Effects of ghrelin on pyroptosis pathway and inflammatory factors in mice with septic lung injury

Xiu YUAN; Shaojun LI; Chunxiao FU; Liang ZHOU; Zhiping ZOU; Liping TAN

doi:10.16016/j.1000-5404.202011142

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Effects of ghrelin on pyroptosis pathway and inflammatory factors in mice with septic lung injury

更新时间：2026-01-09

- Effects of ghrelin on pyroptosis pathway and inflammatory factors in mice with septic lung injury
- Journal of Third Military Medical University Vol. 43, Issue 10, Pages: 930-937(2021)
- 作者机构：
- 作者简介：
- 基金信息：
  
  the Natural Science Foundation of Chongqing(CSTC2020jcyj-msxmX0642)
- DOI：10.16016/j.1000-5404.202011142
  CLC：
- Received：16 November 2020，
  
  Published：30 May 2021
- 稿件说明：
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Xiu YUAN, Shaojun LI, Chunxiao FU, et al. Effects of ghrelin on pyroptosis pathway and inflammatory factors in mice with septic lung injury[J]. Journal of Third Military Medical University, 2021, 43(10): 930-937.
DOI：

Xiu YUAN, Shaojun LI, Chunxiao FU, et al. Effects of ghrelin on pyroptosis pathway and inflammatory factors in mice with septic lung injury[J]. Journal of Third Military Medical University, 2021, 43(10): 930-937. DOI： 10.16016/j.1000-5404.202011142.

摘要

Abstract

Objective

To analyze the effects of ghrelin on the pyroptosis pathway and the expression of inflammatory factors in septic lung injury

and explore its protective mechanism.

Methods

A total of 180 healthy male C57 BL/6 mice were divided into sham operation group (sham

=24)

cecal ligation and puncture group (CLP

=78) and ghrelin group (

=78). The ghrelin group was intraperitoneally given ghrelin 12 nmol/kg at 3 h and 20 h after CLP surgery

while the other groups were given the same amount of normal saline. The 120-hour survival rate of each group was observed

and the bronchoalveolar lavage fluid (BALF) and lung tissues of mice in 6

12 and 24 h after CLP group

ghrelin group and in 24 h for sham groups were collected respectively

and the total protein concentration and total cell number in BALF were detected. The expression of ghrelin as well as inflammatory cytokines such as IL-10

IL-6

TNF-α

IL-1β and IL-18 was tested by ELISA. Moreover

the expression of gasdermin D (GSDMD) was determined by immunofluorescence staining

and the levels of pyroptosis signal pathway proteins including NOD-like receptor protein (NLRP3)

ASC

Caspase-1 and GSDMD were detected by qPCR and Western blotting.

Results

As compared with sham group

the mortality

pathological changes of lung tissue and levels of inflammatory factors (IL-10

IL-6

TNF-α

IL-1β and IL-18) in CLP group were significantly increased (

0.05); the level of ghrelin in the lung tissue was dropped

while those of NLRP3

ASC

Caspase-1 and GSDMD rose in the CLP group (

0.05). After administration of exogenous ghrelin

the death rate of septic lung injury mice was declined

the pathological damage of lung tissue was alleviated

and the expression levels of inflammatory cytokines and NLRP3

ASC

Caspase-1 and GSDMD were all decreased (

0.05).

Conclusion

Ghrelin improves the survival rate of septic lung injury mice and reduces the pathological changes of lung tissue

which may play a protective role by inhibiting the pathway of pyroptosis and alleviating inflammation.

关键词

Keywords

references

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