Non-thermal effects of 0.22 terahertz electromagnetic radiation exposure-induced injury in Neuro-2a cells

Qinlong MA; Chunhai CHEN; Min LIN; Jiawen TAO; Ping DENG; Peng GAO; Yonghui LU; Huifeng PI; Mindi HE; Lei ZHANG; Yanwen ZHANG; Zhengping YU

doi:10.16016/j.1000-5404.202008145

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Non-thermal effects of 0.22 terahertz electromagnetic radiation exposure-induced injury in Neuro-2a cells

Monographic Reports: Biological effects of terahertz radiation | 更新时间：2026-01-09

- Non-thermal effects of 0.22 terahertz electromagnetic radiation exposure-induced injury in Neuro-2a cells
- Journal of Third Military Medical University Vol. 43, Issue 23, Pages: 2267-2273, 2289(2020)
- 作者机构：
  
  400038 重庆，陆军军医大学(第三军医大学)军事预防医学系军队劳动卫生学教研室，电磁辐射医学防护教育部重点实验室
- 作者简介：
- 基金信息：
  
  the National Key R & D Program of China(2017YFC0108601);the General Program of National Natural Science Foundation of China(31870842)
- DOI：10.16016/j.1000-5404.202008145
  CLC： R-334;R146;R730.264
- Received：18 August 2020，
  
  Revised：2020-09-25，
  
  Published：15 December 2020
- 稿件说明：
移动端阅览
Qinlong MA, Chunhai CHEN, Min LIN, et al. Non-thermal effects of 0.22 terahertz electromagnetic radiation exposure-induced injury in Neuro-2a cells[J]. Journal of Third Military Medical University, 2020, 43(23): 2267-2273, 2289.
DOI：

Qinlong MA, Chunhai CHEN, Min LIN, et al. Non-thermal effects of 0.22 terahertz electromagnetic radiation exposure-induced injury in Neuro-2a cells[J]. Journal of Third Military Medical University, 2020, 43(23): 2267-2273, 2289. DOI： 10.16016/j.1000-5404.202008145.

摘要

目的

探讨0.22太赫兹(terahertz，THz)电磁辐射暴露致小鼠神经母细胞瘤细胞株——Neuro-2a细胞损伤的非热效应特点。

方法

以Neuro-2a细胞作为体外实验暴露模型，采用频率为0.22 THz，平均功率密度为0(Sham组)、25(25 mW/cm

辐照组)、50 mW/cm

(50 mW/cm

辐照组)辐

照细胞，辐照时间为5 min，监测辐照引起的细胞培养基温度变化；于暴露后24 h检测细胞增殖(EdU阳性细胞比例和细胞活力)、凋亡(γH2AX、TUNEL阳性细胞比例、

Bax

与

Bcl2

基因表达水平)；于暴露后48 h分析细胞突触生长(突触长度、分支数目及

Tubb3

与

Syp

基因表达水平)的变化情况。

结果

与Sham组比较，两辐照组细胞培养基温度升高在0.1~0.4 ℃；EdU阳性细胞比例、细胞活力、γH2AX和TUNEL阳性细胞比例及

Bax

、

Bcl2

基因表达水平均无明显改变；仅50 mW/cm

辐照组细胞突触长度和分支数目较Sham组明显减少(

0.01)，

Tubb3

和

Syp

基因表达也明显降低(

0.05)。

结论

THz辐射暴露以非热效应为主，可抑制Neuro-2a细胞突触生长，而不影响细胞增殖和凋亡，提示神经细胞突触生长是THz辐射产生神经生物效应的敏感靶标。

Abstract

Objective

To explore the features of non-thermal effects of cell injury induced by terahertz (THz) radiation at a frequency of 0.22 THz

in a mouse neuroblastoma cell line

Neuro-2a.

Methods

The cultured Neuro-2a cells were exposed to 0.22 THz irradiation at average power densities of 0 (sham group)

and 25 and 50 mW/cm

(radiation groups)

for 5 min

respectively. The temperature changes of culture medium were monitored. The cell proliferation and apoptosis and the mRNA expression of

Bax

and

Bcl

2 at 24 h after THz exposure were detected by measuring the ratio of EdU

cells

CCK-8 assay for cell viability

immunofluorescence assay for γH2AX

cells

TUNEL assay

and real-time PCR. The neurite length

number of branch points

and mRNA expression of

Tubb

3 and

Syp

were analyzed in the Neuro-2a cells at 48 h after THz exposure.

Results

Compared with the sham group

the temperature of culture medium in the radiation groups was increased by a range from 0.1 to 0.4 ℃. There were no significant differences in the cell viability

ratios of positive cells for EdU

γH2AX and TUNEL

or the mRNA expression levels of

Bax

and

Bcl

2. The neurite length and number of branch points were obviously reduced (

0.01)

and the mRNA levels of

Tubb

3 and

Syp

were also notably down-regulated (

0.05) in the 50 mW/cm

group.

Conclusion

THz radiation mainly exhibits non-thermal effects

and can inhibit neurite outgrowth but not affect the proliferation and apoptosis in Neuro-2a cells. Our findings suggest that neurite outgrowth is a sensitive target of neurobiological effects after THz radiation exposure.

关键词

Keywords

references

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