Association of plasma mtDNA level with cardiopulmonary bypass-related inflammation in ICU patients

Jingxiu FAN; Jia HU; Yingqiang GUO; Yan KANG

doi:10.16016/j.1000-5404.202007081

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Association of plasma mtDNA level with cardiopulmonary bypass-related inflammation in ICU patients

Clinical Medicine | 更新时间：2026-01-09

- Association of plasma mtDNA level with cardiopulmonary bypass-related inflammation in ICU patients
- Journal of Third Military Medical University Vol. 42, Issue 24, Pages: 2403-2407(2020)
- 作者机构：
  
  1.610041 成都，四川大学华西医院心脏大血管外科
  2.610041 成都，四川大学华西医院重症医学科
- 作者简介：
- 基金信息：
  
  the General Program of National Natural Science Foundation of China(81670327);the Key Project of Sichuan Provincial Department of Science and Technology(2020YFS0163)
- DOI：10.16016/j.1000-5404.202007081
  CLC： R364.5;R446.11;R654.1
- Received：08 July 2020，
  
  Revised：2020-08-10，
  
  Published：30 December 2020
- 稿件说明：
移动端阅览
Jingxiu FAN, Jia HU, Yingqiang GUO, et al. Association of plasma mtDNA level with cardiopulmonary bypass-related inflammation in ICU patients[J]. Journal of Third Military Medical University, 2020, 42(24): 2403-2407.
DOI：

Jingxiu FAN, Jia HU, Yingqiang GUO, et al. Association of plasma mtDNA level with cardiopulmonary bypass-related inflammation in ICU patients[J]. Journal of Third Military Medical University, 2020, 42(24): 2403-2407. DOI： 10.16016/j.1000-5404.202007081.

摘要

目的

探究体外循环心脏术后ICU患者血浆mtDNA水平与炎症细胞计数和炎症因子表达之间的潜在联系。

方法

2015年9月1日到2016年12月1日，华西医院心外科行体外循环心脏手术患者，返回ICU半小时内采集血常规、肝功和肾功、NT-proBNP、IL-6、CRP、PCT和mtDNA。规范完成检测和结果判定及录入，SPSS21.0进行统计分析。

结果

① 纳入患者962名，男性459名，女性503名。②血浆mtDNA水平对数值与体外循环心脏术后血小板活化程度对数值之间存在直线相关(

=0.102 7，

=0.002 8)。③血浆mtDNA水平平方根数值与体外循环心脏术后中性粒细胞绝对值平方根数值之间存在直线相关(

=0.106 9，

=0.0040)。④血浆mtDNA水平对数值与体外循环心脏术后IL-6对数值之间存在直线相关(

=0.0798，

=0.045 3)。

结论

血浆mtDNA水平与体外循环心脏术后早期患者的全身炎症反应密切相关。体外循环过程中活化炎症细胞可能是血浆mtDNA的释放来源。

Abstract

Objective

To explore the potential association of plasma mtDNA level with inflammatory cell counts and inflammatory factor levels in ICU patients after cardiac surgery under cardiopulmonary bypass (CPB).

Methods

From September 1

2015 to December 1

2016

all the patients who underwent cardiac surgery under CPB at our department of cardiovascular surgery were recruited in this study. Blood routine

liver and renal functions

and the levels of N-terminal brain natriuretic propeptide (NT-proBNP)

IL-6

C-reactive protein (CRP)

procalcitonin (PCT)

and mtDNA were tested and measured within half an hour after cardiac surgery according to the respective requirements. Statistical software SPSS 21.0 was used for statistical analysis.

Results

① A total of 962 patients were included in this study

including 459 males and 503 females. ② A linear correlation was observed between the logarithm of plasma mtDNA level and the logarithm of platelet activation after cardiac surgery under CPB (

=0.102 7

=0.002 8). ③The square root of the plasma mtDNA level had a linear correlation with the square root of the absolute neutrophil number after cardiac surgery under CPB (

=0.106 9

=0.004 0). ④There was a linear correlation between the logarithm of plasma mtDNA levels and the logarithm of IL-6 after cardiac surgery under CPB (

=0.079 8

=0.045 3).

Conclusion

Plasma mtDNA level is closely associated with systemic inflammatory responses in ICU post-CPB patients at early stage. Activation of inflammatory cells in the CPB process may be the source of plasma mtDNA release

关键词

Keywords

references

LANDIS R C. Redefining the systemic inflammatory response[J].Semin Cardiothorac Vasc Anesth, 2009, 13(2): 87-94. DOI:10.1177/1089253209337743.

HUFFMYER J L, GROVES D S. Pulmonary complications of cardiopulmonary bypass[J]. Best Pract Res Clin Anaesthesiol, 2015, 29(2): 163-175. DOI: 10.1016/j.bpa.2015.04.002.

HERBERT C, PATEL M, NUGENT A, et al . Serum cystatin C as an early marker of neutrophil gelatinase-associatedlipocalin-positive acute kidney injury resulting from cardiopulmonary bypass in infants with congenital heart disease[J ] . Congenit Heart Dis, 2015, 10(4): E180-E188. DOI:10.1111/chd.12253.

GANUSHCHAK Y M, FRANSEN E J, VISSER C, et al . Neurological complications after coronary artery bypass grafting related to the performance of cardiopulmonary bypass[J ] . Chest, 2004, 125(6): 2196-2205. DOI:10.1378/chest.125.6.2196.

BLIKSØEN M, MARIERO L H, OHM I K, et al . Increased circulating mitochondrial DNA after myocardial infarction[J ] .Int J Cardiol, 2012, 158(1): 132-134. DOI:10.1016/j.ijcard.2012.04.047.

