Down-regulation of calreticulin promotes apoptosis in hepatic stellate cells

Linyu DAI; Yu YAN; Luguang WANG; Yonghong XIAO

doi:10.16016/j.1000-5404.202012010

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Down-regulation of calreticulin promotes apoptosis in hepatic stellate cells

更新时间：2026-01-09

- Down-regulation of calreticulin promotes apoptosis in hepatic stellate cells
- Journal of Third Military Medical University Vol. 43, Issue 10, Pages: 908-914(2021)
- 作者机构：
- 作者简介：
- 基金信息：
  
  the Natural Science Foundation of Hebei Province(H2015209043)
- DOI：10.16016/j.1000-5404.202012010
  CLC：
- Received：01 December 2020，
  
  Published：30 May 2021
- 稿件说明：
移动端阅览
Linyu DAI, Yu YAN, Luguang WANG, et al. Down-regulation of calreticulin promotes apoptosis in hepatic stellate cells[J]. Journal of Third Military Medical University, 2021, 43(10): 908-914.
DOI：

Linyu DAI, Yu YAN, Luguang WANG, et al. Down-regulation of calreticulin promotes apoptosis in hepatic stellate cells[J]. Journal of Third Military Medical University, 2021, 43(10): 908-914. DOI： 10.16016/j.1000-5404.202012010.

摘要

Abstract

Objective

To investigate the effect of calreticulin (CALR) down-regulation on apoptosis and endoplasmic reticulum stress (ERS) pathway in hepatic stellate cells (HSCs).

Methods

Small interfering RNA (siRNA) of CALR was transfected into HSC

via

liposome

and pro-fibrosis factor

namely transforming growth factor-β

(TGF-β

) was subsequently added in 24 h later. Another 24 h later

the morphology of HSCs was observed under an inverted microscope

cell apoptosis was detected by TUNEL assay

intracellular Ca

level was tested with a laser confocal microscope

and the expression levels of CALR

GRP78

Caspase-12 and Caspase-3 were determined using Western blotting.

Results

As compared with the control and negative-siRNA group

the apoptotic rate was significantly higher (

0.05)

and the concentration of Ca

and protein levels of GRP78

Caspase-12 and Caspase-3 were all increased (

0.05) in the CALR-siRNA group. However

TGF-β

treatment for 24 h decreased the number of apoptotic cells

concentration of Ca

and expression levels of above proteins when compared with the CALR-siRNA group.

Conclusion

Down-regulation of CALR promotes apoptosis of HSCs

which may be related to the imbalance of intracellular Ca

homeostasis and activation of the ERS pathway.

关键词

Keywords

references

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