Effects of terahertz exposure on skin injury in mouse model

Chunhai CHEN; Qinlong MA; Jiawen TAO; Yonghui LU; Min LIN; Peng GAO; Ping DENG; Mindi HE; Huifeng PI; Lei ZHANG; Yanwen ZHANG; Zhengping YU

doi:10.16016/j.1000-5404.202008138

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Effects of terahertz exposure on skin injury in mouse model

Monographic Reports: Biological effects of terahertz radiation | 更新时间：2026-01-09

- Effects of terahertz exposure on skin injury in mouse model
- Journal of Third Military Medical University Vol. 43, Issue 23, Pages: 2282-2289(2020)
- 作者机构：
  
  400038 重庆，陆军军医大学(第三军医大学)军事预防医学系军队劳动卫生学教研室，电磁辐射医学防护教育部重点实验室
- 作者简介：
- 基金信息：
  
  the National Key R & D Program of China(2017YFC0108601);the General Program of National Natural Science Foundation of China(31870842)
- DOI：10.16016/j.1000-5404.202008138
  CLC： R-334;R146;R758.13
- Received：18 August 2020，
  
  Revised：2020-09-27，
  
  Published：15 December 2020
- 稿件说明：
移动端阅览
Chunhai CHEN, Qinlong MA, Jiawen TAO, et al. Effects of terahertz exposure on skin injury in mouse model[J]. Journal of Third Military Medical University, 2020, 43(23): 2282-2289.
DOI：

Chunhai CHEN, Qinlong MA, Jiawen TAO, et al. Effects of terahertz exposure on skin injury in mouse model[J]. Journal of Third Military Medical University, 2020, 43(23): 2282-2289. DOI： 10.16016/j.1000-5404.202008138.

摘要

目的

探讨太赫兹(terahertz，THz)辐射暴露所致皮肤组织损伤的生物效应特点及其潜在的分子机制。

方法

采用C57BL/6J小鼠为实验动物模型，通过频率为0.22 THz，平均功率密度为0、25、50 mW/cm

的THz辐照动物，辐照时间为5 min，观察辐照引起的暴露部位皮肤升温曲线特点和皮肤组织病理损伤特征，同时通过对辐照部位皮肤组织进行转录组测序及生物信息学分析，揭示损伤的整体效应及潜在分子机制，并进一步通过ELISA验证RNA-seq揭示的相关效应。

结果

25 mW/cm

THz辐照不引起明显的升温效应，但50 mW/cm

THz辐照能够导致辐照动物皮肤平均温度迅速升高超过2 ℃

并且导致辐照部位皮肤组织出现上皮脱落及出血等病理损伤特征。通过对损伤皮肤组织进行转录组测序，共筛选出上调基因49个，下调基因27个。对这些基因进行GO及KEGG分析结果显示，其主要功能为调控机体组织免疫及炎症反应等。ELISA检测结果显示，THz暴露后皮肤炎症因子IL-1β、IL-6及TNF-α表达明显升高(

0.05

0.01)。

结论

THz辐射导致暴露部位皮肤出现明显的热效应损伤及引起炎症反应等损伤效应，并导致一系列基因表达异常。

Abstract

Objective

To explore the characteristics of biological effects of terahertz (THz) exposure on the skin injury

and investigate the underlying mechanisms.

Methods

The hair-removed skin of C57BL/6J mice were exposed to 0.22 THz irradiation

at an average power density of 0

25 and 50 mW/cm

respectively

for 5 min. The temperature curve and pathological changes in the exposed area were observed and analyzed. The gene expression in the skin area was detected using RNA-seq analysis and bioinformatics analysis for global changes of the impairment and underlying molecular mechanisms. Finally

ELISA was used to verify inflammatory responses in the skin revealed by RNA-seq.

Results

Though 25 mW/cm

THz irradiation did not induce obvious temperature change

50 mW/cm

THz irradiation exposure significantly raised the temperature higher than 2 ℃

and caused epithelial detachment and bleeding in the exposed area. RNA-seq analysis found 49 genes up-regulated and 27 genes down-regulated in the injured skin area. The following Gene Ontology (GO) analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis revealed that these genes were majorly involved in immune responses and inflammatory responses. The results of ELISA indicated that the expression levels of IL-1β

IL-6

and TNF-α were increased remarkably after exposure.

Conclusion

THz irradiation exposure causes significant thermal effects and other injuries

including inflammatory responses

and induces abnormal expression of a series of genes in the exposed skin area.

关键词

Keywords

references

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