Efficacy of bio-targeted drug-loaded nanoparticles on diagnosis and treatment for breast cancer based on hypoxic environment

Lu WANG; Fujie JIANG; Haiyan YANG; Disen WANG; Yaotai WANG; Yu TANG; Qi WANG; Jianzhong ZOU

doi:10.16016/j.1000-5404.202012046

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Efficacy of bio-targeted drug-loaded nanoparticles on diagnosis and treatment for breast cancer based on hypoxic environment

更新时间：2026-01-09

- Efficacy of bio-targeted drug-loaded nanoparticles on diagnosis and treatment for breast cancer based on hypoxic environment
- Journal of Third Military Medical University Vol. 43, Issue 10, Pages: 946-954(2021)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.16016/j.1000-5404.202012046
  CLC：
- Received：05 December 2020，
  
  Published：30 May 2021
- 稿件说明：
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Lu WANG, Fujie JIANG, Haiyan YANG, et al. Efficacy of bio-targeted drug-loaded nanoparticles on diagnosis and treatment for breast cancer based on hypoxic environment[J]. Journal of Third Military Medical University, 2021, 43(10): 946-954.
DOI：

Lu WANG, Fujie JIANG, Haiyan YANG, et al. Efficacy of bio-targeted drug-loaded nanoparticles on diagnosis and treatment for breast cancer based on hypoxic environment[J]. Journal of Third Military Medical University, 2021, 43(10): 946-954. DOI： 10.16016/j.1000-5404.202012046.

摘要

Abstract

Objective

To investigate the efficacy of bio-targeted drug loaded nanoparticles for the diagnosis and treatment of breast cancer based on tumor hypoxia microenvironment.

Methods

Lipid nanoparticles loaded with perfluorohexane (PFH) and tirazamine (TPZ) were prepared by film hydration. Bifidobacteria were cultured and detected for basic characterizations. Then

the bacteria and nanoparticles were connected by electrostatic adsorption to prepare bio-targeted drug-loaded nanoparticles

and laser confocal microscopy and flow cytometry were used to detect the binding between them. Ultrasonic diagnostic imaging

MTT assay and flow cytometry were adopted to observe the

in vitro

imaging performance and killing effect on breast cancer cells after high-intensity focused ultrasound (HIFU) irradiation. Tissue homogenate was used to observe the targeting ability of bifidobacteria.

In vivo

fluorescence imaging was performed to monitor the targeting of nanoparticles. The 4T1 breast tumor-bearing mice were established and randomly divided into targeted group (

=3) and non-targeted group (

=3) to observe the ultrasound imaging performance of nanoparticles. The tumor-bearing mice were randomly divided into blank control group

PBS+HIFU group

nonoparticles+HIFU group

and nonoparticles+ bifidobacterium+HIFU group (

=3 for each group). The body weight and tumor volume were recorded during treatment process

HE staining was performed 14 d later to observe the morphological changes of the tumor mass.

Results

Bio-targeted drug loaded nanoparticles were successfully prepared. The nanoparticles had an average size of 218.93±6.56 nm and a zet

a potential of 55.00±4.97 mV

and the bifidobacterium had a potential of -28.10±7.52 mV. The connection rate of the nanoparticles and bacterium was (99.04±0.40)%. The nanoparticles showed better ultrasound imaging after HIFU irradiation. The results of MTT assay and flow cytometry showed that the nanoparticles exerted better killing effect on breast cancer cells under hypoxic condition than under normoxia condition (

0.05). The results of tissue homogenate demonstrated that bifidobacteria showed good target ability.

In vivo

fluorescence imaging displayed that the nanoparticles were enriched around the tumor.

In vivo

ultrasound imaging was achieved in the targeted group after HIFU irradiation. Both the records of tumor volume and results of HE staining indicated that the nonoparticles+bifidobacterium+HIFU group got the best tumor inhibitory effect (

0.05).

Conclusion

The bio-targeted drug loaded nanoparticles can effectively diagnose and treat breast cancer based on hypoxia microenvironment. Our study provides a new thought for the integration of tumor diagnosis and treatment.

关键词

Keywords

references

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