Long non-coding RNA GASL1 promotes proliferation of vascular smooth muscle cells through Wnt/β-catenin signaling pathway

Yanting LIU; Ran LUO; Zheng SUN; Chunlei TIAN; Xiongwei WANG

doi:10.16016/j.1000-5404.202009065

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Long non-coding RNA GASL1 promotes proliferation of vascular smooth muscle cells through Wnt/β-catenin signaling pathway

更新时间：2026-01-09

- Long non-coding RNA GASL1 promotes proliferation of vascular smooth muscle cells through Wnt/β-catenin signaling pathway
- Journal of Third Military Medical University Vol. 43, Issue 5, Pages: 411-417(2021)
- 作者机构：
- 作者简介：
- 基金信息：
  
  the General Program of National Natural Science Foundation of China(81671155);the General Project of Hubei Provincial Health Commission(WJ2019M063);the Foundation of Medical and Health Research Projects of Yichang City(A18-301-03)
- DOI：10.16016/j.1000-5404.202009065
  CLC：
- Received：10 October 2020，
  
  Published：15 March 2021
- 稿件说明：
移动端阅览
Yanting LIU, Ran LUO, Zheng SUN, et al. Long non-coding RNA GASL1 promotes proliferation of vascular smooth muscle cells through Wnt/β-catenin signaling pathway[J]. Journal of Third Military Medical University, 2021, 43(5): 411-417.
DOI：

Yanting LIU, Ran LUO, Zheng SUN, et al. Long non-coding RNA GASL1 promotes proliferation of vascular smooth muscle cells through Wnt/β-catenin signaling pathway[J]. Journal of Third Military Medical University, 2021, 43(5): 411-417. DOI： 10.16016/j.1000-5404.202009065.

摘要

Abstract

Objective

To investigate whether long non-coding (lnc) RNA GASL1 can promote the proliferation of vascular smooth muscle cells (VSMCs) by activating the Wnt/β-catenin signaling pathway.

Methods

The mRNAs co-expression network related to lncRNA GASL1 was constructed and analyzed with Pathway analysis to predict the signaling pathway of the highest correlation with lncRNA GasL1. After LncRNA GASL1 and lncRNA negative control (lncRNA NC) were transfected into VSMCs respectively

cell counting kit-8 (CCK-8) assay was performed to detect VSMCs viability. Immunofluorescence assay was performed to determine the expression level of proliferation-related protein Bcl-2

and Western blotting to detect the levels of the proteins of Wnt/β-catenin signaling pathway. Finally

Wnt/β-catenin pathway inhibitor ICG001 was added into VSMCs along with lncRNA GASL1 for co-culturing (lncRNA GASL1+ ICG001 group)

and the alterations of VSMCs viability and bcl-2 protein level were detected and compared with those in the lncRNA GASL1+ control group.

Results

There were 374 mRNAs associated with lncRNA GASL1

of which the Wnt/β-catenin pathway had the highest correlation. CCK-8 assay showed that the VSMCs viability was higher in the lncRNA GASL1 group than in the lncRNA NC group (

0.05). The results of immunofluorescence assay and Western blotting revealed that both the Bcl-2 protein level and the levels of Wnt1 and β-catenin were increased in lncRNA GASL1 group (

0.05) when compared to the lncRNA-NC group. After co-culturing with lncRNA GASL1 and ICG001

the VSMCs viability was significantly decreased (

0.05)

and the Bcl-2 protein level was decreased when compared with the the lncRNA GASL1+control group (

0.05).

Conclusion

LncRNA GASL1 promotes the proliferation of VSMCs through the Wnt/β-catenin signaling pathway. Blocking the signaling pathway can reduce the promotive effect of lncRNA GASL1 on VSMCs.

关键词

Keywords

references

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