Analysis of semen parameters and sex hormones under low-dose PAEs exposure in Chongqing college students and comparison with reference dose

Yimeng WANG; Qing CHEN; Kun LIU; Huan YANG; Niya ZHOU; Lei SUN; Peng ZOU; Xi LING; Lin AO; Jinyi LIU; Jia CAO

doi:10.16016/j.1000-5404.202009207

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Analysis of semen parameters and sex hormones under low-dose PAEs exposure in Chongqing college students and comparison with reference dose

更新时间：2026-01-09

- Analysis of semen parameters and sex hormones under low-dose PAEs exposure in Chongqing college students and comparison with reference dose
- Journal of Third Military Medical University Vol. 43, Issue 1, Pages: 31-37(2021)
- 作者机构：
- 作者简介：
- 基金信息：
  
  the Key Program of National Natural Science Foundation of China(81630087);the National Key Research and Development Program of China(2017YFC1002001)
- DOI：10.16016/j.1000-5404.202009207
  CLC：
- Received：24 September 2020，
  
  Published：15 January 2021
- 稿件说明：
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Yimeng WANG, Qing CHEN, Kun LIU, et al. Analysis of semen parameters and sex hormones under low-dose PAEs exposure in Chongqing college students and comparison with reference dose[J]. Journal of Third Military Medical University, 2021, 43(1): 31-37.
DOI：

Yimeng WANG, Qing CHEN, Kun LIU, et al. Analysis of semen parameters and sex hormones under low-dose PAEs exposure in Chongqing college students and comparison with reference dose[J]. Journal of Third Military Medical University, 2021, 43(1): 31-37. DOI： 10.16016/j.1000-5404.202009207.

摘要

Abstract

Objective

To analyze the associations of diethyl phthalate (DEP)

di-n-butyl phthalate (DnBP)

di(2-ethyl)hexyl phthalate (DEHP) and butyl benzyl phthalate (BBzP) [which have reference doses (RfD) in the plasticizer

phthalates (PAEs)] with semen parameters and sex hormone levels in the young male adults

and evaluate the safety of RfD.

Methods

A total of 796 subjects were sampled from our previously established cohort of the male reproductive health in Chongqing college students (MARHCS) study. The contents of PAEs metabolites were detected in the urine samples of the subjects to estimate the daily intake of PAEs. Optimal scaling regression analysis was used to test their associations of the contents with semen parameters and sex hormone levels. Joinpoint regression model was applied to explore whether there were inflection points in the above associations

and the results were compared with RfD.

Results

Except for the metabolite of BBzP (MBzP) below the detection limit in the most individua

the median daily intake of the other 3 PAEs was 0.1

1.0 and 4.3 μg/(kg·d) for DEP

DnBP and DEHP

which were all significantly lower than the corresponding RfD [800

100 and 20 μg/(kg·d)

]

and health guidance values [30

10 and 50 μg/(kg·d)

]

. The metabolite of DEP (MEP) was correlated with the progressive motility

normal sperm morphology

and testosterone level (

0.01); DnBP metabolite (MnBP) was correlated with normal sperm morphology (

0.05). Among the 4 metabolites of DEHP

MEHHP and MECPP were related to estrogen level (

0.05)

and MEOHP and MEHP to estrogen and progesterone levels (

0.01). But

inflection points were only observed in the correlations between DEHP and some parameters [threshold doses are 2.9~3.6 and 5.0~5.8 μg/(kg·d)

]

Conclusion

There are 12 kinds of associations of PAEs exposure with semen parameters and sex hormone levels in college students in Chongqing. PAEs

though below RfD

can still causes male reproductive damage.

关键词

Keywords

references

王文枝国伟孙利食品包装材料中DEHP的危害及其在食品中的暴露评估食品科技 2008 33 4 166 168 10.3969/j.issn.1005-9989.2008.04.049 王文枝, 国伟, 孙利, 等.食品包装材料中DEHP的危害及其在食品中的暴露评估[J].食品科技, 2008, 33(4): 166-168.

WANG W Z GUO W SUN L The hazard and exposure assessment of DEHP in food contact materials Food Sci Technol 2008 33 4 166 168 10.3969/j.issn.1005-9989.2008.04.049 WANG W Z, GUO W, SUN L, et al . The hazard and exposure assessment of DEHP in food contact materials[J ] . Food Sci Technol, 2008, 33(4): 166-168.

CHIANG H C KUO Y T SHEN C C Mono(2-ethylhexyl)phthalate accumulation disturbs energy metabolism of fat cells Arch Toxicol 2016 90 3 589 601 10.1007/s00204-014-1446-9 CHIANG H C, KUO Y T, SHEN C C, et al . Mono(2-ethylhexyl)phthalate accumulation disturbs energy metabolism of fat cells[J ] . Arch Toxicol, 2016, 90(3): 589-601. DOI:10.1007/s00204-014-1446-9.

