LINC00525 targeting miR-338-3p promotes apoptosis and reduces paclitaxel resistance in gastric cancer cells

Yucheng SUN; Xiaowei LIU; Honglei YU

doi:10.16016/j.1000-5404.202006247

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LINC00525 targeting miR-338-3p promotes apoptosis and reduces paclitaxel resistance in gastric cancer cells

Basic Medicine | 更新时间：2026-01-09

- LINC00525 targeting miR-338-3p promotes apoptosis and reduces paclitaxel resistance in gastric cancer cells
- Journal of Third Military Medical University Vol. 42, Issue 22, Pages: 2202-2209(2020)
- 作者机构：
  
  133000 吉林延吉, 延边大学附属医院普外一科
- 作者简介：
- 基金信息：
- DOI：10.16016/j.1000-5404.202006247
  CLC： R73-362;R735.2;R915
- Received：29 June 2020，
  
  Revised：2020-08-13，
  
  Published：30 November 2020
- 稿件说明：
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Yucheng SUN, Xiaowei LIU, Honglei YU. LINC00525 targeting miR-338-3p promotes apoptosis and reduces paclitaxel resistance in gastric cancer cells[J]. Journal of Third Military Medical University, 2020, 42(22): 2202-2209.
DOI：

Yucheng SUN, Xiaowei LIU, Honglei YU. LINC00525 targeting miR-338-3p promotes apoptosis and reduces paclitaxel resistance in gastric cancer cells[J]. Journal of Third Military Medical University, 2020, 42(22): 2202-2209. DOI： 10.16016/j.1000-5404.202006247.

摘要

目的

研究LINC00525靶向miR-338-3p对胃癌细胞凋亡及耐药性的影响。

方法

将紫杉醇耐药胃癌细胞(HGC-27/PTX)分为si-NC组、si-LINC00525组、miR-NC组、miR-338-3p组、si-LINC00525+anti-miR-NC组、si-LINC00525+anti-miR-338-3p组。细胞计数试剂盒8(cell counting kit 8，CCK-8)检测细胞存活率；实时荧光定量PCR(RT-qPCR)检测LINC00525和miR-338-3p表达水平；蛋白质印迹(Western blot)法检测蛋白表达；Annexin V-FITC/PI双染法检测细胞凋亡；Transwell检测细胞迁移和侵袭；双荧光素酶报告实验验证LINC00525和miR-338-3p的靶向关系。

结果

HGC-27和HGC-27/PTX细胞中LINC00525表达水平升高，miR-338-3p表达水平降低。敲除LINC00525和过表达miR-338-3p，caspase-3、P21、E-cadherin表达水平升高，MMP-2表达水平降低，细胞存活率降低，细胞凋亡率升高，细胞迁移、侵袭数降低。LINC00525靶向调控miR-338-3p，抑制miR-338-3p能减弱敲除LINC00525对细胞HGC-27/PTX的存活率、迁移、侵袭及凋亡的影响。

结论

敲除LINC00525通过上调miR-338-3p表达抑制HGC-27/PTX细胞增殖、迁移和侵袭，促进胃癌细胞凋亡，降低胃癌细胞对紫杉醇的耐药性。

Abstract

Objective

To study the effect of modulating the expression of LINC00525 targeting miR-338-3p on apoptosis and drug resistance of gastric cancer cells.

Methods

Paclitaxel-resistant gastric cancer cells (HGC-27/PTX) were transfected with si-NC

si-LINC00525

miR-NC

miR-338-3p

si-LINC00525+anti-miR-NC

or si-LINC00525+anti-miR-338-3p. Cell counting kit 8 (CCK-8) was used to determine the cell survival rate after the treatments. Real-time fluorescence quantitative PCR (RT-qPCR) was performed to detect LINC00525 and miR-338-3p expression levels

and Western blotting was used to detect the protein expression in the cells. The changes in cell apoptosis and migration and invasion abilities were analyzed using annexin V-FITC/PI double staining and Transwell assays. Dual luciferase reporter assay was performed to verify the targeting relationship between LINC00525 and miR-338-3p.

Results

The expression of LINC00525 was increased and that of miR-338-3p was decreased in both in HGC-27 and HGC-27/PTX cells. Knocking down LINC00525 and overexpression of miR-338-3p significantly increased the expression of caspase-3

P21

and E-cadherin

lowered the expression of MMP-2

decreased the cell survival rate

increased the cell apoptotic rate

and suppressed cell migration and invasion. Dual luciferase reporter assay showed that LINC00525 was capable of regulating the targeted miR-338-3p

and inhibition of miR-338-3p obviously attenuated the effects of LINC00525 knockdown on the survival

migration

invasion

and apoptosis of HGC-27/PTX cells.

Conclusion

LINC00525 knockdown can inhibit the proliferation

migration and invasion

promote apoptosis and reduce paclitaxel resistance of HGC-27/PTX cells by up-regulating miR-338-3.

关键词

Keywords

references

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