Effect of oncostatin M on proliferation and secretion of extracellular matrix in human dermal fibroblasts in high glucose condition

Lulu LIU; Xueming FU; Biyu XUE; Bin XUE

doi:10.16016/j.1000-5404.202011253

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Effect of oncostatin M on proliferation and secretion of extracellular matrix in human dermal fibroblasts in high glucose condition

更新时间：2026-01-09

- Effect of oncostatin M on proliferation and secretion of extracellular matrix in human dermal fibroblasts in high glucose condition
- Journal of Third Military Medical University Vol. 43, Issue 10, Pages: 955-963(2021)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.16016/j.1000-5404.202011253
  CLC：
- Received：27 November 2020，
  
  Published：30 May 2021
- 稿件说明：
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Lulu LIU, Xueming FU, Biyu XUE, et al. Effect of oncostatin M on proliferation and secretion of extracellular matrix in human dermal fibroblasts in high glucose condition[J]. Journal of Third Military Medical University, 2021, 43(10): 955-963.
DOI：

Lulu LIU, Xueming FU, Biyu XUE, et al. Effect of oncostatin M on proliferation and secretion of extracellular matrix in human dermal fibroblasts in high glucose condition[J]. Journal of Third Military Medical University, 2021, 43(10): 955-963. DOI： 10.16016/j.1000-5404.202011253.

摘要

Abstract

Objective

To investigate the effects of oncostatin M (OSM) on the proliferation and extracellular matrix secretion of human dermal fibroblasts (HDFs) in high glucose environment and its possible mechanism.

Methods

① Establishment of hyperglycemia model: HDFs were treated with 5

20 and 25 mmol/L glucose

respectively. MTT assay was used to detect the proliferation activity of cells cultured in high glucose for 24

36 or 48 h. Western blotting was used to detect the protein levels of ERK

p-ERK and CyclinD1 in the HDFs treated with 5 or 20 mmol/L glucose for 24

36 and 48 h. ② Optimal concentration of OSM on hyperglycemia model: the cells were treated with 20 mmol/L glucose (control group)

and 20 mmol/L glucose+25

100

200 ng/mL OSM

respectively. MTT assay was used to detect the proliferation of above cells cultured for 24

36 and 48 h. The protein expression of CollagenⅠ

CollagenⅢ and fibronectin was detected by Western blotting. ③ Mechanism of OSM on hyperglycemia model: the cells were divided into control group

high glucose group

high glucose+OSM group

and high glucose+OSM+ inhibitor PD98059 group. Cell cycle was determined by flow cytometry. The protein levels of ERK

p-ERK

CyclinD1

CollagenⅠ

CollagenⅢ

fibronectin and other related factors were detected by Western blotting. q-PCR was used to measure the levels of ERK1/2

CyclinD1

CollagenⅠ

CollagenⅢ and fibronectin in each group.

Results

Treatment of 20 mmol/L glucose resulted in significantly decreased proliferation activity (

0.001)

increased proportion of G

cells (

0.01) and decreased proportion of S-phase cells (

0.01)

and reduced expression of ERK

p-ERK

CyclinD1

CollagenⅠ

CollagenⅢ and fibronectin (

0.01

0.05) when compared with the control cells. While treatment of 100 ng/mL OSM for 48 h enhanced the proliferation activity (

0.001)

decreased the proportion of G

cells (

0.05) but elevated the proportion of S-phase cells (

0.05)

and raised protein levels of ERK

p-ERK

CyclinD1

CollagenⅠ

CollagenⅢ and fibronectin cells (all

0.01). q-PCR indicated similar results as Western blotting

and statistical differences were seen in the results of mRNA levels (all

0.05). When ERK pathway specific small molecule inhibitor PD98059 blocked ERK signaling pathway

the above effects were significantly reduced (

0.05).

Conclusion

OSM may reverse the inhibitory effect of high glucose on the proliferation and extracellular matrix secretion through ERK signaling pathway in HDFs.

关键词

Keywords

references

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