KARABULUT G BARLAS N Genotoxic, histologic, immunohistochemical, morphometric and hormonal effects of di-(2-ethylhexyl)-phthalate (DEHP) on reproductive systems in pre-pubertal male rats Toxicol Res 2018 7 5 859 873 10.1039/c8tx00045j KARABULUT G, BARLAS N. Genotoxic, histologic, immunohistochemical, morphometric and hormonal effects of di-(2-ethylhexyl)-phthalate (DEHP) on reproductive systems in pre-pubertal male rats[J]. Toxicol Res, 2018, 7(5): 859-873. DOI:10.1039/c8tx00045j.

ZHANG G YANG W JIANG F PERK regulates Nrf2/ARE antioxidant pathway against dibutyl phthalate-induced mitochondrial damage and apoptosis dependent of reactive oxygen species in mouse spermatocyte-derived cells Toxicol Lett 2019 308 24 33 10.1016/j.toxlet.2019.03.007 ZHANG G, YANG W, JIANG F, et al . PERK regulates Nrf2/ARE antioxidant pathway against dibutyl phthalate-induced mitochondrial damage and apoptosis dependent of reactive oxygen species in mouse spermatocyte-derived cells[J ] . Toxicol Lett, 2019, 308: 24-33. DOI:10.1016/j.toxlet.2019.03.007 .

WANG S Y WANG Y XIE F Q Analysis of PAEs in semen of infertile men Int J Occup Environ Health 2015 21 1 40 48 10.1179/2049396714y.0000000088 WANG S Y, WANG Y, XIE F Q, et al . Analysis of PAEs in semen of infertile men[J ] . Int J Occup Environ Health, 2015, 21(1): 40-48. DOI:10.1179/2049396714y.0000000088.

BLOOM M S WHITCOMB B W CHEN Z Associations between urinary phthalate concentrations and semen quality parameters in a general population Hum Reprod 2015 30 11 2645 2657 10.1093/humrep/dev219 BLOOM M S, WHITCOMB B W, CHEN Z, et al . Associations between urinary phthalate concentrations and semen quality parameters in a general population[J ] . Hum Reprod, 2015, 30(11): 2645-2657. DOI:10.1093/humrep/dev219.

STRASSLE P D SMIT L HOPPIN J A Endotoxin enhances respiratory effects of phthalates in adults: Results from NHANES 2005-6 Environ Res 2018 162 280 286 10.1016/j.envres.2018.01.017 STRASSLE P D, SMIT L, HOPPIN J A. Endotoxin enhances respiratory effects of phthalates in adults: Results from NHANES 2005-6[J]. Environ Res, 2018, 162: 280-286. DOI:10.1016/j.envres.2018.01.017.

JOHNS L E COOPER G S GALIZIA A Exposure assessment issues in epidemiology studies of phthalates Environ Int 2015 85 27 39 10.1016/j.envint.2015.08.005 JOHNS L E, COOPER G S, GALIZIA A, et al . Exposure assessment issues in epidemiology studies of phthalates[J ] . Environ Int, 2015, 85: 27-39. DOI:10.1016/j.envint.2015.08.005.

SILANO V BARAT B J BOLOGNESI C Update of the risk assessment of di-butylphthalate (DBP), butyl-benzyl-phthalate (BBP), bis(2-ethylhexyl)phthalate (DEHP), di-isononylphthalate (DINP) and di-isodecylphthalate (DIDP) for use in food contact materials EFSA J 2019 17 12 e5838 10.2903/j.efsa.2019.5838 SILANO V, BARAT B J, BOLOGNESI C, et al . Update of the risk assessment of di-butylphthalate (DBP), butyl-benzyl-phthalate (BBP), bis(2-ethylhexyl)phthalate (DEHP), di-isononylphthalate (DINP) and di-isodecylphthalate (DIDP) for use in food contact materials[J ] . EFSA J, 2019, 17(12): e5838. DOI: 10.2903/j.efsa.2019.5838.

王彝白纳蒋定国杨大进中国居民邻苯二甲酸二丁酯膳食摄入水平及其风险评估中国食品卫生杂志 2016 28 6 800 804 https://www.cnki.com.cn/Article/CJFDTOTAL-ZSPZ201606025.htm 王彝白纳, 蒋定国, 杨大进, 等.中国居民邻苯二甲酸二丁酯膳食摄入水平及其风险评估[J].中国食品卫生杂志, 2016, 28(6): 800-804.

WANG Y B N JIANG D G YANG D J Dietary intake and risk assessment of dibutyl phthalate in Chinese population Chin J Food Hyg 2016 28 6 800 804 https://www.cnki.com.cn/Article/CJFDTOTAL-ZSPZ201606025.htm WANG Y B N, JIANG D G, YANG D J, et al . Dietary intake and risk assessment of dibutyl phthalate in Chinese population[J ] . Chin J Food Hyg, 2016, 28(6): 800-804.

