Effects of caffeine consumption on cutaneous wound healing and scar formation in mice

Xing CHEN; Fen LUO; Zhizhong HUANG; Yan PENG; Renping XIONG; Ping LI

doi:10.16016/j.1000-5404.202006248

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Effects of caffeine consumption on cutaneous wound healing and scar formation in mice

更新时间：2026-01-09

- Effects of caffeine consumption on cutaneous wound healing and scar formation in mice
- Journal of Third Military Medical University Vol. 43, Issue 1, Pages: 75-82(2021)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.16016/j.1000-5404.202006248
  CLC：
- Received：29 June 2020，
  
  Published：15 January 2021
- 稿件说明：
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Xing CHEN, Fen LUO, Zhizhong HUANG, et al. Effects of caffeine consumption on cutaneous wound healing and scar formation in mice[J]. Journal of Third Military Medical University, 2021, 43(1): 75-82.
DOI：

Xing CHEN, Fen LUO, Zhizhong HUANG, et al. Effects of caffeine consumption on cutaneous wound healing and scar formation in mice[J]. Journal of Third Military Medical University, 2021, 43(1): 75-82. DOI： 10.16016/j.1000-5404.202006248.

摘要

Abstract

Objective

To investigate the effect of caffeine consumption on skin wound healing and scar formation in mice.

Methods

The mouse model was established according to our previous method of chronic intervention with caffeine combined with the model of full-thickness skin wound on the back. Wound closure was traced every 2 days in each group using transparent film until the wound closed

and the scar areas were calculated at 30 d after healing. The sections were stained with hematoxylin-eosin (HE) to observe the changes in epithelial cells crawling and thickness of granulation tissue at 3

6 and 9 d after wounding

and scar width and the thinness-to-thickness ratio of the epithelium in the scar tissue at 30 d after healing with the aid of Labwork 4.0 software. Masson staining was used to analyze the collagen changes of the wound at 6 d after wounding and of the scar tissue at 30 d after healing. Immunohistochemical assay was used to observe the changes of CD31 and CD11b protein levels in wound tissue at 6 d after wounding.

Results

Results The healing time of caffeine group was delayed by 28.3%

and the scar area was reduced by 56.8% when compared with that of the control group. In the process of wound healing

the crawling length of epithelium and the thickness of granulation tissue were significantly decreased in the caffeine group than the control group (

0.01)

the collagen content and the expression level of CD31 in the wound were also decreased (

0.01)

and the expression level of CD11b was obviously enhanced (

0.01). Meanwhile

compared with the control group

the caffeine group had significantly decreased scar width (

0.01)

higher thickness of scar tissue epithelium (

0.01)

but similar collagen content in the scar and the surrounding undamaged subcutaneous collagen.

Conclusion

Caffeine consumption can delay the wound healing in mice

but attenuate scar formation.

关键词

Keywords

references

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