Respiratory syncytial virus promotes apoptosis and expression of inflammatory factors in bronchial epithelial cells by regulating miR-409-3p/SIRT1 pathway

Juanjuan MEI; Man TIAN; Shasha YAN; Cong GAN; Wenxin ZHANG

doi:10.16016/j.1000-5404.202010016

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Respiratory syncytial virus promotes apoptosis and expression of inflammatory factors in bronchial epithelial cells by regulating miR-409-3p/SIRT1 pathway

更新时间：2026-01-09

- Respiratory syncytial virus promotes apoptosis and expression of inflammatory factors in bronchial epithelial cells by regulating miR-409-3p/SIRT1 pathway
- Journal of Third Military Medical University Vol. 43, Issue 6, Pages: 503-511(2021)
- 作者机构：
- 作者简介：
- 基金信息：
  
  the Project of Medical Science and Technology Development of Nanjing(ZKX17036)
- DOI：10.16016/j.1000-5404.202010016
  CLC：
- Received：09 October 2020，
  
  Published：30 March 2021
- 稿件说明：
移动端阅览
Juanjuan MEI, Man TIAN, Shasha YAN, et al. Respiratory syncytial virus promotes apoptosis and expression of inflammatory factors in bronchial epithelial cells by regulating miR-409-3p/SIRT1 pathway[J]. Journal of Third Military Medical University, 2021, 43(6): 503-511.
DOI：

Juanjuan MEI, Man TIAN, Shasha YAN, et al. Respiratory syncytial virus promotes apoptosis and expression of inflammatory factors in bronchial epithelial cells by regulating miR-409-3p/SIRT1 pathway[J]. Journal of Third Military Medical University, 2021, 43(6): 503-511. DOI： 10.16016/j.1000-5404.202010016.

摘要

Abstract

Objective

To explore the effect of respiratory syncytial virus (RSV) on the apoptosis and the expression of inflammatory factors in human bronchial epithelial cells (HBECs)

and investigate its possible mechanisms.

Methods

In vitro

cultured HBECs were transfected with miR-409-3p inhibitor

overexpression vector of silent information regulator 1 (SIRT1) along

or co-transfected with miR-409-3p inhibitor and SIRT1 small interfering RNA (siRNA)

respect

ively. After RSV infection

RT-qPCR was used to detect the expression levels of miR-409-3p and SIRT1 in the transfected cells. Cell counting kit-8 (CCK-8) assay and flow cytometry were performed to determine cell proliferation as well as cell apoptosis. Western blotting was also adopted to verify the expression of Ki-67

p21

Bcl-2 and Bax. In addition

corresponding reagent testing kits were used to detect the contents of IL-6 and TNF-α in culture supernatant. Finally

the regulatory relationship between SIRT1 and miR-409-3p was verified by the dual luciferase reporter assay.

Results

RSV infection promoted the expression of miR-409-3p (

0.05)

inhibited that of SIRT1 (

0.05)

decreased cell proliferation and protein expression of Ki-67 and Bcl2 (

0.05)

and promoted cell apoptosis and protein expression of p21 and Bax and contents of IL-6 and TNF-α (

0.05) in HBECs cells. Silencing miR-409-3p or overexpressing SIRT1 resulted in the enhanced the proliferation activity and the protein expression of Ki-67 and Bcl2 in RSV-infected HBECs cells (

0.05)

but decreased apoptotic rate

protein expression of p21 and Bax

and contents of IL-6 and TNF-α (

0.05). miR-409-3p showed a negatively targeted regulation to SIRT1 expression. Silencing SIRT1 reversed the effects of silenced miR-409-3p on the proliferation and apoptosis of RSV-infected HBECs as well as on the expression of inflammatory factors IL-6 and TNF-α (

0.05).

Conclusion

RSV promotes the apoptosis and the expression of inflammatory factors in bronchial epithelial cells by regulating miR-409-3p/SIRT1 pathway.

关键词

Keywords

references

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