隋海霞蒋定国吴平谷中国居民邻苯二甲酸二(2-乙基己基)酯膳食摄入水平及其风险评估中华预防医学杂志 2015 49 3 218 222 10.3760/cma.j.issn.0253-9624.2015.03.006 隋海霞, 蒋定国, 吴平谷, 等.中国居民邻苯二甲酸二(2-乙基己基)酯膳食摄入水平及其风险评估[J].中华预防医学杂志, 2015, 49(3): 218-222.

SUI H X JIANG D G WU P G Dietary intake and risk assessment of diethylhexyl phthalate in Chinese populations Chin J Prev Med 2015 49 3 218 222 10.3760/cma.j.issn.0253-9624.2015.03.006 SUI H X, JIANG D G, WU P G, et al . Dietary intake and risk assessment of diethylhexyl phthalate in Chinese populations[J ] . Chin J Prev Med, 2015, 49(3): 218-222.

YANG H CHEN Q ZHOU N Lifestyles associated with human semen quality: results from MARHCS cohort study in Chongqing, China Medicine (Madr) 2015 94 28 e1166 10.1097/md.0000000000001166 YANG H, CHEN Q, ZHOU N, et al . Lifestyles associated with human semen quality: results from MARHCS cohort study in Chongqing, China[J ] . Medicine (Madr), 2015, 94(28): e1166. DOI:10.1097/md.0000000000001166.

CHEN Q YANG H ZHOU N Phthalate exposure, even below US EPA reference doses, was associated with semen quality and reproductive hormones: Prospective MARHCS study in general population Environ Int 2017 104 58 68 10.1016/j.envint.2017.04.005 CHEN Q, YANG H, ZHOU N, et al . Phthalate exposure, even below US EPA reference doses, was associated with semen quality and reproductive hormones: Prospective MARHCS study in general population[J ] . Environ Int, 2017, 104: 58-68. DOI:10.1016/j.envint.2017.04.005.

WITTASSEK M KOCH H M ANGERER J Assessing exposure to phthalates—The human biomonitoring approach Mol Nutr Food Res 2011 55 1 7 31 10.1002/mnfr.201000121 WITTASSEK M, KOCH H M, ANGERER J, et al . Assessing exposure to phthalates—The human biomonitoring approach[J ] . Mol Nutr Food Res, 2011, 55(1): 7-31. DOI:10.1002/mnfr.201000121.

LIU K HOU G WANG X Adverse effects of circadian desynchrony on the male reproductive system: an epidemiological and experimental study Hum Reprod 2020 35 7 1515 1528 10.1093/humrep/deaa101 LIU K, HOU G, WANG X, et al . Adverse effects of circadian desynchrony on the male reproductive system: an epidemiological and experimental study[J ] . Hum Reprod, 2020, 35(7): 1515-1528. DOI:10.1093/humrep/deaa101.

WANG X CHEN Q ZOU P Sleep duration is associated with sperm chromatin integrity among young men in Chongqing, China J Sleep Res 2018 27 4 e12615 10.1111/jsr.12615 WANG X, CHEN Q, ZOU P, et al . Sleep duration is associated with sperm chromatin integrity among young men in Chongqing, China[J ] . J Sleep Res, 2018, 27(4): e12615.DOI:10.1111/jsr.12615.

BENJAMINI Y HOCHBERG Y Controlling the false discovery rate: a practical and powerful approach to multiple testing J R Stat Soc Series B Stat Methodol 1995 57 1 289 300 10.1111/j.2517-6161.1995.tb02031.x BENJAMINI Y, HOCHBERG Y. Controlling the false discovery rate: a practical and powerful approach to multiple testing[J]. J R Stat Soc Series B Stat Methodol, 1995, 57(1): 289-300. DOI:10.1111/j.2517-6161.1995.tb02031.x.

AL-SALEH I COSKUN S AL-DOUSH I The relationships between urinary phthalate metabolites, reproductive hormones and semen parameters in men attending in vitro fertilization clinic Sci Total Environ 2019 658 982 995 10.1016/j.scitotenv.2018.12.261 AL-SALEH I, COSKUN S, AL-DOUSH I, et al . The relationships between urinary phthalate metabolites, reproductive hormones and semen parameters in men attending in vitro fertilization clinic[J ] . Sci Total Environ, 2019, 658: 982-995. DOI:10.1016/j.scitotenv.2018.12.261.

