Kinesin family member 3A is involved in inflammatory regulation of asthmatic airway epithelial by binding to β-arrestin in the Wnt/β-catenin pathway

Lili YANG; Yaping WANG; Kunjiao XUE; Lingying RUAN; Zhou FU; Gang GENG

doi:10.16016/j.1000-5404.202009180

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Kinesin family member 3A is involved in inflammatory regulation of asthmatic airway epithelial by binding to β-arrestin in the Wnt/β-catenin pathway

更新时间：2026-01-09

- Kinesin family member 3A is involved in inflammatory regulation of asthmatic airway epithelial by binding to β-arrestin in the Wnt/β-catenin pathway
- Journal of Third Military Medical University Vol. 43, Issue 6, Pages: 496-502(2021)
- 作者机构：
- 作者简介：
- 基金信息：
  
  the General Program of National Natural Science Foundation of China(81670018);the Science and Technology Research Program of Chongqing Municipal Education Commission(KJQN201800407)
- DOI：10.16016/j.1000-5404.202009180
  CLC：
- Received：21 September 2020，
  
  Published：30 March 2021
- 稿件说明：
移动端阅览
Lili YANG, Yaping WANG, Kunjiao XUE, et al. Kinesin family member 3A is involved in inflammatory regulation of asthmatic airway epithelial by binding to β-arrestin in the Wnt/β-catenin pathway[J]. Journal of Third Military Medical University, 2021, 43(6): 496-502.
DOI：

Lili YANG, Yaping WANG, Kunjiao XUE, et al. Kinesin family member 3A is involved in inflammatory regulation of asthmatic airway epithelial by binding to β-arrestin in the Wnt/β-catenin pathway[J]. Journal of Third Military Medical University, 2021, 43(6): 496-502. DOI： 10.16016/j.1000-5404.202009180.

摘要

Abstract

Objective

To investigate the possible mechanism of kinesin family member 3A (KIF3A) taking part in the regulation of asthma airway inflammation

so as to provide a new target and direction for asthma treatment.

Methods

Female C57BL/6 mice were randomly divided into asthma group (

=10) and control group (

=10). Western blotting and immunohistochemistry (IHC) were performed to detect the expression of KIF3A in the 2 groups. After human bronchial epithelial 16HBE cells were intervened using phosphate buffer solution (PBS) and house dust mite (HDM)

Western blotting and RT-PCR were adopted to detect the KIF3A expression. Moreover

the lentiviral vectors of KIF3A overexpression (LV-KIF3A-45894-J2

LV-KIF3A) and knock-down (LV-KIF3A-RNAi-81183-1

RNAi) were constructed and then transfected into 16HBE cells

and the blank lentiviral vectors and those containing negative control sequence served as controls

respectively. The binding of KIF3A to β-arrestin in the overexpressed cells was detected by immunoprecipitation

while the expression level of β-catenin in each group of cells was determined by Western blotting and RT-PCR. The effect of differential expression of KIF3A on the expression of chemokines CCL-17 and CCL-26 was also determined.

Results

In animal models

the expression of KIF3A was significantly lower in the asthma mice than the control mice (

0.05).

In vitro

study indicated that HDM intervention decreased the expression of KIF3A at protein and mRNA levels in 16HBE cells

though there was no statistical difference (

0.05). The RT-PCR and Western blotting results showed that the expression of KIF3A were increased in the LV-KIF3A transfected cells (

0.05) and decreased in the RNAi treated cells (

0.05). Immunoprecipitation confirmed the binding of KIF3A to β-arrestin. No significant difference was fou

nd in the total amount of β-catenin protein between the LV-KIF3A transfected cells and the RNAi treated cells. Compared with the corresponding cells

the mRNA level of β-catenin was significantly lower in the RNAi treated cells (

0.0001)

but no such difference was seen in the LV-KIF3A transfected cells. The RT-PCR results suggested that the levels of chemokines CCL-17 and CCL-26 were increased in the RNAi treated cells (

0.001)

while decreased in the LV-KIF3A transfected cells (

0.05) as compared with their control cells.

Conclusion

KIF3A binds to β-arrestin in the Wnt/β-catenin pathway

and is involved in asthmatic airway epithelial inflammation by regulating the expression of chemokines CCL-17 and CCL-26.

关键词

Keywords

references

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