Transcription profile and heterologous expression of <italic style="font-style: italic">Echinococcus granulosus</italic> aquaporins genes in the encysted protoscolex

Qingqing TAN; Hongye WU; Jian GAO; Kai LI; Junjie FAN; Xiang LI; Peng LIAO; Wenqiao CAO; Bin YE

doi:10.16016/j.1000-5404.202006204

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Transcription profile and heterologous expression of Echinococcus granulosus aquaporins genes in the encysted protoscolex

Basic Medicine | 更新时间：2026-01-09

- Transcription profile and heterologous expression of Echinococcus granulosus aquaporins genes in the encysted protoscolex
- Journal of Third Military Medical University Vol. 42, Issue 21, Pages: 2081-2092(2020)
- 作者机构：
  
  1.400016 重庆，重庆医科大学基础医学院：病原生物学教研室
  3.400016 重庆，重庆医科大学基础医学院：分子医学与肿瘤研究中心
  2.830011 乌鲁木齐，新疆医科大学寄生虫学教研室
- 作者简介：
- 基金信息：
  
  the General Program of National Natural Science Foundation of China(81672045)
- DOI：10.16016/j.1000-5404.202006204
  CLC： R341;R383.33;R394.3
- Received：22 June 2020，
  
  Revised：2020-08-11，
  
  Published：15 November 2020
- 稿件说明：
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Qingqing TAN, Hongye WU, Jian GAO, et al. Transcription profile and heterologous expression of Echinococcus granulosus aquaporins genes in the encysted protoscolex[J]. Journal of Third Military Medical University, 2020, 42(21): 2081-2092.
DOI：

Qingqing TAN, Hongye WU, Jian GAO, et al. Transcription profile and heterologous expression of Echinococcus granulosus aquaporins genes in the encysted protoscolex[J]. Journal of Third Military Medical University, 2020, 42(21): 2081-2092. DOI： 10.16016/j.1000-5404.202006204.

摘要

目的

探索细粒棘球蚴水通道蛋白(

Echinococcus granulosus

aquaporins

AQPs)在棘球蚴液形成过程中的作用。

方法

收集体外培养不同时期的棘球蚴标本和BALB/c小鼠体内接种的继发棘球蚴标本，RT-qPCR方法检测12个

AQPs基因在体内、体外培养不同发育时期的转录表达。以细粒棘球蚴cDNA为模板，扩增

AQP、

AQP1、

AQP3、

AQP4-1、

AQP4-4和

AQP9-1 6个基因编码区片段，同时，通过化学合成方法扩增另外6个

AQPs(

AQPAn.G、

AQPFA-CHIP、

AQP4-2、

AQP4-3、

AQP4-5、

AQP9-2)基因编码区片段，并在非洲爪蟾卵母细胞中检测这12个

AQPs基因的透水功能，Western blot检测

AQPs在卵母细胞膜上的表达。

结果

体外培养的原头蚴及感染小鼠的继发棘球蚴体积均随培养时间的延长而逐渐增大，棘球蚴囊内透明液体增多。RT-qPCR结果显示12个

AQPs基因在体内、体外培养不同发育时间的转录表达水平均较低。注射

AQPs基因的非洲爪蟾卵母细胞的体积变化率和透水系数与阴性对照组差异

无统计学意义，这12个

AQPs基因在卵母细胞中未表现出水通道功能。Western blot检测12个

AQPs基因在卵母细胞总蛋白、胞质蛋白与胞膜蛋白上的表达，结果均未观察到蛋白在预期的水通道蛋白理论分子量处表达。

结论

12个

AQPs基因在原头蚴囊化形成棘球蚴过程中转录表达水平均较低，这些基因在爪蟾卵母细胞的异源表达均未表现出通透水的功能。

Abstract

Objective

To explore the role of

Echinococcus granulosus

aquaporins (

AQPs) in the accumulation of hydatid fluid.

Methods

The secondary hydatids cultured

in vitro

and inoculated in BALB/c mice were collected respectively

and the relative transcription expression levels of 12

AQPs genes from the hydatid cysts cultured

in vitro

and in mice were detected by RT-qPCR. Six gene coding region fragments of

AQP

AQP1

AQP3

AQP4-1

AQP4-4 and

AQP9-1 were amplified using cDNA templates. The other 6 genes coding region fragments of

AQPs (

AQPAn.G

AQPFA-CHIP

AQP4-2

AQP4-3

AQP4-5 and

AQP9-2) were amplified with chemical synthesis. The water permeability of these 12

AQPs genes were detected in

Xenopus laevis

(

X. laevis

)

and the expression of

AQPs on the oocytes membranes was detected by Western blotting.

Results

The volume of hydatid cultured

in vitro

and

in vivo

was increased gradually with the extension of culture time

and there was more and more transparent hydatid fluid in hydatid cyst. The results of RT-qPCR showed that the relative transcription levels of

AQPs genes in the hydatid cysts were very low or even undetectable except for

AQP3 and

AQP9-1 at the beginning of culture

in vitro

. There were no significant differences in relative volume change rates and water permeability coefficients of

X. laevis

oocytes injected with

AQPs genes when compared with the negative control

and these

AQPs genes did not show water channel functions in the oocytes. Western blot assay displayed that there was no expression of

AQPs genes in total

cytoplasmic and membrane protein on the oocytes.

Conclusion

The transcription levels of

AQPs genes are quite low in the encystation process of protoscolex

and their heterologous expression do not show water channel functions in

X. laevis

oocytes.

关键词

Keywords

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Transcription profile and heterologous expression of Echinococcus granulosus aquaporins genes in the encysted protoscolex

DOI：10.16016/j.1000-5404.202006204

摘要

Abstract

关键词

Keywords

references