WANG L, XIE L, ZHANG Q G, et al . Plasma nuclear and mitochondrial DNA levels in acute myocardial infarction patients[J ] . Coron Artery Dis, 2015, 26(4): 296-300. DOI:10.1097/MCA.0000000000000231.

QIN C Y, GU J, LIU R Q, et al . Release of mitochondrial DNA correlates with peak inflammatory cytokines in patients with acute myocardial infarction[J ] .Anatol J Cardiol, 2017, 17(3): 224-228. DOI:10.14744/AnatolJCardiol.2016.7209.

CAIELLI S, ATHALE S, DOMIC B, et al . Oxidized mitochondrial nucleoids released by neutrophils drive type I interferon production in human lupus[J ] . J Exp Med, 2016, 213(5): 697-713. DOI:10.1084/jem.20151876.

秦超毅, 古君, 钱宏, 等.体外循环围手术期血液中线粒体DNA浓度变化[J].中国胸心血管外科临床杂志, 2015, 22(3): 241-244.

QIN C Y, GU J, QIAN H, et al . Variation of perioperative mitochondrial DNA concentration after cardiopulmonary bypass[J ] . Chin J Clin Thorac Cardiovasc Surg, 2015, 22(3): 241-244.

NISSANKA N, MORAES C T. Mitochondrial DNAheteroplasmy in disease and targeted nuclease-based therapeutic approaches[J]. EMBO Rep, 2020, 21(3): e49612. DOI: 10.15252/embr.201949612.

范景秀, 朱慧莉.线粒体DNA致病作用的研究进展[J].中国胸心血管外科临床杂志, 2020, 27(10): 1242-1246.

FAN J X, ZHU H L. Research progress of mitochondrial DNA as a pathogenic factor in diseases[J]. Chin J Clin Thorac Cardiov Surg, 2020, 27(10): 1242-1246.

BAYSA A, FEDOROV A, KONDRATOV K, et al . Release of mitochondrial and nuclear DNA during on-pump heart surgery: Kinetics and relation to extracellular vesicles[J ] . J Cardiovasc Transl Res, 2019, 12(3): 184-192.

CHO S Y, JEON Y L, KIM W, et al . Mean platelet volume and mean platelet volume/platelet count ratio in infective endocarditis[J ] . Platelets, 2014, 25(8): 559-561. DOI: 10.3109/09537104.2013.857394.

SNIECINSKI R M, CHANDLER W L. Activation of the hemostatic system during cardiopulmonary bypass[J]. Anesth Analg, 2011, 113(6): 1319-1333. DOI: 10.1213/ANE.0b013e3182354b7e.

QIN C Y, GU J, HU J, et al . Platelets activation is associated with elevated plasma mitochondrial DNA during cardiopulmonary bypass[J ] . J Cardiothorac Surg, 2016, 11(1): 1-9. DOI: 10.1186/s13019-016-0481-4.

ZARBOCK A, POLANOWSKA-GRABOWSKA R K, LEY K. Platelet-neutrophil-interactions: linking hemostasis and inflammation[J]. Blood Rev, 2007, 21(2): 99-111. DOI: 10.1016/j.blre.2006.06.001.

VARON D, SHAI E. Platelets and their microparticles as key players in pathophysiological responses[J]. J Thromb Haemost, 2015, 13: S40-S46. DOI: 10.1111/jth.12976.

CANGEMI R, CASCIARO M, ROSSI E, et al . Platelet activation is associated with myocardial infarction in patients with pneumonia[J ] . J Am Coll Cardiol, 2014, 64(18): 1917-1925. DOI: 10.1016/j.jacc.2014.07.985.

BOUDREAU L H, DUCHEZ A C, CLOUTIER N, et al . Platelets release mitochondria serving as substrate for bactericidal group ⅡA-secreted phospholipase A2 to promote inflammation[J ] . Blood, 2014, 124(14): 2173-2183. DOI: 10.1182/blood-2014-05-573543.

INOUE N, OKA N, KITAMURA T, et al . Neutrophil elastase inhibitor sivelestat attenuates perioperative inflammatory response in pediatric heart surgery with cardiopulmonary bypass[J ] . Int Heart J, 2013, 54(3): 149-153. DOI: 10.1536/ihj.54.149.

RINDER C S, SMITH M J, RINDER H M, et al . Leukocyte effects of C5a-receptor blockade during simulated extracorporeal circulation[J ] . Ann Thorac Surg, 2007, 83(1): 146-152. DOI: 10.1016/j.athoracsur.2006.08.019.

YOUSEFI S, MIHALACHE C, KOZLOWSKI E, et al . Viable neutrophils release mitochondrial DNA to form neutrophil extracellular traps[J ] . Cell Death Differ, 2009, 16(11): 1438-1444. DOI: 10.1038/cdd.2009.96.

BRINKMANN V, ZYCHLINSKY A. Neutrophil extracellular traps: is immunity the second function of chromatin?[J]. J Cell Biol, 2012, 198(5): 773-783. DOI: 10.1083/jcb.201203170.

KRYCHTIUK K A, RUHITTEL S, HOHENSINNER P J, et al . Mitochondrial DNA and toll-like receptor-9 are associated with mortality in critically ill patients[J ] . Crit Care Med, 2015, 43(12): 2633-2641. DOI: 10.1097/CCM.0000000000001311.

TAMURA Y, NOMURA A, YOSHIDA S, et al . Quick Sepsis-related organ failure assessment score as a possible predictor for in-hospital adverse events in infective endocarditis[J ] . Acute Med Surg, 2019, 6(2): 138-144. DOI: 10.1002/ams2.393.

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