Notch pathway participates in regulation of c-kit<sup>+</sup> retinal precursor cell division and differentiation in adult mice for repair of NMDA-induced retinal injury

Xi CHEN; Shanshan LI; Lu ZHAO; Ran YOU; Yanling WANG

doi:10.16016/j.1000-5404.202005147

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Notch pathway participates in regulation of c-kit⁺ retinal precursor cell division and differentiation in adult mice for repair of NMDA-induced retinal injury

更新时间：2026-01-09

- Notch pathway participates in regulation of c-kit⁺ retinal precursor cell division and differentiation in adult mice for repair of NMDA-induced retinal injury
- Journal of Third Military Medical University Vol. 43, Issue 1, Pages: 59-67(2021)
- 作者机构：
- 作者简介：
- 基金信息：
  
  the General Program of National Natural Science Foundation of China(81870686);the Beijing Municipal Natural Science Foundation for Youth Scholars(7184201);the Special Funds for Capital Health Improvement and Research(首发2018-1-2021)
- DOI：10.16016/j.1000-5404.202005147
  CLC：
- Received：18 May 2020，
  
  Published：15 January 2021
- 稿件说明：
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Xi CHEN, Shanshan LI, Lu ZHAO, et al. Notch pathway participates in regulation of c-kit⁺ retinal precursor cell division and differentiation in adult mice for repair of NMDA-induced retinal injury[J]. Journal of Third Military Medical University, 2021, 43(1): 59-67.
DOI：

Xi CHEN, Shanshan LI, Lu ZHAO, et al. Notch pathway participates in regulation of c-kit⁺ retinal precursor cell division and differentiation in adult mice for repair of NMDA-induced retinal injury[J]. Journal of Third Military Medical University, 2021, 43(1): 59-67. DOI： 10.16016/j.1000-5404.202005147.

摘要

Abstract

Objective

To analyze the characteristics of c-kit

endogenous retinal precursor cells (RPCs) in the retina of adult mice and their roles in the repair of N-methyl-D-aspartic acid (NMDA)-induced retinal damage.

Methods

Mouse models of NMDA-induced retinal injury were established in 4-week-old adult C57BL/6J mice. Immunofluorescence assay was used to analyze the relationship between c-kit

RPCs and c-kit stem cell factor (SCF)-positive cells and determine the number of c-kit

RPCs and their division activity at 1

2 and 4 weeks after NMDA injury. The mRNA expression levels of Hes1

Hes5

S100b and Pou4f1 in the injured retinal tissues were analyzed using real-time PCR.

Results

Müller cells surrounding the c-kit

RPCs were observed in the inner nuclear layer (INL) of the retina of adult mice. C-kit

RPCs still maintained a low level of cell division activity. Two weeks after NMDA-induced retinal injury

the number of c-kit

RPCs significantly increased in the retina (

0.001) with markedly increased proportions of dividing cells (

=0.016) and c-kit

GAD65/67

cells/c-kit

cells (

0.001). Two weeks after retinal injury

the Notch pathway components Hes1 and Hes5 were up-regulated (

0.001) and the expression levels of S100b and Pou4f1 were augmented significantly in the retina (

0.001).

Conclusion

Two weeks after NMDA-induced retinal injury

c-kit

RPCs in the retina undergo both symmetrical and asymmetrical cell division and then differentiate into GAD65/67

neurons possibly under regulation by the Notch pathway.

关键词

Keywords

references

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⁰

Notch pathway participates in regulation of c-kit+ retinal precursor cell division and differentiation in adult mice for repair of NMDA-induced retinal injury

DOI：10.16016/j.1000-5404.202005147

摘要

Abstract

关键词

Keywords

references

Notch pathway participates in regulation of c-kit⁺ retinal precursor cell division and differentiation in adult mice for repair of NMDA-induced retinal injury