Effects of circular RNA circVCAN on proliferation, invasion and autophagy of placental trophoblast cells

Xiaolan YUAN; Li LIU; Lei ZHANG; Daiyu TANG; Tong YANG; Xiaojing DONG

doi:10.16016/j.1000-5404.202010070

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Effects of circular RNA circVCAN on proliferation, invasion and autophagy of placental trophoblast cells

更新时间：2026-01-09

- Effects of circular RNA circVCAN on proliferation, invasion and autophagy of placental trophoblast cells
- Journal of Third Military Medical University Vol. 43, Issue 6, Pages: 489-495(2021)
- 作者机构：
- 作者简介：
- 基金信息：
  
  the Natural Science Foundation of Chongqing(cstc2019jcyj-msxmX0318);the Innovation and Intelligence Project of International Cooperation Joint Laboratory of Reproduction and Development of Ministry of Education and Higher Education
- DOI：10.16016/j.1000-5404.202010070
  CLC：
- Received：14 October 2020，
  
  Published：30 March 2021
- 稿件说明：
移动端阅览
Xiaolan YUAN, Li LIU, Lei ZHANG, et al. Effects of circular RNA circVCAN on proliferation, invasion and autophagy of placental trophoblast cells[J]. Journal of Third Military Medical University, 2021, 43(6): 489-495.
DOI：

Xiaolan YUAN, Li LIU, Lei ZHANG, et al. Effects of circular RNA circVCAN on proliferation, invasion and autophagy of placental trophoblast cells[J]. Journal of Third Military Medical University, 2021, 43(6): 489-495. DOI： 10.16016/j.1000-5404.202010070.

摘要

Abstract

Objective

To investigate the expression

function and potential mechanism of circular RNA circVCAN(also known as hsa-circ-0073239)in fetal growth restriction (FGR) placenta.

Methods

The placenta tissue samples were collected from 24 pregnant women (12 with FGR and 12 normal pregnancy) admitted in our hospital from January to December 2018. Real-time quantitative PCR (RT-qPCR) was performed to detect the expression of circVCAN in these placenta samples. Human placental trophoblast cell line HTR8/SVneo was cultured and then transfected with circVCAN interference and overexpression plasmid

respectively. EdU assay

flow cytometer and Transwell assay were carried out respectively to verify the variations in the proliferation

apoptosis and invasion abilities of the cells. And the expression of autophagy related proteins LC3Ⅱ/LC3Ⅰ

ATG5 and ATG7 was measured by Western blotting.

Results

The expression levels of circVCAN

ATG5

ATG7 and LC3Ⅱ/LC3Ⅰwere significantly higher in FGR placenta tissue than in the normal group (

0.01). The termination of pregnancy was earlier in FGR patients than in normal group (

0.05)

with newborns from the FGR group having lower birth body weight (

0.01) and placenta weight (

0.05) as well as shorter birth body length (

0.01) when compared with those from the normal group. Silencing of circVCAN resulted in enhanced proliferation and invasion abilities in HTR8/SVneo cells (

0.01)

while circVCAN overexpression led to decreased proliferation and invasion abilities in the cells (

0.01)

but no statistical difference was seen in the apoptotic rate (

0.05). The expression levels of autophagy related proteins LC3Ⅱ/LC3Ⅰ

ATG5 and ATG7 were significantly lower in circVCAN interfered cells (

0.05)

while higher in circVCAN overexpressed cells (

0.05).

Conclusion

CircVCAN is highly expressed in FGR placenta tissue

though it showing no effect on apoptosis but inhibiting the proliferation and invasion of HTR8/SVneo trophoblast cells

and may enhance autophagy by promoting the expression of ATG5 and ATG7.

关键词

Keywords

references

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