Association between fine particulate matter bound polycyclic aromatic hydrocarbons and DNA damage of male sperm in Chongqing
- Journal of Third Military Medical University Vol. 43, Issue 1, Pages: 17-24(2021)
- 作者机构：
- 作者简介：
- 基金信息：the Key Program of National Natural Science Foundation of China(81630087);the National Key Research and Development Program of China(2017YFC1002001)
DOI：10.16016/j.1000-5404.202009206
CLC：Received：24 September 2020，
Published：15 January 2021
- 稿件说明：
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Furong WANG, Niya ZHOU, Qing CHEN, et al. Association between fine particulate matter bound polycyclic aromatic hydrocarbons and DNA damage of male sperm in Chongqing[J]. Journal of Third Military Medical University, 2021, 43(1): 17-24.
Furong WANG, Niya ZHOU, Qing CHEN, et al. Association between fine particulate matter bound polycyclic aromatic hydrocarbons and DNA damage of male sperm in Chongqing[J]. Journal of Third Military Medical University, 2021, 43(1): 17-24. DOI： 10.16016/j.1000-5404.202009206.
摘要
Abstract
Objective
2
To investigate the association between exposure to polycyclic aromat
ic hydrocarbons(PAHs) in atmospheric fine particulate matter(PM
2.5
) and sperm DNA damage in Chongqing.
Methods
2
The subjects were sampled from our previously established cohort of the male reproductive health in Chongqing college students(MARHCS) study. The concentrations of PAHs(collected during the sampling periods) with low fused aromatic rings(2~4 rings) and with high fused aromatic rings(5~6 rings) in the air PM
2.5
filter membranes were detected. Single cell gel electrophoresis(Comet assay) was used to evaluate DNA damage. Finally
the mixed-effects model was used to investigate the relationships between the exposure concentrations of PAHs components in PM
2.5
and severity of sperm DNA damage.
Results
2
In 2013
the low molecular weight(LMW) PAHs accounted for a large proportion in atmospheric fine particles
mainly phenanthrene
fluorene and pyrene. While
the high molecular weight(HMW) PAHs dominated in 2014
such as indeno[1
2
3-cd
]
pyrene
benzo[g
h
i
]
perylene and benzo[a
]
pyrene. Sperm DNA damage indicators
such as tail length
tail moment and Olive tail moment were more common in the cohort in the year of 2014 than in 2013(all
P
<
0.05). The results of the mixed model showed that the concentrations of fluoranthene
acenaphthene
pyrene and ∑LMW PAHs were negatively correlated with tail length and tail moment. The concentrations of benzo[k
]
fluoranthene
benzo[b
]
fluoranthene and dibenz[a
h
]
anthracene were positively correlated with tail length [
β
(95%
CI
): 108.987(43.754~203.820)
158.700(65.957~303.272)
185.708(67.350~387.775)
respectively; all corrected
P
<
0.05
]
and with tail moment [
β
(95%
CI
): 449.204(260.066~737.695)
683.108(374.515~1192.387)
683.108(374.515~1 192.387)
respectively; all corrected
P
<
0.05
]
.
Conclusion
2
The exposure of HMW PAHs in Chongqing atmospheric fine particles may lead to the enhancement of sperm DNA damage.
关键词
Keywords
references
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