Focused low-intensity pulsed ultrasound promotes chondrocyte mitophagy <italic style="font-style: italic">via</italic> up-regulating PGAM5 protein

Haixia YE; Lehua YU; Lang JIA

doi:10.16016/j.1000-5404.202009202

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Focused low-intensity pulsed ultrasound promotes chondrocyte mitophagy via up-regulating PGAM5 protein

更新时间：2026-01-09

- Focused low-intensity pulsed ultrasound promotes chondrocyte mitophagy via up-regulating PGAM5 protein
- Journal of Third Military Medical University Vol. 43, Issue 5, Pages: 403-410(2021)
- 作者机构：
- 作者简介：
- 基金信息：
  
  the National Natural Science Foundation for Young Scholar of China(81802234)
- DOI：10.16016/j.1000-5404.202009202
  CLC：
- Received：23 September 2020，
  
  Published：15 March 2021
- 稿件说明：
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Haixia YE, Lehua YU, Lang JIA. Focused low-intensity pulsed ultrasound promotes chondrocyte mitophagy via up-regulating PGAM5 protein[J]. Journal of Third Military Medical University, 2021, 43(5): 403-410.
DOI：

Haixia YE, Lehua YU, Lang JIA. Focused low-intensity pulsed ultrasound promotes chondrocyte mitophagy via up-regulating PGAM5 protein[J]. Journal of Third Military Medical University, 2021, 43(5): 403-410. DOI： 10.16016/j.1000-5404.202009202.

摘要

Abstract

Objective

To investigate the effect of focused low-intensity pulsed ultrasound (FLIPUS) on the activation of mitophagy in chondrocytes.

Methods

Chondrocytes were isolated from the knee articular cartilage of C57BL/6J suckling mice

and then primarily cultured. Then osteoarthritis-like chondrocyte injury was inflicted by IL-1β simulation in the chondrocytes and identified. The chondrocytes were divided into 3 groups

that is

control

IL-1β and IL-1β+FLIPUS group. The mRNA expression of Col2α1 and MMP13 was detected by quantitative real-time PCR

and the protein levels of Col2α1

MMP13

LC3B-Ⅱ

TOM20

TIM23

PGAM5

FUNDC1 and p-FUNDC1 (p-ser13) were detected by Western blotting.

Results

We cultured primary chondrocytes and established osteoarthritis-like chondrocyte injury model successfully. Compared with the control group

the mRNA and protein levels of Col2α1 were significantly decreased (

0.01)

while the levels of MMP13 were significantly increased (

0.01) in the IL-1β group. The levels of Col2α1 were obviously enhanced (

0.05)

while those of MMP13 were reduced (

0.01) in the IL-1β+FLIPUS group than the IL-1β group. Compared with the control group

the protein expression of LC3B-Ⅱ was remarkably reduced in the IL-1β group (

0.05). The protein levels of LC3B-Ⅱ and PGAM5 were significantly increased (

0.01)

while those of TOM20

TIM23 and p-Ser13 were

notably decreased (

0.05) in the IL-1β+FLIPUS group than the IL-1β group.

Conclusion

FLIPUS may activate the mitophagy in chondrocytes though dephosphorylation of FUNDC1 Ser13

via

up-regulating PGAM5 expression.

关键词

Keywords

references

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⁰

Focused low-intensity pulsed ultrasound promotes chondrocyte mitophagy via up-regulating PGAM5 protein

DOI：10.16016/j.1000-5404.202009202

摘要

Abstract

关键词

Keywords

references