THURSTON S W MENDIOLA J BELLAMY A R Phthalate exposure and semen quality in fertile US men Andrology 2016 4 4 632 638 10.1111/andr.12124 THURSTON S W, MENDIOLA J, BELLAMY A R, et al . Phthalate exposure and semen quality in fertile US men[J ] . Andrology, 2016, 4(4): 632-638. DOI:10.1111/andr.12124.

WIRTH J J ROSSANO M G POTTER R A pilot study associating urinary concentrations of phthalate metabolites and semen quality Syst Biol Reproductive Med 2008 54 3 143 154 10.1080/19396360802055921 WIRTH J J, ROSSANO M G, POTTER R, et al . A pilot study associating urinary concentrations of phthalate metabolites and semen quality[J ] . Syst Biol Reproductive Med, 2008, 54(3): 143-154. DOI:10.1080/19396360802055921.

SPECHT I O TOFT G HOUGAARD K S Associations between serum phthalates and biomarkers of reproductive function in 589 adult men Environ Int 2014 66 146 156 10.1016/j.envint.2014.02.002 SPECHT I O, TOFT G, HOUGAARD K S, et al . Associations between serum phthalates and biomarkers of reproductive function in 589 adult men[J ] . Environ Int, 2014, 66: 146-156. DOI:10.1016/j.envint.2014.02.002.

杨敏魏丽刘长江 DEHP对大鼠睾丸氧化应激和血清睾酮水平影响中国公共卫生 2017 33 11 1599 1602 10.11847/zgggws2017-33-11-15 杨敏, 魏丽, 刘长江. DEHP对大鼠睾丸氧化应激和血清睾酮水平影响[J].中国公共卫生, 2017, 33(11): 1599-1602.

YANG M WEI L LIU C J DEHP-induced testicular oxidative stress and down-regulated serum testosterone in rats Chin J Public Heal 2017 33 11 1599 1602 10.11847/zgggws2017-33-11-15 YANG M, WEI L, LIU C J. DEHP-induced testicular oxidative stress and down-regulated serum testosterone in rats[J]. Chin J Public Heal, 2017, 33(11): 1599-1602.

TIAN M P LIU L P ZHANG J Positive association of low-level environmental phthalate exposure with sperm motility was mediated by DNA methylation: a pilot study Chemosphere 2019 220 459 467 10.1016/j.chemosphere.2018.12.155 TIAN M P, LIU L P, ZHANG J, et al . Positive association of low-level environmental phthalate exposure with sperm motility was mediated by DNA methylation: a pilot study[J ] . Chemosphere, 2019, 220: 459-467. DOI:10.1016/j.chemosphere.201 8.12.155.

KIJIMA K TOYOSAWA K YASUBA M Gene expression analysis of the rat testis after treatment with di(2-ethylhexyl) phthalate using cDNA microarray and real-time RT-PCR Toxicol Appl Pharmacol 2004 200 2 103 110 10.1016/j.taap.2004.03.015 KIJIMA K, TOYOSAWA K, YASUBA M, et al . Gene expression analysis of the rat testis after treatment with di(2-ethylhexyl) phthalate using cDNA microarray and real-time RT-PCR[J ] . Toxicol Appl Pharmacol, 2004, 200(2): 103-110. DOI:10.1016/j.taap.2004.03.015.

ZHANG Q HAO L C HONG Y Exposure evaluation of diisononyl phthalate in the adults of Drosophila melanogaster: Potential risks in fertility, lifespan, behavior, and modes of action Comp Biochem Phys C 2020 238 108847 10.1016/j.cbpc.2020.108847 ZHANG Q, HAO L C, HONG Y. Exposure evaluation of diisononyl phthalate in the adults of Drosophila melanogaster: Potential risks in fertility, lifespan, behavior, and modes of action[J]. Comp Biochem Phys C, 2020, 238: 108847. DOI:10.1016/j.cbpc.2020.108847.

AXELSSON J RYLANDER L RIGNELL-HYDBOM A Phthalate exposure and reproductive parameters in young men from the general Swedish population Environ Int 2015 85 54 60 10.1016/j.envint.2015.07.005 AXELSSON J, RYLANDER L, RIGNELL-HYDBOM A, et al . Phthalate exposure and reproductive parameters in young men from the general Swedish population[J ] . Environ Int, 2015, 85: 54-60. DOI:10.1016/j.envint.2015.07.005.

HUANG L P LEE C C HSU P C The association between semen quality in workers and the concentration of di(2-ethylhexyl) phthalate in polyvinyl chloride pellet plant air Fertil Steril 2011 96 1 90 94 10.1016/j.fertnstert.2011.04.093 HUANG L P, LEE C C, HSU P C, et al . The association between semen quality in workers and the concentration of di(2-ethylhexyl) phthalate in polyvinyl chloride pellet plant air[J ] . Fertil Steril, 2011, 96(1): 90-94. DOI:10.1016/j.fertnstert.2011.04.093.

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