journal of Third Military Medical University, 2021, 43(16): 1543-1549.
DOI：

Li LI, Jing LI, Fahuan YUAN, et al. Effects of uremic toxin p-cresol on oxidative stress in human coronary artery endothelial cells[J]. Journal of Third Military Medical University, 2021, 43(16): 1543-1549. DOI： 10.16016/j.1000-5404.202102059.

摘要

目的

探讨尿毒症毒素对甲酚对人冠状动脉内皮细胞(human coronary artery endothelial cells

HCAECs)氧化应激的影响。

方法

体外培养HCAECs，将不同浓度的游离对甲酚或者不同浓度与4%白蛋白结合的对甲酚，分别作用HCAECs 24、48 h，采用MTT检测各组细胞增殖，特异性ELISA试剂盒检测细胞活性氧自由基(reactive oxygen species，ROS)水平，实时定量聚合酶链反应(real-time PCR)检测细胞中还原型烟酰胺腺嘌呤二核苷酸磷酸(nicotinamide adenine dinucleotide phosphate oxidase，NADPH)氧化酶NOX2和NOX4的mRNA表达，Western blot检测细胞中NOX2、NOX4、p-p38和p-38的蛋白表达。

结果

游离的和蛋白结合的对甲酚显著抑制HCAECs的增殖(

＜0.05)，增加HCAECs中ROS的含量(

＜0.05)、NOX2、NOX4 mRNA表达(

＜0.05)和NOX2、NOX4、p-p38蛋白表达(

＜0.05)。

结论

游离的和蛋白结合的对甲酚通过调控MAPKs信号通路诱导HCAECs氧化应激，抑制HCAECs增殖。

Abstract

Objective

To investigate the effects of uremic toxin p-cresol (PC) on the oxidative stress of human coronary artery endothelial cells (HCAECs).

Methods

HCAECs were cultured

in vitro

and treated with different concentrations of free PC alone or combined with 4% albumin for 24 and 48 h

respectively. Cell proliferation was detected by MTT assay; The production of cellular reactive oxygen free radicals (reactive oxygen species

ROS) was detected with specific ELISA kit; The mRNA levels of NADPH oxidases NOX2 and NOX4 were measured with real-time PCR; The protein levels of NOX2

NOX4

p-p38 and p-38 were tested with Western blotting.

Results

Free and protein-bound PC significantly inhibited the proliferation of HCAECs (

0.05)

increased the production of ROS (

0.05)

enhanced the mRNA expression levels of NOX2 and NOX4 (

0.05)

and up-regulated the protein levels of NOX2

NOX4 and p-p38 in HCAECs (

0.05).

Conclusion

The free and protein-bound PC induces oxidative stress and inhibits the proliferation of HCAECs by regulating the MAPKs signaling pathway.

关键词

Keywords

references

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