Effects of high-frequency repetitive transcranial magnetic stimulation on remyelination in a rat model of focal cerebral ischemia

Ruolan WANG; Youhong XIE; Ning YAN; Jiankun MU; Zhou HE

doi:10.16016/j.1000-5404.202101235

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Effects of high-frequency repetitive transcranial magnetic stimulation on remyelination in a rat model of focal cerebral ischemia

Neurosciences | 更新时间：2026-01-09

- Effects of high-frequency repetitive transcranial magnetic stimulation on remyelination in a rat model of focal cerebral ischemia
- Journal of Third Military Medical University Vol. 43, Issue 14, Pages: 1304-1311(2021)
- 作者机构：
  
  1.400050 重庆，重庆医科大学附属康复医院神经康复科
  2.401331 重庆，重庆医科大学附属大学城医院神经内科
- 作者简介：
- 基金信息：
  
  the Key Project of Medical Research of Chongqing Health and Family Planning Commission(2017ZDXM022)
- DOI：10.16016/j.1000-5404.202101235
  CLC： R454.1;R741.05;R743.34
- Received：27 January 2021，
  
  Revised：2021-03-03，
  
  Published：30 July 2021
- 稿件说明：
移动端阅览
Ruolan WANG, Youhong XIE, Ning YAN, et al. Effects of high-frequency repetitive transcranial magnetic stimulation on remyelination in a rat model of focal cerebral ischemia[J]. Journal of Third Military Medical University, 2021, 43(14): 1304-1311.
DOI：

Ruolan WANG, Youhong XIE, Ning YAN, et al. Effects of high-frequency repetitive transcranial magnetic stimulation on remyelination in a rat model of focal cerebral ischemia[J]. Journal of Third Military Medical University, 2021, 43(14): 1304-1311. DOI： 10.16016/j.1000-5404.202101235.

摘要

目的

探讨大鼠局灶性脑缺血后介入高频重复经颅磁刺激(repetitive transcranial magnetic stimulation

rTMS)治疗对大鼠运动功能的影响及对少突胶质前体细胞(OPC)、少突胶质细胞转录因子(Olig1)、髓鞘碱性蛋白(MBP)、NG2细胞表达的影响。

方法

将63只SPF级成年雄性Sprague-Dawley大鼠采用随机抽样法分为模型组、rTMS组、假手术组，每组21只，3组分别按术后1、3、10 d各分7只。模型组、rTMS组使用线栓法制备短暂性大脑中动脉栓塞(tMCAO)模型，模型组建模成功后自然恢复，rTMS组建模成功后每24 h介入高频rTMS治疗，干预10 d。使用改良大鼠神经功能缺损评分评定rTMS对大鼠运动功能影响。各组分别于建模成功后第1、3、10天取脑，通过对NG2、髓鞘碱性蛋白(MBP)进行免疫组织化染色，观察缺血侧纹状体中少突胶质前体细胞的增殖以及髓鞘损伤程度；Western blot检测Olig1、MBP、NG2的表达量。

结果

rTMS降低tMCAO后的神经功能缺损评分，治疗后rTMS组的神经功能缺损评分低于模型组(

0.05)；rTMS促进tMCAO后OPC的增殖和分化，其中rTMS组的NG2表达在第1、3、10天均高于模型组(

0.05)；Olig1表达在第3、10天高于模型组(

0.05)。rTMS促进脑组织缺血后的髓鞘修复，模

型组MBP表达始终低于假手术组(

0.05)，治疗后rTMS组MBP表达在10 d时高于模型组(

0.05)。

结论

缺血性脑卒中后急性期rTMS治疗可促进OPC增殖和髓鞘修复。

Abstract

Objective

To explore the effects of high-frequency repetitive transcranial magnetic stimulation (rTMS) on the motor function

proliferation and differentiation of oligodendrocyte precursor cells (OPC)

and expression of oligodendrocyte transcription factor 1 (Olig1)

myelin basic protein (MBP) and NG2 in the striatum of rats after focal cerebral ischemia.

Methods

Sixty-three adult male SPF Sprague-Dawley (SD) rats were randomly divided into model group

rTMS group

and sham group

with 21 rats in each group. Transient middle cerebral artery embolism (tMCAO) model was prepared by thread embolization in the model group and rTMS group. Subsequently

the model group recovered naturally without any intervention

while the rTMS group received high frequency rTMS treatment every 24 h for 10 d. Each group was further assigned into 7 rats at the time points of days 1

and 10 postoperatively

and modified neurological severity score (mNSS) was used to evaluate the motor function of the rats. Then the brains of each group were taken out for further analyses. Immunohistochemical staining was performed to observe the expression of NG2 and MBP in the ischemic striatum to evaluate the proliferation of OPC and severity of myelin damage. Western blotting was adopted to detect the expression of Olig1

MBP

and NG2 in the ischemic striatum.

Results

rTMS treatment significantly reduced the mNSS of tMCAO rats

with lower score in the rTMS group than in the model group (

0.05); It also promoted the proliferation and differentiation of OPC in tMCAO rats

which presented as a higher level of NG2 in the rTMS group than in the model group on days 1

3 and 10 postoperatively (

0.05)

as well as a higher level of Olig1 on days 3 and 10 (

0.05). In addition

although MBP expression in the model group was consistently lower than that in the sham group (

0.05)

the level was higher in the rTMS group than the model group on day 10 (

0.05)

suggesting that rTMS also promoted the repair of myelin sheath after cerebral ischemia.

Conclusion

Application of high-frequency rTMS can promote OPC proliferation and myelin repair in the acute phase of post-ischemic stroke.

关键词

Keywords

references

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