Artesunate reduces IL-6 production in primary mouse hepatocytes induced by conjugated bile acids through inhibiting p-p65 signaling pathway

Min LIAO; Xuan LI; Liangjun ZHANG; Pan SHI; Jin CHAI

doi:10.16016/j.1000-5404.202012225

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Artesunate reduces IL-6 production in primary mouse hepatocytes induced by conjugated bile acids through inhibiting p-p65 signaling pathway

Basic Medicine | 更新时间：2026-01-09

- Artesunate reduces IL-6 production in primary mouse hepatocytes induced by conjugated bile acids through inhibiting p-p65 signaling pathway
- Journal of Third Military Medical University Vol. 43, Issue 12, Pages: 1133-1139(2021)
- 作者机构：
  
  400038 重庆陆军军医大学第一附属医院消化内科胆汁淤积性肝病中心
- 作者简介：
- 基金信息：
  
  the National Natural Science Foundation for Distinguished Young Scholars of China(81922012)
- DOI：10.16016/j.1000-5404.202012225
  CLC： R654.3;R654.4;R931.6
- Received：28 December 2020，
  
  Revised：2021-03-29，
  
  Published：30 June 2021
- 稿件说明：
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Min LIAO, Xuan LI, Liangjun ZHANG, et al. Artesunate reduces IL-6 production in primary mouse hepatocytes induced by conjugated bile acids through inhibiting p-p65 signaling pathway[J]. Journal of Third Military Medical University, 2021, 43(12): 1133-1139.
DOI：

Min LIAO, Xuan LI, Liangjun ZHANG, et al. Artesunate reduces IL-6 production in primary mouse hepatocytes induced by conjugated bile acids through inhibiting p-p65 signaling pathway[J]. Journal of Third Military Medical University, 2021, 43(12): 1133-1139. DOI： 10.16016/j.1000-5404.202012225.

摘要

目的

探讨青蒿琥酯(Artesunate

AS)对结合型胆汁酸诱导的小鼠原代肝细胞IL-6表达水平的影响及机制。

方法

体外培养小鼠原代肝细胞，并用25 μmol/L的结合型胆汁酸处理，CCK-8法检测青蒿琥酯对小鼠原代肝细胞的毒性影响，RT-PCR法检测细胞因子IL-6的mRNA表达水平，蛋白印迹法检测肝细胞IL-6，p65

p-p65的水平。

结果

25 μmol/L结合型胆汁酸处理小鼠原代肝细胞能够诱导小鼠原代肝细胞IL-6的mRNA和蛋白质表达；青蒿琥酯减轻了TCA诱导的p65磷酸化和IL-6的蛋白水平；进一步研究发现青蒿琥酯是通过抑制p-p65(phosphorylated-p65)信号通路减轻小鼠原代肝细胞IL-6的表达。

结论

青蒿琥酯通过抑制p-p65信号通路减轻结合型胆汁酸诱导的原代小鼠肝细胞IL-6的产生。

Abstract

Objective

To investigate the effect and underlying mechanism of artesunate (AS) on the expression of interleukin-6 (IL-6) in mouse primary hepatocytes induced by conjugate bile acids.

Methods

Mouse hepatocytes were isolated and primarily cultured

and then treated with 25 μmol/L conjugated bile acids. CCK-8 assay was used to determine the toxic effects of AS on the liver cells. The mRNA level of IL-6 in the cells was measured by RT-qPCR

and the protein levels of IL-6

p-65 and phosphorylated-p65 (p-p65) were determined by Western blotting.

Results

Conjugated bile acids of 25 μmol/L significantly promoted the expression of IL-6 at mRNA and protein levels in mouse primary hepatocytes

while

AS reduced the taurocholic acid (TCA)-induced phosphorylation of p65 and IL-6 level. Further studies revealed that AS suppressed the IL-6 expression through inhibiting p-p65 signaling pathway in mouse primary hepatocytes.

Conclusion

AS reduces IL-6 expression in mouse primary hepatocytes induced by conjugate bile acids

via

inhibiting the p-p65 signaling pathway.

关键词

Keywords

references

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