Correlation of NPRL2 with cinicopathological features of bladder cancer and its effect on cell apoptosis

Xi ZHU; Shengjun LUO; Li JIANG; Wei TANG

doi:10.16016/j.1000-5404.202009233

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Correlation of NPRL2 with cinicopathological features of bladder cancer and its effect on cell apoptosis

更新时间：2026-01-09

- Correlation of NPRL2 with cinicopathological features of bladder cancer and its effect on cell apoptosis
- Journal of Third Military Medical University Vol. 43, Issue 5, Pages: 432-437(2021)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.16016/j.1000-5404.202009233
  CLC：
- Received：27 September 2020，
  
  Published：15 March 2021
- 稿件说明：
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Xi ZHU, Shengjun LUO, Li JIANG, et al. Correlation of NPRL2 with cinicopathological features of bladder cancer and its effect on cell apoptosis[J]. Journal of Third Military Medical University, 2021, 43(5): 432-437.
DOI：

Xi ZHU, Shengjun LUO, Li JIANG, et al. Correlation of NPRL2 with cinicopathological features of bladder cancer and its effect on cell apoptosis[J]. Journal of Third Military Medical University, 2021, 43(5): 432-437. DOI： 10.16016/j.1000-5404.202009233.

摘要

Abstract

Objective

To investigate the correlation between expression of NPRL2 and clinicopathological features of bladder cancer and the effect of NPRL2 silencing on the apoptosis of bladder cancer cell line Biu87/RT-4.

Methods

Cancer tissue samples were collected from 131 patients with bladder cancer admitted into our department from January 2014 to January 2019. The expression of NPRL2 in bladder cancer tissues was detected by immunohistochemistry

and the correlation between NPRL2 and the clinicopathological features of bladder cancer was analyzed. The expression of NPRL2 in CP-H068

Biu87 and RT-4 cell lines was detected

and the cell proliferation after NPRL2 silencing was detected by CCK-8 assay. Flow cytometry was used to detect cell apoptosis

and Western blotting to detect the expression of apoptosis-related proteins.

Results

NPRL2 was highly expressed in bladder cancer samples

accounting for 53.44%

and the expression was significantly correlated with the clinical stage and grade of bladder cancer (

0.05). Its expression was also obviously higher in the bladder cancer Biu87 and RT-4 cells than the normal bladder epithelial CP-H068 cells (

0.05). NPRL2 silencing resulted in decreased proliferation ability

elevated apoptotic rate

and increased protein levels of JNK

Bax and caspase-9

while decreased level of Bcl-2 in the Biu87 and RT-4 cells (

0.05).

Conclusion

The expression of NPRL2 is related to the clinical stage and grade of bladder cancer. Silencing NPRL2 in bladder cancer cells Biu87/RT-4 can effectively inhibit the proliferation and promote apoptosis of bladder cancer cells.

关键词

Keywords

references

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