Pericyte-derived microvesicles protects septic rats against lung injury

Hongchen WANG; Henan ZHOU; Danyang ZHENG; Mingying XUE; Kunlun TIAN; Yue WU; Xiaoyong PENG; Liangming LIU; Tao LI

doi:10.16016/j.1000-5404.202101031

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Pericyte-derived microvesicles protects septic rats against lung injury

Basic Medicine | 更新时间：2026-01-09

- Pericyte-derived microvesicles protects septic rats against lung injury
- Journal of Third Military Medical University Vol. 43, Issue 13, Pages: 1185-1191(2021)
- 作者机构：
  
  400042 重庆，陆军特色医学中心野战外科研究部战伤休克与输血研究室，创伤、烧伤与复合伤国家重点实验室
- 作者简介：
- 基金信息：
  
  the Key Program of National Natural Science Foundation of China(81830065)
- DOI：10.16016/j.1000-5404.202101031
  CLC： R322.12;R563;R631
- Received：04 January 2021，
  
  Revised：2021-01-21，
  
  Published：15 July 2021
- 稿件说明：
移动端阅览
Hongchen WANG, Henan ZHOU, Danyang ZHENG, et al. Pericyte-derived microvesicles protects septic rats against lung injury[J]. Journal of Third Military Medical University, 2021, 43(13): 1185-1191.
DOI：

Hongchen WANG, Henan ZHOU, Danyang ZHENG, et al. Pericyte-derived microvesicles protects septic rats against lung injury[J]. Journal of Third Military Medical University, 2021, 43(13): 1185-1191. DOI： 10.16016/j.1000-5404.202101031.

摘要

目的

探讨周细胞(pericyte，PC)分泌的微囊泡(microvesicle，MV)对脓毒症大鼠肺损伤的保护作用。

方法

利用差速离心法提取周细胞微囊泡(PC derived MV，PC-MV)，并采用透射电镜和流式细胞仪进行鉴定。将40只SD大鼠(雌雄不拘，12~14周龄，体质量约220 g)按随机数字表法分为4组(

=10): 正常组、PC-MV对照组(正常小鼠尾静脉注射PC-MV，1×10

/只)、脓毒症组和PC-MV治疗组(脓毒症模型大鼠尾静脉注射PC-MV，1×10

/只)。检测大鼠的肺湿干质量比和肺组织伊文思蓝渗出量，观察PC-MV对脓毒症大鼠肺血管通透性的作用；制备肺病理切片并检测动脉血气指标，观察PC-MV对肺组织功能的作用；检测大鼠血清炎性因子水平，观察PC-MV对脓毒症炎症反应的作用。

结果

与正常组相比，脓毒症组大鼠肺功能明显受损，表现为肺组织结构破坏，大量红细胞及炎性细胞渗出，动脉血氧分压(PaO

)下降33.6%，动脉血二氧化碳分压(PaCO

)升高47.5%；脓毒症大鼠肺血管通透性显著增加，表现为肺湿干质量比增加，肺对伊文思蓝透过率增加；脓毒症大鼠炎性因子TNF-α、IL-6水平升高(

0.01)。输注外源性PC-MV可改善肺病理结构，恢复动脉血气(与正常组相比，PC-MV治疗组PaO

下降17.7%，PaCO

升高26.9%)，减轻炎性因子渗出(与脓毒症组相比，PC-MV治疗组炎性因子TNF-α水平下降10.7%，IL-6水平下降23.9%)，显著保护脓毒症大鼠肺功能。同时PC-MV可减轻脓毒症后肺血管渗漏，对肺血管通透性有明显的保护作用。

结论

PC-MV对脓毒症大鼠肺损伤有明显的保护作用。

Abstract

Objective

To investigate the protective effect of pericyte (PC)-derived microvesicle (MV) on lung injury in septic rats.

Methods

PC-MV was harvested by differential centrifugation and identified by transmission electron microscopy (TEM) and flow cytometry (FCM). Forty SD rats of either sex (12~14 weeks old

about 220 g) were randomly divided into 4 groups (

=10): normal group

PC-MV control group (normal rats with PC-MV injection)

sepsis group (cecal ligation puncture

LP)

and PC-MV treatment group(septic rats with PC-MV injection). The effects of PC-MV (1×10

per rat) on pulmonary vascular permeability in septic rats were observed by measuring the wet/dry weight ratio of lung and Evans blue dye exudation in lung tissue

those on pulmonary tissue function were determined by lung pathological sections and arterial blood gas analysis

and those on inflammation were observed by detecting the levels of inflammatory factors.

Results

Compared with the normal group

the pulmonary function was obviously impaired in the septic rats

which was manifested as destruction of lung tissue structure

exudation of a large number of red blood cells and inflammatory cells

and decrease of oxygen partial pressure (PaO

33.6%) and increase of carbon dioxide partial pressure in the arteries (PaCO

47.5%). In septic rats

pulmonary vascular permeability was significantly increased

which was manifested as increment of lung wet/dry weight ratio

increment of Evans blue permeability in the lung

and increased levels of TNF-α and IL-6 (

0.01). Exogenous PC-MV improved pulmonary structure

restored the arterial blood gas (compared with the normal group

PaO

: decreased by 17.7%; PaCO

: increased by 26.9%)

reduced inflammatory factor exfiltration (compared with the sepsis group

TNF-α level: decreased by 10.7%; IL-6 level: decreased by 23.9%) in PC-MV treatment group when compared with the sepsis group)

and significantly protected pulmonary function of septic rats.

Conclusion

PC-MV has a protective effect on lung injury in septic rats.

关键词

Keywords

references

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