Application of folate receptor-positive circulating tumor cells in auxiliary diagnosis of malignant pulmonary nodules

Bo LIU; Lu HUANG; Ya CAO; Qiulin TAN; Yali WANG; Qiaonan GUO

doi:10.16016/j.1000-5404.202007074

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Application of folate receptor-positive circulating tumor cells in auxiliary diagnosis of malignant pulmonary nodules

Clinical Medicine | 更新时间：2026-01-09

- Application of folate receptor-positive circulating tumor cells in auxiliary diagnosis of malignant pulmonary nodules
- Journal of Third Military Medical University Vol. 43, Issue 23, Pages: 2330-2335(2020)
- 作者机构：
  
  400037 重庆，陆军军医大学(第三军医大学)第二附属医院病理科
- 作者简介：
- 基金信息：
  
  the National Natural Science Foundation for Young Scholars of China(31801664)
- DOI：10.16016/j.1000-5404.202007074
  CLC： R446.119;R730.43;R734.2
- Received：09 July 2020，
  
  Revised：2020-09-24，
  
  Published：15 December 2020
- 稿件说明：
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Bo LIU, Lu HUANG, Ya CAO, et al. Application of folate receptor-positive circulating tumor cells in auxiliary diagnosis of malignant pulmonary nodules[J]. Journal of Third Military Medical University, 2020, 43(23): 2330-2335.
DOI：

Bo LIU, Lu HUANG, Ya CAO, et al. Application of folate receptor-positive circulating tumor cells in auxiliary diagnosis of malignant pulmonary nodules[J]. Journal of Third Military Medical University, 2020, 43(23): 2330-2335. DOI： 10.16016/j.1000-5404.202007074.

摘要

目的

评价靶向叶酸受体(folate receptor

FR)阳性循环肿瘤细胞(circulating tumor cell

CTC)检测方法在临床肺部结节良恶性诊断中的实用价值。

方法

选取2017年5月至2018年4月本院收治的肺部结节筛查患者288例，采用靶向FR CTC技术检测外周血中CTC含量；结合患者术后的病理检查结果，分析靶向FR CTC检测在肺部恶性肿瘤辅助诊断中的准确性、可行性。

结果

以8.7 Folate Units/3 mL为cut-off值，136例肺部恶性肿瘤的CTC水平显著高于152例肺部良性疾病患者(

0.05)。136例肺部恶性肿瘤FR阳性CTC 107例，152例良性病变结节FR假阳性CTC 44例，总体上靶向FR CTC检测技术对肺部恶性肿瘤的检测灵敏度为78.7%，特异性为71.1%。特别是在Ⅰ期肺癌灵敏度达到76.9%，9例原位肺癌CTC检测均呈阳性，与肿瘤标志物、CT检查等比较，CTC检测对早期肺癌具有较高的辅助诊断价值。

结论

靶向FR CTC检测可以应用于肺部结节良恶性的筛查，对肺部恶性肿瘤的早发现有较好的辅助诊断价值。

Abstract

Objective

To evaluate the practical value of detecting folate receptor (FR)-positive circulating tumor cells (CTC) in clinical diagnosis of benign and malignant lung nodules.

Methods

A total of 288 patients with pulmonary nodules admitted in our hospital from May 2017 to April 2018 were enrolled in this study. FR-targeted CTC technology was used to quantitatively detect CTC content in peripheral blood samples. The results were analyzed with the postoperative pathological results to evaluate the accuracy and feasibility of the detection in the auxiliary diagnosis of lung malignant tumors.

Results

With a cut-off value of 8.7 Folate Units/3 mL as the threshold

the CTC content was significantly higher in the 136 patients with pulmonary malignant tumor than the 152 patients with pulmonary benign disease (

0.05). Among the 136 patients with pathologically diagnosed pulmonary malignant tumors

FR-positive CTC were observed in 106 patients. In the 152 patients with benign nodules

44 cases showed false positive CTC results. In general

the sensitivity and specificity of FR-positive CTC detection was 78.7% and 71.1%

respectively in the detection of pulmonary malignant tumors. Furthermore

the sensitivity reached 76.9% in stage I lung cancer (70/91)

and all 9 cases of lung cancer

in situ

were positive in the detection. Compared with tumor markers and CT examinations

FR-positive CTC detection was of higher auxiliary diagnostic value for early lung cancer.

Conclusion

FR-positive CTC detection can be used to screen benign and malignant lung nodules

and has a good value in the diagnosis of early-stage pulmonary malignant tumors.

关键词

Keywords

references

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